Storage stability of optimal liposome-polyethylenimine complexes (lipopolyplexes) for DNA or siRNA delivery

Acta Biomaterialia

Volumn 10, Issue 6, 2014, Pages 2663-2673

  Ewe, Alexander ^a   Schaper, Andreas ^b   Barnert, Sabine ^c   Schubert, Rolf ^c   Temme, Achim ^d   Bakowsky, Udo ^b   Aigner, Achim ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c UNIVERSITY OF FREIBURG   (Germany)

^d UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

Author keywords

Lipopolyplexes; Liposomes; PEI; Polyethylenimine; siRNA

Indexed keywords

BIOACTIVITY; BIOCOMPATIBILITY; BIOMOLECULES; DNA; GENE TRANSFER; GENES; STABILITY;

CATIONIC POLYMERS; DNA DELIVERY; IN-VIVO; LIPOPOLYPLEXES; POLY(ETHYLENIMINE); SIRNA; SIRNA DELIVERY; STORAGE STABILITY; THERAPEUTIC APPLICATION;

LIPOSOMES;

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; BETA GALACTOSIDASE; DNA; LIPOSOME; NANOPARTICLE; POLYETHYLENEIMINE; SMALL INTERFERING RNA;

ARTICLE; BIOCOMPATIBILITY; BIOLOGICAL ACTIVITY; CELL VIABILITY; COMPLEX FORMATION; CRYOELECTRON MICROSCOPY; CYTOTOXICITY; DNA TRANSFECTION; MOLECULAR WEIGHT; NONVIRAL GENE DELIVERY SYSTEM; PARTICLE SIZE; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; STORAGE TEMPERATURE; SURFACE CHARGE; TRANSMISSION ELECTRON MICROSCOPY; ZETA POTENTIAL; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CRYOELECTRON MICROSCOPY; DNA; LIPOSOMES; MICROSCOPY, ELECTRON, TRANSMISSION; POLYETHYLENEIMINE; RNA, SMALL INTERFERING;

EID: 84899632454     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2014.02.037     Document Type: Article

References (66)

1. Kay MA. State-of-the-art gene-based therapies: the road ahead. Nat Rev Genet 2011;12:316-28.
2. Itaka K, Kataoka K. Recent development of nonviral gene delivery systems with virus-like structures and mechanisms. Eur J Pharm Biopharm 2009;71:475-83.
3. Piskin E, Dincer S, Turk M. Gene delivery: intelligent but just at the beginning. J Biomater Sci 2004;15:1181-202.
4. Basarkar A, Singh J. Nanoparticulate systems for polynucleotide delivery. Int J Nanomed 2007;2:353-60.
5. Aigner A. Cellular delivery in vivo of siRNA-based therapeutics. Curr Pharm Des 2008;14:3603-19.
6. Kodama K, Katayama Y, Shoji Y, Nakashima H. The features and shortcomings for gene delivery of current non-viral carriers. Curr Med Chem 2006;13:2155-61.
7. Ravi Kumar MN, Sameti M, Mohapatra SS, Kong X, Lockey RF, Bakowsky U, et al. Cationic silica nanoparticles as gene carriers: synthesis, characterization and transfection efficiency in vitro and in vivo. J Nanosci Nanotechnol 2004;4:876-81.
8. Kneuer C, Ehrhardt C, Bakowsky H, Kumar MN, Oberle V, Lehr CM, et al. The influence of physicochemical parameters on the efficacy of non-viral DNA transfection complexes: a comparative study. J Nanosci Nanotechnol 2006;6:2776-82.
9. Xu Y, Szoka Jr FC. Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection. Biochemistry 1996;35:5616-23.
10. Pelisek J, Gaedtke L, DeRouchey J, Walker GF, Nikol S, Wagner E. Optimized lipopolyplex formulations for gene transfer to human colon carcinoma cells under in vitro conditions. J Gene Med 2006;8:186-97.
11. Semple SC, Akinc A, Chen J, Sandhu AP, Mui BL, Cho CK, et al. Rational design of cationic lipids for siRNA delivery. Nat Biotechnol 2010;28:172-6.
12. Balazs DA, Godbey W. Liposomes for use in gene delivery. J Drug Deliv 2011;2011:326497.
13. Zhang S, Xu Y, Wang B, Qiao W, Liu D, Li Z. Cationic compounds used in lipoplexes and polyplexes for gene delivery. J Controlled Release 2004;100:165-80.
14. Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci USA 1995;92:7297-301.
15. Neu M, Fischer D, Kissel T. Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives. J Gene Med 2005;7:992-1009.
16. Lai WF. In vivo nucleic acid delivery with PEI and its derivatives: current status and perspectives. Exp Rev Med Dev 2011;8:173-85.
17. Hobel S, Aigner A. Polyethylenimines for siRNA and miRNA delivery in vivo. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2013.
18. Godbey WT, Wu KK, Mikos AG. Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. J Biomed Mater Res 1999;45:268-75.
19. Tang MX, Szoka FC. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther 1997;4:823-32.
20. Lungwitz U, Breunig M, Blunk T, Gopferich A. Polyethylenimine-based nonviral gene delivery systems. Eur J Pharm Biopharm 2005;60:247-66.
21. Zuber G, Dauty E, Nothisen M, Belguise P, Behr JP. Towards synthetic viruses. Adv Drug Deliv Rev 2001;52:245-53.
22. Behr JP. The proton sponge: a trick to enter cells the viruses did not exploit. Chimia 1997;51:34-6.
23. Godbey WT, Wu KK, Mikos AG. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci USA 1999;96:5177-81.
24. Pollard H, Remy JS, Loussouarn G, Demolombe S, Behr JP, Escande D. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J Biol Chem 1998;273:7507-11.
25. Werth S, Urban-Klein B, Dai L, Hobel S, Grzelinski M, Bakowsky U, et al. A low molecular weight fraction of polyethylenimine (PEI) displays increased transfection efficiency of DNA and siRNA in fresh or lyophilized complexes. J Controlled Release 2006;112:257-70.
26. Kwok A, Hart SL. Comparative structural and functional studies of nanoparticle formulations for DNA and siRNA delivery. Nanomed Nanotechnol Biol Med 2011;7:210-9.
27. Wiseman JW, Goddard CA, McLelland D, Colledge WH. A comparison of linear and branched polyethylenimine (PEI) with DCChol/DOPE liposomes for gene delivery to epithelial cells in vitro and in vivo. Gene Ther 2003;10:1654-62.
28. Malek A, Czubayko F, Aigner A. PEG grafting of polyethylenimine (PEI) exerts different effects on DNA transfection and siRNA-induced gene targeting efficacy. J Drug Target 2008;16:124-39.
29. Mao S, Neu M, Germershaus O, Merkel O, Sitterberg J, Bakowsky U, et al. Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly(ethylene imine)-graft-poly(ethylene glycol) block copolymer/SiRNA polyplexes. Bioconjugate Chem 2006;17:1209-18.
30. Nguyen HK, Lemieux P, Vinogradov SV, Gebhart CL, Guerin N, Paradis G, et al. Evaluation of polyether-polyethyleneimine graft copolymers as gene transfer agents. Gene Ther 2000;7:126-38.
31. Erbacher P, Bettinger T, Belguise-Valladier P, Zou S, Coll JL, Behr JP, et al. Transfection and physical properties of various saccharide, poly(ethylene glycol), and antibody-derivatized polyethylenimines (PEI). J Gene Med 1999;1:210-22.
32. Bauhuber S, Liebl R, Tomasetti L, Rachel R, Goepferich A, Breunig M. A library of strictly linear poly(ethylene glycol)-poly(ethylene imine) diblock copolymers to perform structure-function relationship of non-viral gene carriers. J Controlled Release 2012;162:446-55.
33. Hobel S, Loos A, Appelhans D, Schwarz S, Seidel J, Voit B, et al. Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): physicochemical and biological properties of DNA and siRNA complexes. J Controlled Release 2011;149:146-58.
34. Jiang HL, Kim TH, Kim YK, Park IY, Cho MH, Cho CS. Efficient gene delivery using chitosan-polyethylenimine hybrid systems. Biomed Mater (Bristol, England) 2008;3:25013.
35. Ito T, Yoshihara C, Hamada K, Koyama Y. DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice. Biomaterials 2010;31:2912-8.
36. Zhang L, Hu CH, Cheng SX, Zhuo RX. PEI grafted hyperbranched polymers with polyglycerol as a core for gene delivery. Colloids Surf 2010;76:427-33.
37. Noga M, Edinger D, Rodl W, Wagner E, Winter G, Besheer A. Controlled shielding and deshielding of gene delivery polyplexes using hydroxyethyl starch (HES) and alpha-amylase. J Controlled Release 2012;159:92-103.
38. Schroeder A, Dahlman JE, Sahay G, Love KT, Jiang S, Eltoukhy AA, et al. Alkanemodified short polyethyleneimine for siRNA delivery. J Controlled Release 2012;160:172-6.
39. Fortune JA, Novobrantseva TI, Klibanov AM. Highly effective gene transfection in vivo by alkylated polyethylenimine. J Drug Deliv 2011;2011:204058.
40. Oskuee RK, Dehshahri A, Shier WT, Ramezani M. Alkylcarboxylate grafting to polyethylenimine: a simple approach to producing a DNA nanocarrier with low toxicity. J Gene Med 2009;11:921-32.
41. Jiang D, Salem AK. Optimized dextran-polyethylenimine conjugates are efficient non-viral vectors with reduced cytotoxicity when used in serum containing environments. Int J Pharm 2012;427:71-9.
42. Tripathi SK, Goyal R, Kumar P, Gupta KC. Linear polyethylenimine- graftchitosan copolymers as efficient DNA/siRNA delivery vectors in vitro and in vivo. Nanomed Nanotechnol Biol Med 2012;8:337-45.
43. Wang X, Yao J, Zhou JP, Lu Y, Wang W. Synthesis and evaluation of chitosangraft- polyethylenimine as a gene vector. Die Pharm 2010;65:572-9.
44. Hsu CY, Hendzel M, Uludag H. Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell. J Gene Med 2011;13:46-59.
45. Alshamsan A, Haddadi A, Incani V, Samuel J, Lavasanifar A, Uludag H. Formulation and delivery of siRNA by oleic acid and stearic acid modified polyethylenimine. Mol Pharm 2009;6:121-33.
46. Aldawsari H, Edrada-Ebel R, Blatchford DR, Tate RJ, Tetley L, Dufes C. Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polypropylenimine polyplex. Biomaterials 2011;32:5889-99.
47. Garcia L, Bunuales M, Duzgunes N, Tros de Ilarduya C. Serum-resistant lipopolyplexes for gene delivery to liver tumour cells. Eur J Pharm Biopharm 2007;67:58-66.
48. Lee CH, Ni YH, Chen CC, Chou C, Chang FH. Synergistic effect of polyethylenimine and cationic liposomes in nucleic acid delivery to human cancer cells. Biochim Biophys Acta 2003;1611:55-62.
49. Gaedtke L, Pelisek J, Lipinski KS, Wrighton CJ, Wagner E. Transcriptionally targeted nonviral gene transfer using a beta-catenin/TCF- dependent promoter in a series of different human low passage colon cancer cells. Mol Pharm 2007;4:129-39.
50. Hanzlikova M, Soininen P, Lampela P, Mannisto PT, Raasmaja A. The role of PEI structure and size in the PEI/liposome-mediated synergism of gene transfection. Plasmid 2009;61:15-21.
51. Schafer J, Hobel S, Bakowsky U, Aigner A. Liposome-polyethylenimine complexes for enhanced DNA and siRNA delivery. Biomaterials 2010.
52. Heyes J, Palmer L, Chan K, Giesbrecht C, Jeffs L, MacLachlan I. Lipid encapsulation enables the effective systemic delivery of polyplex plasmid DNA. Mol Ther 2007;15:713-20.
53. Ko YT, Bhattacharya R, Bickel U. Liposome encapsulated polyethylenimine/ ODN polyplexes for brain targeting. J Controlled Release 2009;133:230-7.
54. Egle R, Milek M, Mlinaric-Rascan I, Fahr A, Kristl J. A novel gene delivery system for stable transfection of thiopurine-S-methyltransferase gene in versatile cell types. Eur J Pharm Biopharm 2008;69:23-30.
55. Navarro G, Sawant RR, Essex S, Tros de Ilarduya C, Torchilin VP. Phospholipid- polyethylenimine conjugate-based micelle-like nanoparticles for siRNA delivery. Drug Deliv Transl Res 2011;1:25-33.
56. Hobel S, Koburger I, John M, Czubayko F, Hadwiger P, Vornlocher HP, et al. Polyethylenimine/small interfering RNA-mediated knockdown of vascular endothelial growth factor in vivo exerts anti-tumor effects synergistically with Bevacizumab. J Gene Med 2010;12:287-300.
57. Hobel S, Prinz R, Malek A, Urban-Klein B, Sitterberg J, Bakowsky U, et al. Polyethylenimine PEI F25-LMW allows the long-term storage of frozen complexes as fully active reagents in siRNA-mediated gene targeting and DNA delivery. Eur J Pharm Biopharm 2008;70:29-41.
58. Ibrahim AF, Weirauch U, Thomas M, Grunweller A, Hartmann RK, Aigner A. MicroRNA replacement therapy for miR-145 and miR-33a is efficacious in a model of colon carcinoma. Cancer Res 2011;71:5214-24.
59. Holzer M, Barnert S, Momm J, Schubert R. Preparative size exclusion chromatography combined with detergent removal as a versatile tool to prepare unilamellar and spherical liposomes of highly uniform size distribution. J Chromatogr A 2009;1216:5838-48.
60. Movassaghian S, Moghimi HR, Shirazi FH, Koshkaryev A, Trivedi MS, Torchilin VP. Efficient down-regulation of PKC-alpha gene expression in A549 lung cancer cells mediated by antisense oligodeoxynucleotides in dendrosomes. Int J Pharm 2013;441:82-91.
61. Zintchenko A, Konak C. Interaction of DNA/polycation complexes with phospholipids: stabilizing strategy for gene delivery. Macromol Biosci 2005;5:1169-74.
62. Opanasopit P, Paecharoenchai O, Rojanarata T, Ngawhirunpat T, Ruktanonchai U. Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes. Int J Nanomed 2011;6:975-83.
63. Brus C, Kleemann E, Aigner A, Czubayko F, Kissel T. Stabilization of oligonucleotide-polyethylenimine complexes by freeze-drying: physicochemical and biological characterization. J Controlled Release 2004;95:119-31.
64. Tros de Ilarduya C, Garcia L, Duzgunes N. Liposomes and lipopolymeric carriers for gene delivery. J Microencapsul 2010;27:602-8.
65. Guo W, Lee RJ. Efficient gene delivery using anionic liposome-complexed polyplexes (LPDII). Biosci Rep 2000;20:419-32.
66. Luo X, Feng M, Pan S, Wen Y, Zhang W, Wu C. Charge shielding effects on gene delivery of polyethylenimine/DNA complexes: PEGylation and phospholipid coating. J Mater Sci Mater Med 2012;23:1685-95.