A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture

Biotechnology and Bioengineering

Volumn 110, Issue 6, 2013, Pages 1663-1673

  Tan, Guo Dong Sean ^a   Toh, Guoyang William ^a   Birgersson, Erik ^a   Robens, Jeffrey ^b   van Noort, Danny ^b   Leo, Hwa Liang ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Diffusion; Direct oxygenation; Mass transport; Oxygen concentration; Shear stress

Indexed keywords

LIVER-SPECIFIC FUNCTIONS; LONG-TERM MAINTENANCES; OXYGEN CONCENTRATIONS; PERMEABLE MATERIAL; POLYDIMETHYLSILOXANE PDMS; SANDWICH CONFIGURATION; SEEDING DENSITY; SYNERGISTIC EFFECT;

BIOCONVERSION; BIOREACTORS; CELLS; COMPUTATIONAL FLUID DYNAMICS; CYTOLOGY; DIFFUSION; FLOW MEASUREMENT; MAINTENANCE; MASS TRANSFER; MICROCHANNELS; OXYGEN; OXYGENATION; SHEAR STRESS;

SILICONES;

DIMETICONE;

ANIMAL CELL; ARTICLE; CELL CONTACT; CELL DENSITY; COMPUTATIONAL FLUID DYNAMICS; CONTROLLED STUDY; DRUG SCREENING; LIVER CELL; LIVER CELL CULTURE; NONHUMAN; SHEAR STRESS;

ALBUMINS; ANALYSIS OF VARIANCE; ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; DIFFUSION; DIMETHYLPOLYSILOXANES; EQUIPMENT DESIGN; HEPATOCYTES; MALE; MICROFLUIDICS; OXYGEN; RATS; RATS, WISTAR; TOXICITY TESTS;

EID: 84876780202     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24822     Document Type: Article

References (36)

1. Balis UJ, Behnia K, Dwarakanath B, Bhatia SN, Sullivan SJ, Yarmush ML, Toner M. 1999. Oxygen consumption characteristics of porcine hepatocytes. Metab Eng 1(1): 49-62.
2. Berthiaume F, Moghe PV, Toner M, Yarmush ML. 1996. Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: Hepatocytes cultured in a sandwich configuration. FASEB J 10(13): 1471-1484.
3. Bissell DM. 1981. Primary hepatocyte culture: Substratum requirements and production of matrix components. Fed Proc 40(10): 2469-2473.
4. Consolo F, Fiore GB, Truscello S, Caronna M, Morbiducci U, Montevecchi FM, Redaelli A. 2009. A computational model for the optimization of transport phenomena in a rotating hollow-fiber bioreactor for artificial liver. Tissue Eng C 15(1): 41-55.
5. Cox ME, Dunn B. 1986. Oxygen diffusion in poly(dimethyl siloxane) using fluorescence Quenching .2. Filled samples. J Polym Sc Part a-Polym Chemi 24(10): 2395-2400.
6. Evenou F, Fujii T, Sakai Y. 2010. Spontaneous formation of stably-attached and 3D-organized hepatocyte aggregates on oxygen-permeable polydimethylsiloxane membranes having 3D microstructures. Biomed Microdevices 12(3): 465-475.
7. Foy BD, Rotem A, Toner M, Tompkins RG, Yarmush ML. 1994. A device to measure the oxygen uptake rate of attached cells: Importance in bioartificial organ design. Cell Transplant 3(6): 515-527.
8. Godoy P, Hengstler JG, Ilkavets I, Meyer C, Bachmann A, Muller A, Tuschl G, Mueller SO, Dooley S. 2009. Extracellular matrix modulates sensitivity of hepatocytes to fibroblastoid dedifferentiation and transforming growth factor beta-induced apoptosis. Hepatology 49(6): 2031-2043.
9. Guillouzo A. 1998. Liver cell models in in vitro toxicology. Environ Health Perspect 106(Suppl 2): 511-532.
10. Hamon M, Hanada S, Fujii T, Sakai Y. 2012. Direct oxygen supply with polydimethylsiloxane (PDMS) membranes induces a spontaneous organization of thick heterogeneous liver tissues from rat fetal liver cells in vitro. Cell Transplant 21(2-3): 401-410.
11. Hay PD, Veitch AR, Gaylor JD. 2001. Oxygen transfer in a convection-enhanced hollow fiber bioartificial liver. Artif Organs 25(2): 119-130.
12. Kan P, Miyoshi H, Yanagi K, Ohshima N. 1998. Effects of shear stress on metabolic function of the co-culture system of hepatocyte/nonparenchymal cells for a bioartificial liver. ASAIO J 44(5): M441-M444.
13. Kane BJ, Zinner MJ, Yarmush ML, Toner M. 2006. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes. Anal Chem 78(13): 4291-4298.
14. Kidambi S, Yarmush RS, Novik E, Chao P, Yarmush ML, Nahmias Y. 2009. Oxygen-mediated enhancement of primary hepatocyte metabolism, functional polarization, gene expression, and drug clearance. Proc Natl Acad Sci USA 106(37): 15714-15719.
15. Kim SS, Sundback CA, Kaihara S, Benvenuto MS, Kim BS, Mooney DJ, Vacanti JP. 2000. Dynamic seeding and in vitro culture of hepatocytes in a flow perfusion system. Tissue Eng 6(1): 39-44.
16. Leclerc E, Sakai Y, Fujii T. 2004. Microfluidic PDMS (polydimethylsiloxane) bioreactor for large-scale culture of hepatocytes. Biotechnol Prog 20(3): 750-755.
17. LeCluyse EL, Audus KL, Hochman JH. 1994. Formation of extensive canalicular networks by rat hepatocytes cultured in collagen-sandwich configuration. Am J Physiol 266(6 Pt 1): C1764-C1774.
18. Lieberthal W, Wolf EF, Merrill EW, Levinsky NG, Valeri CR. 1987. Hemodynamic effects of different preparations of stroma free hemolysates in the isolated perfused rat kidney. Life Sci 41(23): 2525-2533.
19. Lin SH. 1976. Oxygen diffusion in a spherical cell with nonlinear oxygen uptake kinetics. J Theor Biol 60(2): 449-457.
20. Matsui H, Osada T, Moroshita Y, Sekijima M, Fujii T, Takeuchi S, Sakai Y. 2010. Rapid and enhanced repolarization in sandwich-cultured hepatocytes on an oxygen-permeable membrane. Biochem Eng J 52(2-3): 255-262.
21. Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. 1996. Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials 17(3): 373-385.
22. Moghe PV, Coger RN, Toner M, Yarmush ML. 1997. Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel. Biotechnol Bioeng 56(6): 706-711.
23. Nahmias Y, Kramvis Y, Barbe L, Casali M, Berthiaume F, Yarmush ML. 2006. A novel formulation of oxygen-carrying matrix enhances liver-specific function of cultured hepatocytes. FASEB J 20(14): 2531-2533.
24. Nahmias Y, Berthiaume F, Yarmush ML. 2007. Integration of technologies for hepatic tissue engineering. Adv Biochem Eng Biotechnol 103: 309-329.
25. Nishikawa M, Kojima N, Komori K, Yamamoto T, Fujii T, Sakai Y. 2008. Enhanced maintenance and functions of rat hepatocytes induced by combination of on-site oxygenation and coculture with fibroblasts. J Biotechnol 133(2): 253-260.
26. Nyberg G, Fager G, Mjornstedt L, Olausson M. 1991. Metabolic risk factors for cardiovascular disease in pancreas and kidney transplant recipients. Diabetologia 34(Suppl 1): S44-S46.
27. Ostrovidov S, Jiang J, Sakai Y, Fujii T. 2004. Membrane-based PDMS microbioreactor for perfused 3D primary rat hepatocyte cultures. Biomed Microdevices 6(4): 279-287.
28. Rotem A, Toner M, Tompkins RG, Yarmush ML. 1992. Oxygen uptake rates in cultured rat hepatocytes. Biotechnol Bioeng 40(10): 1286-1291.
29. Seglen PO. 1976. Preparation of isolated rat liver cells. Methods Cell Biol 13: 29-83.
30. Tilles AW, Baskaran H, Roy P, Yarmush ML, Toner M. 2001. Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor. Biotechnol Bioeng 73(5): 379-389.
31. Xia L, Ng S, Han R, Tuo X, Xiao G, Leo HL, Cheng T, Yu H. 2009. Laminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing. Biomaterials 30(30): 5927-5936.
32. Xu JJ, Diaz D, O'Brien PJ. 2004. Applications of cytotoxicity assays and pre-lethal mechanistic assays for assessment of human hepatotoxicity potential. Chem Biol Interact 150(1): 115-128.
33. Yanagi K, Ohshima N. 2001. Improvement of metabolic performance of cultured hepatocytes by high oxygen tension in the atmosphere. Artif Organs 25(1): 1-6.
34. Yarmush ML, Toner M, Dunn JC, Rotem A, Hubel A, Tompkins RG. 1992. Hepatic tissue engineering. Development of critical technologies. Ann N Y Acad Sci 665: 238-252.
35. Zhang MY, Lee PJ, Hung PJ, Johnson T, Lee LP, Mofrad MR. 2008. Microfluidic environment for high density hepatocyte culture. Biomed Microdevices 10(1): 117-121.
36. Zhang S, Tong W, Zheng B, Susanto TA, Xia L, Zhang C, Ananthanarayanan A, Tuo X, Sakban RB, Jia R, et al. 2011. A robust high-throughput sandwich cell-based drug screening platform. Biomaterials 32(4): 1229-1241.