Hepatocyte behavior within three-dimensional porous alginate scaffolds

Biotechnology and Bioengineering

Volumn 67, Issue 3, 2000, Pages 344-353

  Glicklis, Rachel ^a   Shapiro, Lilia ^a   Agbaria, Riad ^a   Merchuk, Jose C ^a   Cohen, Smadar ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

3 D hepatocyte culture; Alginate scaffolds; Hepatocyte aggregation; Tissue engineering

Indexed keywords

AGGLOMERATION; ALGAE; CELL CULTURE; CELLS; COLLAGEN; PHYSIOLOGICAL MODELS; PORE SIZE; POROUS MATERIALS; PROTEINS;

ALGINATE; HEPATOCYTES;

BIOTECHNOLOGY;

ALGINIC ACID; DNA;

ANIMAL CELL; ARTICLE; CELL AGGREGATION; CELL COUNT; CELL CULTURE; LIVER CELL CULTURE; MALE; NONHUMAN; RAT; SPHEROID CELL; THREE DIMENSIONAL IMAGING;

ALBUMINS; ALGINATES; ANIMALS; BIOTECHNOLOGY; CELL AGGREGATION; CELL AGING; CELL CULTURE TECHNIQUES; CELL SIZE; CELL SURVIVAL; CELLS, CULTURED; COLLAGEN; FIBRONECTINS; GLUCURONIC ACID; HEXURONIC ACIDS; LIVER; LIVER, ARTIFICIAL; MALE; MEMBRANES, ARTIFICIAL; MICROSCOPY, ELECTRON, SCANNING; RATS; RATS, SPRAGUE-DAWLEY; UREA;

ALGAE; ANIMALIA;

EID: 0034606915     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000205)67:3<344::AID-BIT11>3.0.CO;2-2     Document Type: Article

References (34)

1. Asonuma K, Gilbert JC, Stein JE, Takeda T, Vacanti JP. 1992. Quantitation of transplanted hepatic mass necessary to cure the Gunn rat model of hyperbilirubunemia. J Pediatr Surg 27:298-301.
2. Bader A, Borel Rinkes IH, Closs EI, Ryan CM, Toner M, Cunningham JM, Tomkins RG, Yarmush ML. 1992. A stable long-term hepatocyte culture system for studies of physiologic processes: cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotechnol Prog 8:219-225.
3. Berry MN, Friend DS. 1969. High yield preparation of isolated rat parenchymal cells. A biochemical and fine structure study. J Cell Biol 43:506-520.
4. Bissell DM, Caron JM, Babiss LE, Friedman JM. 1990. Transcriptional regulation of the albumin gene in cultured rat hepatocytes. Role of basement-membrane matrix. Mol Biol Med 7:187-197.
5. Brunk CF, Jones KC, James TW. 1979. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 92:497-700.
6. Chaney AL, Marback EP. 1962. Modified reagents for determination of urea and ammonia. Clin Chem 8:130-132.
7. Coger R, Toner M, Moghe P, Ezzell RM, Yarmush ML. 1997. Hepatocyte aggregation and reorganization of EHS matrix gel. Tissue Eng 3: 375-390.
8. Dunn JCY, Tomkins RG, Yarmush ML. 1991. Long-term in vitro functions of adult hepatocytes in a collagen sandwich configuration. Biotechnol Prog 7:237-242.
9. Grant GT, Morris ER, Rees DA, Smith PJC, Thom D. 1973. Biological interactions between polysaccharides and divalent cations: the egg box model. FEBS Lett 32:195-198.
10. Ijima H, Matsushita T, Nakazawa K, Fujii Y, Funatsu K. 1998. Hepatocyte spheroids in polyurethane foams: functional analysis and application for a hybrid artificial liver. Tissue Eng 4:213-226.
11. Johansson S, Hook M. 1984. Substrate adhesion of rat hepatocytes: on the mechanism of attachment to fibronectin. J Cell Biol 98:810-817.
12. Kaufmann PM, Heimrath S, Kim BS, Mooney DJ. 1997. Highly porous polymer matrices as a three-dimensional culture system for hepatocytes. Cell Transplantation 6:463-468.
13. Klöck G, Frank H, Houben R, Zekorn T, Horcher A, Siebers U, Wöhrle M, Federin K, Zimmermann U. 1994. Production of purified alginates suitable for use in immunoisolated transplantation. Appl Microbiol Biotechnol 40:638-643.
14. Koide N, Sakaguchi K, Koide Y, et al. 1990. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp Cell Res 186:227-235.
15. Kreamer BL, Staecker JL, Sawada N, Sattler GL, Hsia MTS, Pitot HC. 1986. Use of low speed, iso-density Percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. In Vitro Cell Dev Biol 22:201-205.
16. Kulseng B, Skjåk-Braek G, Ryan L, Andersson A, King A, Faxvaag A, Espevik T. 1999. Transplantation of alginate capsules. Transplantation 67:978-984.
17. Landry J, Bernier C, Ouellet C, Goyette R, Marceau N. 1985. Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J Cell Biol 101: 914-923.
18. Langer R, Vacanti JP. 1993. Tissue engineering. Science 260:920-926.
19. Lanza RP, Kühtreiber WM, Ecker D, Staruk JE, Chick WL. 1995. Xenotransplantation of porcine and bovine islets without immunosuppression using uncoated alginate microspheres. Transplantation 59: 1377-1384.
20. Lin KH, Maeda S, Saito T. 1995. Long-term maintenance of liver-specific functions in three-dimensional culture of adult rat hepatocytes with a porous gelatin sponge support. Biotechnol Appl Biochem 21:19-27.
21. Mikos AG, Bao Y, Cima LG, Ingber DE, Vacanti JP, Langer R. 1993. Preparation of poly(glycolic acid) bonded fiber structures for cell attachment and transplantation. J Biomed Mater Res 27:183-189.
22. Mikos AG, Thorsen AJ, Czerwonka LA, Bao Y, Winslow DN, Vacanti JP, Langer R. 1994. Preparation and characterization of poly(L-lactic acid) foams. Polymer 35:1068-1077.
23. Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. 1996. Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials 17:373-385.
24. Nehrer S, Breinan HA, Ramappa A, Young G, Shortkroff S, Louie LK, Sledge CB, Yannas IV, Spector M. 1997. Matrix collagen type and pore size influence behaviour of seeded canine chondrocytes. Biomaterials 18:769-776.
25. Peshwa MV, Wu FJ, Sharp HS, Cerra FB, Hu WS. 1994. Kinetics of hepatocyte spheroid formation. Biotechnol Prog 10:460-466.
26. Peter TJ, Peter JC. 1972. The biosynthesis of rat albumin serum. J Biol Chem 247:3858-3864.
27. Powers MJ, Griffith-Cima L. 1996. Motility behavior of hepatocytes on extracellular matrix during aggregation. Tissue Eng 50:392-403.
28. Rojkind M, Greenwel P. 1994. The extracellular matrix of the liver. In: Arias IM, Boyer JL, Fausto N, Jakoby WB, Schachter D, Shafritz DA, editors. The liver: Biology and pathobiology. New York: Raven Press. p 843-868.
29. Schwerer B, Bach M, Bernheimer H. 1987. ELISA for determination of albumin in the nanogram range: Assay in comparison with radial immunodiffusion. Clin Chem Acta 163:237-244.
30. Shapiro L, Cohen S. 1997. Novel alginate sponges for cell culture and transplantation. Biomatenals 18:581-590.
31. Stockmann HBA, Tompkins RG, Berthiaume F. 1997. Expression of long-term liver specific function by adult rat hepatocytes cultured on microcarriers. Tissue Eng 3:267-279.
32. Wagner WR, Muzzio DJ, Rilo HR, Deglau T, Ataai MM, Michalopoulos GK, Block GD. 1997. Effect of growth factors and defined medium on primary hepatocyte culture on polyester carriers with varying surface treatment. Tissue Eng 3:289-301.
33. Yagi K, Tsuda K, Serada M, Yamada C, Kondoh AM, Miura Y. 1993. Rapid formation of multicellular spheroids of adult rat hepatocytes by rotation culture and their immobilization within calcium alginate. Artif Organs 17:929-934.
34. Yuasa C, Tomita Y, Shono M, Ishimura K, Ishihara A. 1993. Importance of cell aggregation for expression of liver functions and regeneration demonstrated with primary cultured hepatocytes. J Cell Physiol 156: 522-530.