Biomaterials for liver tissue engineering

Hepatology International

Volumn 8, Issue 2, 2014, Pages 185-197

  Jain, Era ^a,b   Damania, Apeksha ^a   Kumar, Ashok ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY KANPUR   (India)

^b Saint Louis University   (United States)

Author keywords

Biomaterials; Extra cellular matrix; Hepatocytes; Liver tissue engineering; Liver based scaffolds

Indexed keywords

BIOMATERIAL; HYALURONIC ACID; HYBRID SCAFFOLD; LIGAND; POLYCAPROLACTONE; POLYMER; TISSUE SCAFFOLD; UNCLASSIFIED DRUG;

BIOARTIFICIAL LIVER; CELL COMPOSITION; CELL INTERACTION; CELL PROLIFERATION; COCULTURE; EXTRACELLULAR MATRIX; FLUID FLOW; HUMAN; LIVER; LIVER CELL; LIVER STRUCTURE; NONHUMAN; OXYGENATION; PHYSICAL CHEMISTRY; POROSITY; PRIORITY JOURNAL; REVIEW; SHEAR STRESS; SYNTHETIC BIOLOGY; TISSUE ENGINEERING;

EID: 84899951852     PISSN: 19360533     EISSN: 19360541     Source Type: Journal    
DOI: 10.1007/s12072-013-9503-7     Document Type: Review

References (117)

1. Owen SC, Shoichet MS. Design of three-dimensional biomimetic scaffolds. J Biomed Mater Res A 2010;94(4):1321-1331
2. Martinez-Hernandez A, Amenta PS. The extracellular matrix in hepatic regeneration. FASEB J 1995;9(14):1401-1410
3. Griffith LG, Swartz MA. Capturing complex 3D tissue physiology in vitro. Nat Rev Mol Cell Biol 2006;7(3):211-224
4. Griffith LG. Emerging design principles in biomaterials and scaffolds for tissue engineering. Ann N Y Acad Sci 2002;961:83-95 (Pubitemid 34721746)
5. Berthiaume F, Moghe PV, Toner M, Yarmush ML. Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: hepatocytes cultured in a sandwich configuration. FASEB J 1996;10(13):1471-1484 (Pubitemid 26384444)
6. Reid LM, Fiorino AS, Sigal SH, Brill S, Holst PA. Extracellular matrix gradients in the space of Disse: relevance to liver biology. Hepatology 1992;15(6):1198-1203
7. Dunn JC, Yarmush ML, Koebe HG, Tompkins RG. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J 1989;3(2):174-177
8. Nahmias Y, Berthiaume F, Yarmush ML. Integration of technologies for hepatic tissue engineering. Adv Biochem Eng Biotechnol 2007;103:309-329
9. Strain AJ, Neuberger JM. A bioartificial liver - state of the art. Science 2002;295(5557):1005-1009
10. Soldatow VY, Lecluyse EL, Griffith LG, Rusyn I. Models for liver toxicity testing. Toxicol Res (Camb) 2013;2(1):23-39
11. LeCluyse EL, Witek RP, Andersen ME, Powers MJ. Organotypic liver culture models: meeting current challenges in toxicity testing. Crit Rev Toxicol 2012;42(6):501-548
12. Sudo R, Chung S, Zervantonakis IK, Vickerman V, Toshimitsu Y, Griffith LG, et al. Transport-mediated angiogenesis in 3D epithelial coculture. FASEB J 2009;23(7):2155-2164
13. Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes. Hepatology 2006;43(2):257-265
14. Grisham JW. In Arias I, Wolkoff AW, Boyer J, Shafritz DA, Fausto N, Alter H, et al., editors. Organizational Principles of The Liver. 5th ed. Singapore: Wiley; 2009. 3-10
15. Ishibashi H, Nakamura M, Komori A, Migita K, Shimoda S. Liver architecture, cell function, and disease. Semin Immunopathol 2009;31(3):399-409
16. Nahmias Y. Oxygen-mediated enhancement of primary hepatocyte metabolism, functional polarization, and gene expression: achieving in vivo levels of drug clearance and HCV infection in vitro. J Hepatol 2010;52:S374
17. Braet F, Wisse E. Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review. Comp Hepatol 2002;1(1):1
18. Smedsrod B, De Bleser PJ, Braet F, Lovisetti P, Vanderkerken K, Wisse E, et al. Cell biology of liver endothelial and Kupffer cells. Gut 1994;35(11):1509-1516 (Pubitemid 24339243)
19. Huebert RC, Shah VH. Hepatic endothelial cells. In Dufour J-F, C P-A, editors. Signaling Pathways in Liver Diseases. Berlin: Springer-Verlag; 2010. 79-91
20. Martinez-Hernandez A, Delgado FM, Amenta PS. The extracellular matrix in hepatic regeneration. Localization of collagen types I, III, IV, laminin, and fibronectin. Lab Invest 1991;64(2):157-166
21. Michalopoulos GK, Bowen WC, Zajac VF, Beer-Stolz D, Watkins S, Kostrubsky V, et al. Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor. Hepatology 1999;29(1):90-100 (Pubitemid 29023991)
22. Stevens KM. Oxygen requirements for liver cells in vitro. Nature 1965;206(980):199
23. Ananthanarayanan A, Narmada BC, Mo X, McMillian M, Yu H. Purpose-driven biomaterials research in liver-tissue engineering. Trends Biotechnol 2011;29(3):110-118
24. Bhatia SN, Balis UJ, Yarmush ML, Toner M. Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells. FASEB J 1999;13(14):1883-1900 (Pubitemid 29517619)
25. Powers MJ, Domansky K, Kaazempur-Mofrad MR, Kalezi A, Capitano A, Upadhyaya A, et al. A microfabricated array bioreactor for perfused 3D liver culture. Biotechnol Bioeng 2002;78(3):257-269 (Pubitemid 34712370)
26. Allen JW, Khetani SR, Bhatia SN. In vitro zonation and toxicity in a hepatocyte bioreactor. Toxicol Sci 2005;84(1):110-119 (Pubitemid 40340278)
27. Moghe PV, Coger RN, Toner M, Yarmush ML. Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel. Biotechnol Bioeng 1997;56(6):706-711 (Pubitemid 27505983)
28. Chen AA, Thomas DK, Ong LL, Schwartz RE, Golub TR, Bhatia SN. Humanized mice with ectopic artificial liver tissues. Proc Natl Acad Sci USA 2011;108(29):11842-11847
29. Du Y, Chia SM, Han R, Chang S, Tang H, Yu H. 3D hepatocyte monolayer on hybrid RGD/galactose substratum. Biomaterials 2006;27(33):5669-5680 (Pubitemid 44294821)
30. Chen AA, Khetani SR, Lee S, Bhatia SN, Van Vliet KJ. Modulation of hepatocyte phenotype in vitro via chemomechanical tuning of polyelectrolyte multilayers. Biomaterials 2009;30(6):1113-1120
31. Mooney D, Hansen L, Vacanti J, Langer R, Farmer S, Ingber D. Switching from differentiation to growth in hepatocytes: control by extracellular matrix. J Cell Physiol 1992;151(3):497-505
32. Michalopoulos G, Cianciulli HD, Novotny AR, Kligerman AD, Strom SC, Jirtle RL. Liver regeneration studies with rat hepatocytes in primary culture. Cancer Res 1982;42(11):4673-4682 (Pubitemid 13205972)
33. Zhao Y, Xu Y, Zhang B, Wu X, Xu F, Liang W, et al. In vivo generation of thick, vascularized hepatic tissue from collagen hydrogel-based hepatic units. Tissue Eng C 2010;16(4):653-659
34. Li JL, Pan JL, Zhang LG, Guo XJ, Yu YT. Culture of primary rat hepatocytes within porous chitosan scaffolds. J Biomed Mater Res A 2003;67A(3):938-943
35. Li KG, Wang Y, Miao ZC, Xu DY, Tang YF, Feng MF. Chitosan/gelatin composite microcarrier for hepatocyte culture. Biotechnol Lett 2004;26(11):879-883 (Pubitemid 38982840)
36. Elcin YM, Dixit V, Gitnick G. Hepatocyte attachment on biodegradable modified chitosan membranes: in vitro evaluation for the development of liver organoids. Artif Organs 1998;22(10):837-846 (Pubitemid 28471274)
37. Lee KH, Shin SJ, Kim CB, Kim JK, Cho YW, Chung BG, et al. Microfluidic synthesis of pure chitosan microfibers for bioartificial liver chip. Lab Chip 2010;10(10):1328-1334
38. Feng ZQ, Chu X, Huang NP, Wang T, Wang Y, Shi X, et al. The effect of nanofibrous galactosylated chitosan scaffolds on the formation of rat primary hepatocyte aggregates and the maintenance of liver function. Biomaterials 2009;30(14):2753-2763
39. Dvir-Ginzberg M, Gamlieli-Bonshtein I, Agbaria R, Cohen S. Liver tissue engineering within alginate scaffolds: effects of cell-seeding density on hepatocyte viability, morphology, and function. Tissue Eng 2003;9(4):757-766 (Pubitemid 37048116)
40. Glicklis R, Shapiro L, Agbaria R, Merchuk JC, Cohen S. Hepatocyte behavior within three-dimensional porous alginate scaffolds. Biotechnol Bioeng 2000;67(3):344-353 (Pubitemid 30051332)
41. Miranda JP, Rodrigues A, Tostoes RM, Leite S, Zimmerman H, Carrondo MJ, et al. Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors. Tissue Eng C 2010;16(6):1223-1232
42. Seo SJ, Kim IY, Choi YJ, Akaike T, Cho CS. Enhanced liver functions of hepatocytes cocultured with NIH 3T3 in the alginate/galactosylated chitosan scaffold. Biomaterials 2006;27(8):1487-1495 (Pubitemid 41739575)
43. Wang X, Yan Y, Lin F, Xiong Z, Wu R, Zhang R, et al. Preparation and characterization of a collagen/chitosan/heparin matrix for an implantable bioartificial liver. J Biomater Sci Polym Ed 2005;16(9):1063-1080 (Pubitemid 44056344)
44. Wang XH, Li DP, Wang WJ, Feng QL, Cui FZ, Xu YX, et al. Crosslinked collagen/chitosan matrix for artificial livers. Biomaterials 2003;24(19):3213-3220 (Pubitemid 36577670)
45. Catapano G, De Bartolo L, Vico V, Ambrosio L. Morphology and metabolism of hepatocytes cultured in Petri dishes on films and in non-woven fabrics of hyaluronic acid esters. Biomaterials 2001;22(7):659-665 (Pubitemid 32112291)
46. Zavan B, Brun P, Vindigni V, Amadori A, Habeler W, Pontisso P, et al. Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies. Biomaterials 2005;26(34):7038-7045 (Pubitemid 41095526)
47. Li YS, Harn HJ, Hsieh DK, Wen TC, Subeq YM, Sun LY, et al. Cells and materials for liver tissue engineering. Cell Transpl 2013;22(4):685-700
48. Kumashiro Y, Fukumori K, Takahashi H, Nakayama M, Akiyama Y, Yamato M, et al. Modulation of cell adhesion and detachment on thermo-responsive polymeric surfaces through the observation of surface dynamics. Colloids Surf B 2013;106:198-207
49. Huang H, Hanada S, Kojima N, Sakai Y. Enhanced functional maturation of fetal porcine hepatocytes in three-dimensional poly-L-lactic acid scaffolds: a culture condition suitable for engineered liver tissues in large-scale animal studies. Cell Transpl 2006;15(8-9):799-809 (Pubitemid 46095262)
50. Jiang J, Kojima N, Guo L, Naruse K, Makuuchi M, Miyajima A, et al. Efficacy of engineered liver tissue based on poly-L-lactic acid scaffolds and fetal mouse liver cells cultured with oncostatin M, nicotinamide, and dimethyl sulfoxide. Tissue Eng 2004;10(9-10):1577-1586 (Pubitemid 39557861)
51. Mooney DJ, Sano K, Kaufmann PM, Majahod K, Schloo B, Vacanti JP, et al. Long-term engraftment of hepatocytes transplanted on biodegradable polymer sponges. J Biomed Mater Res 1997;37(3):413-420 (Pubitemid 27481456)
52. Ting Wang Z-QF, Leach MK, Wud JH, Jianga Q. Nanoporous fibers of type-I collagen coated poly(L-lactic acid) for enhancing primary hepatocyte growth and function. J Mater Chem B 2013;1:339-347
53. Torok E, Lutgehetmann M, Bierwolf J, Melbeck S, Dullmann J, Nashan B, et al. Primary human hepatocytes on biodegradable poly(L-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering. Liver Transpl 2011;17(2):104-114
54. Tamai M, Adachi E, Tagawa YI. Characterization of a liver organoid tissue composed of hepatocytes and fibroblasts in dense collagen fibrils. Tissue Eng A 2013;19(21-22):2527-2535
55. Otsuka H, Hirano A, Nagasaki Y, Okano T, Horiike Y, Kataoka K. Two-dimensional multiarray formation of hepatocyte spheroids on a microfabricated PEG-brush surface. ChemBioChem 2004;5(6):850-855 (Pubitemid 39257072)
56. Underhill GH, Chen AA, Albrecht DR, Bhatia SN. Assessment of hepatocellular function within PEG hydrogels. Biomaterials 2007;28(2):256-270 (Pubitemid 44537392)
57. Huang H, Oizumi S, Kojima N, Niino T, Sakai Y. Avidin-biotin binding-based cell seeding and perfusion culture of liver-derived cells in a porous scaffold with a three-dimensional interconnected flow-channel network. Biomaterials 2007;28(26):3815-3823 (Pubitemid 47048354)
58. Nettles DL, Chilkoti A, Setton LA. Applications of elastin-like polypeptides in tissue engineering. Adv Drug Deliv Rev 2010;62(15):1479-1485
59. Ohashi K, Yokoyama T, Yamato M, Kuge H, Kanehiro H, Tsutsumi M, et al. Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets. Nat Med 2007;13(7):880-885 (Pubitemid 47038184)
60. Tsuda Y, Kikuchi A, Yamato M, Nakao A, Sakurai Y, Umezu M, et al. The use of patterned dual thermoresponsive surfaces for the collective recovery as co-cultured cell sheets. Biomaterials 2005;26(14):1885-1893 (Pubitemid 39575330)
61. Kim M, Lee JY, Jones CN, Revzin A, Tae G. Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes. Biomaterials 2010;31(13):3596-3603
62. Baptista PM, Siddiqui MM, Lozier G, Rodriguez SR, Atala A, Soker S. The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 2011;53(2):604-617.
63. Uygun BE, Soto-Gutierrez A, Yagi H, Izamis ML, Guzzardi MA, Shulman C, et al. Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med 2010;16(7):814-820
64. Uygun BE, Yarmush ML. Engineered liver for transplantation. Curr Opin Biotechnol 2013;24(5):893-899
65. Du Y, Han R, Wen F, San Ng, San S, Xia L, Wohland T, et al. Synthetic sandwich culture of 3D hepatocyte monolayer. Biomaterials 2008;29(3):290-301 (Pubitemid 350081046)
66. Xia L, Sakban RB, Qu Y, Hong X, Zhang W, Nugraha B, et al. Tethered spheroids as an in vitro hepatocyte model for drug safety screening. Biomaterials 2012;33(7):2165-2176
67. Carlisle ES, Mariappan MR, Nelson KD, Thomes BE, Timmons RB, Constantinescu A, et al. Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling. Tissue Eng 2000;6(1):45-52 (Pubitemid 30123982)
68. Pricer WE Jr, Ashwell G. The binding of desialylated glycoproteins by plasma membranes of rat liver. J Biol Chem 1971;246(15):4825-4833
69. Weigel PH, Schmell E, Lee YC, Roseman S. Specific adhesion of rat hepatocytes to beta-galactosides linked to polyacrylamide gels. J Biol Chem 1978;253(2):330-333 (Pubitemid 8267648)
70. Cho CS, Seo SJ, Park IK, Kim SH, Kim TH, Hoshiba T, et al. Galactose-carrying polymers as extracellular matrices for liver tissue engineering. Biomaterials 2006;27(4):576-585 (Pubitemid 41464253)
71. Kim SH, Hoshiba T, Akaike T. Effect of carbohydrates attached to polystyrene on hepatocyte morphology on sugar-derivatized polystyrene matrices. J Biomed Mater Res A 2003;67(4):1351-1359 (Pubitemid 37522749)
72. Kobayashi A, Goto M, Kobayashi K, Akaike T. Receptor-mediated regulation of differentiation and proliferation of hepatocytes by synthetic polymer model of asialoglycoprotein. J Biomater Sci Polym Ed 1994;6(4):325-342
73. Lopina ST, Wu G, Merrill EW, Griffith-Cima L. Hepatocyte culture on carbohydrate-modified star polyethylene oxide hydrogels. Biomaterials 1996;17(6):559-569
74. Donati I, Gamini A, Vetere A, Campa C, Paoletti S. Synthesis, characterization, and preliminary biological study of glycoconjugates of poly(styrene-co-maleic acid). Biomacromolecules 2002;3(4):805-812
75. Yoon JJ, Nam YS, Kim JH, Park TG. Surface immobilization of galactose onto aliphatic biodegradable polymers for hepatocyte culture. Biotechnol Bioeng 2002;78(1):1-10 (Pubitemid 34712166)
76. Yin C, Ying L, Zhang PC, Zhuo RX, Kang ET, Leong KW, et al. High density of immobilized galactose ligand enhances hepatocyte attachment and function. J Biomed Mater Res A 2003;67(4):1093-1104 (Pubitemid 37522721)
77. Higashiyama S, Noda M, Kawase M, Yagi K. Mixed-ligand modification of polyamidoamine dendrimers to develop an effective scaffold for maintenance of hepatocyte spheroids. J Biomed Mater Res A 2003;64(3):475-482 (Pubitemid 37522487)
78. Turner R, Lozoya O, Wang Y, Cardinale V, Gaudio E, Alpini G, et al. Human hepatic stem cell and maturational liver lineage biology. Hepatology 2011;53(3):1035-1045
79. Semler EJ, Lancin PA, Dasgupta A, Moghe PV. Engineering hepatocellular morphogenesis and function via ligand-presenting hydrogels with graded mechanical compliance. Biotechnol Bioeng 2005;89(3):296-307 (Pubitemid 40288881)
80. You J, Park SA, Shin DS, Patel D, Raghunathan V, Kim M, et al. Characterizing the effects of heparin gel stiffness on function of primary hepatocytes. Tissue Eng Part A 2013;19(23-24):2655-2663
81. Ranucci CS, Kumar A, Batra SP, Moghe PV. Control of hepatocyte function on collagen foams: sizing matrix pores toward selective induction of 2-D and 3-D cellular morphogenesis. Biomaterials 2000;21(8):783-793 (Pubitemid 30216433)
82. Zmora S, Glicklis R, Cohen S. Tailoring the pore architecture in 3-D alginate scaffolds by controlling the freezing regime during fabrication. Biomaterials 2002;23(20):4087-4094 (Pubitemid 34765700)
83. Mishra R, Kumar A. Inorganic/organic biocomposite cryogels for regeneration of bony tissues. J Biomater Sci Polym Ed 2011;22(16):2107-2126
84. Bhat S, Tripathi A, Kumar A. Supermacroprous chitosan-agarose-gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering. J R Soc Interface 2011;8(57):540-554
85. Vishnoi T, Kumar A. Conducting cryogel scaffold as a potential biomaterial for cell stimulation and proliferation. J Mater Sci 2013;24(2):447-459
86. Langenbach R, Malick L, Tompa A, Kuszynski C, Freed H, Huberman E. Maintenance of adult rat hepatocytes on C3H/10T1/2 cells. Cancer Res 1979;39(9):3509-3514 (Pubitemid 9255774)
87. Guguen-Guillouzo C, Clement B, Baffet G, Beaumont C, Morel-Chany E, Glaise D, et al. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp Cell Res 1983;143(1):47-54 (Pubitemid 13145162)
88. Yamada M, Utoh R, Ohashi K, Tatsumi K, Yamato M, Okano T, et al. Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions. Biomaterials 2012;33(33):8304-8315
89. Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, et al. Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature 2013;499(7459):481-484
90. Kidambi S, Yarmush RS, Novik E, Chao P, Yarmush ML, Nahmias Y. Oxygen-mediated enhancement of primary hepatocyte metabolism, functional polarization, gene expression, and drug clearance. Proc Natl Acad Sci USA 2009;106(37):15714-15719
91. Simoni J. Artificial oxygen carriers: scientific and biotechnological points of view. Artif Organs 2009;33(2):92-96
92. Sullivan JP, Gordon JE, Palmer AF. Simulation of oxygen carrier mediated oxygen transport to C3A hepatoma cells housed within a hollow fiber bioreactor. Biotechnol Bioeng 2006;93(2):306-317
93. Matsui H, Osada T, Moroshita Y, Sekijima M, Fujii T, Takeuchi S, et al. Rapid and enhanced repolarization in sandwich-cultured hepatocytes on an oxygen-permeable membrane. Biochem Eng J 2010;52(2-3):255-262
94. Evenou F, Hamon M, Fujii T, Takeuchi S, Sakai Y. Gas-permeable membranes and co-culture with fibroblasts enable high-density hepatocyte culture as multilayered liver tissues. Biotechnol Prog 2011;27(4):1146-1153
95. Tilles AW, Berthiaume F, Yarmush ML, Tompkins RG, Toner M. Bioengineering of liver assist devices. J Hepatobiliary Pancreat Surg. 2002;9(6):686-696 (Pubitemid 36442934)
96. Park J, Berthiaume F, Toner M, Yarmush ML, Tilles AW. Microfabricated grooved substrates as platforms for bioartificial liver reactors. Biotechnol Bioeng 2005;90(5):632-644 (Pubitemid 40733992)
97. Lawrence BJ, Devarapalli M, Madihally SV. Flow dynamics in bioreactors containing tissue engineering scaffolds. Biotechnol Bioeng 2009;102(3):935-947
98. Leclerc E, Sakai Y, Fujii T. Microfluidic PDMS (polydimethylsiloxane) bioreactor for large-scale culture of hepatocytes. Biotechnol Prog 2004;20(3):750-755 (Pubitemid 38784012)
99. Hoque ME, Mao HQ, Ramakrishna S. Hybrid braided 3-D scaffold for bioartificial liver assist devices. J Biomater Sci Polym Ed 2007;18(1):45-58 (Pubitemid 46049856)
100. Lu JN, Wang CC, Young TH. The behaviors of long-term cryopreserved human hepatocytes on different biomaterials. Artif Organs 2011;35(3):E65-E72
101. Kim SS, Utsunomiya H, Koski JA, Wu BM, Cima MJ, Sohn J, et al. Survival and function of hepatocytes on a novel three-dimensional synthetic biodegradable polymer scaffold with an intrinsic network of channels. Ann Surg 1998;228(1):8-13 (Pubitemid 30190309)
102. Li J, Tao R, Wu W, Cao H, Xin J, Guo J, et al. 3D PLGA scaffolds improve differentiation and function of bone marrow mesenchymal stem cell-derived hepatocytes. Stem Cells Dev 2010;19(9):1427-1436
103. Tan GD, Toh GW, Birgersson E, Robens J, van Noort D, Leo HL. A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture. Biotechnol Bioeng 2013;110(6):1663-1673
104. Provin C, Takano K, Yoshida T, Sakai Y, Fujii T, Shirakashi R. Low O 2 metabolism of HepG2 cells cultured at high density in a 3D microstructured scaffold. Biomed Microdevices 2009;11(2):485-494
105. Soto-Gutierrez A, Kobayashi N, Rivas-Carrillo JD, Navarro-Alvarez N, Zhao D, Okitsu T, et al. Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes. Nat Biotechnol 2006;24(11):1412-1419 (Pubitemid 44722105)
106. Kazemnejad S, Allameh A, Soleimani M, Gharehbaghian A, Mohammadi Y, Amirizadeh N, et al. Biochemical and molecular characterization of hepatocyte-like cells derived from human bone marrow mesenchymal stem cells on a novel three-dimensional biocompatible nanofibrous scaffold. J Gastroenterol Hepatol 2009;24(2):278-287
107. Tsang VL, Chen AA, Cho LM, Jadin KD, Sah RL, DeLong S, et al. Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels. FASEB J 2007;21(3):790-801 (Pubitemid 46348254)
108. Scott EA, Nichols MD, Kuntz-Willits R, Elbert DL. Modular scaffolds assembled around living cells using poly(ethylene glycol) microspheres with macroporation via a non-cytotoxic porogen. Acta Biomater 2010;6(1):29-38
109. Lee J, Cuddihy MJ, Cater GM, Kotov NA. Engineering liver tissue spheroids with inverted colloidal crystal scaffolds. Biomaterials 2009;30(27):4687-4694
110. Bruns H, Kneser U, Holzhuter S, Roth B, Kluth J, Kaufmann PM, et al. Injectable liver: a novel approach using fibrin gel as a matrix for culture and intrahepatic transplantation of hepatocytes. Tissue Eng 2005;11(11-12):1718-1726 (Pubitemid 43120183)
111. Orkin RW, Gehron P, McGoodwin EB, Martin GR, Valentine T, Swarm R. A murine tumor producing a matrix of basement membrane. J Exp Med 1977;145(1):204-220 (Pubitemid 8010101)
112. Schuetz EG, Li D, Omiecinski CJ, Muller-Eberhard U, Kleinman HK, Elswick B, et al. Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix. J Cell Physiol 1988;134(3):309-323 (Pubitemid 18092266)
113. Bierwolf J, Lutgehetmann M, Feng K, Erbes J, Deichmann S, Toronyi E, et al. Primary rat hepatocyte culture on 3D nanofibrous polymer scaffolds for toxicology and pharmaceutical research. Biotechnol Bioeng 2011;108(1):141-150
114. Sosnik A, Sefton MV. Semi-synthetic collagen/poloxamine matrices for tissue engineering. Biomaterials 2005;26(35):7425-7435 (Pubitemid 41133594)
115. Miranda JP, Leite SB, Muller-Vieira U, Rodrigues A, Carrondo MJ, Alves PM. Towards an extended functional hepatocyte in vitro culture. Tissue Eng C 2009;15(2):157-167
116. Schmelzer E, Triolo F, Turner ME, Thompson RL, Zeilinger K, Reid LM, et al. Three-dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells. Tissue Eng A 2010;16(6):2007-2016
117. Turner WS, Schmelzer E, McClelland R, Wauthier E, Chen W, Reid LM. Human hepatoblast phenotype maintained by hyaluronan hydrogels. J Biomed Mater Res B 2007;82(1):156-168 (Pubitemid 47015365)