Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell

Journal of Gene Medicine

Volumn 13, Issue 1, 2011, Pages 46-59

  Hsu, Charlie Yu Ming ^a   Hendzel, Michael ^a   Uludaǧ, Hasan ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Bone marrow; Flow cytometry; Gene transfer; Nonviral vector; Nuclear association; Nuclei extraction

Indexed keywords

GREEN FLUORESCENT PROTEIN; LIPID; PLASMID DNA; POLYETHYLENEIMINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE MARROW CELL; CELL FRACTIONATION; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC TRANSFECTION; HETEROZYGOTE; INTRACELLULAR TRANSPORT; KINETICS; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STROMA CELL; TRANSGENE;

ANIMALS; BONE MARROW CELLS; CATIONS; CELL NUCLEUS; CELLS, CULTURED; DNA; FEMALE; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LIPIDS; PLASMIDS; POLYETHYLENEIMINE; POLYMERS; RATS; RATS, SPRAGUE-DAWLEY; STROMAL CELLS; TRANSFECTION;

RATTUS;

EID: 78751680267     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.1526     Document Type: Article

References (38)

1. Thomas CE, Ehrhardt A, Kay MA. Progress and problems with the use of viral vectors for gene therapy. Nat Rev Genet 2003; 4: 346-358.
2. Lungwitz U, Breunig M, Blunk T, et al. Polyethylenimine-based non-viral gene delivery systems. Eur J Pharm Biopharm 2005; 60: 247-266.
3. Kakudo T, Chaki S, Futaki S, et al. Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system. Biochemistry 2004; 43: 5618-5628.
4. Hyndman L, Lemoine JL, Huang L, et al. HIV-1 Tat protein transduction domain peptide facilitates gene transfer in combination with cationic liposomes. J Control Release 2004; 99: 435-444.
5. Kunath K, Merdan T, Hegener O, et al. Integrin targeting using RGD-PEI conjugates for in vitro gene transfer. J Gene Med 2003; 5: 588-599.
6. Opanasopit P, Rojanarata T, Apirakaramwong A, et al. Nuclear localization signal peptides enhance transfection efficiency of chitosan/DNA complexes. Int J Pharm 2009; 382: 291-295.
7. Yamada Y, Nomura T, Harashima H, et al. Intranuclear DNA release is a determinant of transfection activity for a non-viral vector: biocleavable polyrotaxane as a supramolecularly dissociative condenser for efficient intranuclear DNA release. Biol Pharm Bull 2010; 33: 1218-1222.
8. Jeong JH, Kim SH, Christensen LV, et al. Reducible poly(amido ethylenimine)-based gene delivery system for improved nucleus trafficking of plasmid DNA. Bioconjug Chem 2010; 21: 296-301.
9. Chen TH, Bae Y, Furgeson DY. Intelligent biosynthetic nanobiomaterials (IBNs) for hyperthermic gene delivery. Pharm Res 2008; 25: 683-691.
10. Lee M, Rentz J, Han SO, et al. Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium. Gene Ther 2003; 10: 585-593.
11. Mi Bae Y, Choi H, Lee S, et al. Dexamethasone-conjugated low molecular weight polyethylenimine as a nucleus-targeting lipopolymer gene carrier. Bioconjug Chem 2007; 18: 2029-2036.
12. Neamnark A, Suwantong O, Bahadur RK, et al. Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression. Mol Pharm 2009; 6: 1798-1815.
13. Khalil IA, Kogure K, Akita H, et al. Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery. Pharmacol Rev 2006; 58: 32-45.
14. Brunner S, Furtbauer E, Sauer T, et al. Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation. Mol Ther 2002; 5: 80-86.
15. Brunner S, Sauer T, Carotta S, et al. Cell cycle dependence of gene transfer by lipoplex polyplex and recombinant adenovirus. Gene Ther 2000; 7: 401-407.
16. Mortimer I, Tam P, MacLachlan I, et al. Cationic lipid-mediated transfection of cells in culture requires mitotic activity. Gene Ther 1999; 6: 403-411.
17. Tseng WC, Haselton FR, Giorgio TD. Mitosis enhances transgene expression of plasmid delivered by cationic liposomes. Biochim Biophys Acta 1999; 1445: 53-64.
18. James MB, Giorgio TD. Nuclear-associated plasmid, but not cell-associated plasmid, is correlated with transgene expression in cultured mammalian cells. Mol Ther 2000; 1: 339-346.
19. Clements BA, Hsu CY, Kucharski C, et al. Nonviral delivery of basic fibroblast growth factor gene to bone marrow stromal cells. Clin Orthop Relat Res 2009; 467: 3129-3137.
20. Hsu CY, Uludag H. Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers. BMC Biotechnol 2008; 8: 23.
21. Akita H, Ito R, Kamiya H, et al. Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression. J Gene Med 2007; 9: 197-207.
22. Glover DJ, Leyton DL, Moseley GW, et al. The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell level. J Gene Med 2010; 12: 77-85.
23. Fukata Y, Fukata M. Protein palmitoylation in neuronal development and synaptic plasticity. Nat Rev Neurosci 2010; 11: 161-175.
24. Levental I, Grzybek M, Simons K. Greasing their way: lipid modifications determine protein association with membrane rafts. Biochemistry 2010; 49: 6305-6316.
25. Daum G. Lipids of mitochondria. Biochim Biophys Acta 1985; 822: 1-42.
26. Kamiya H, Fujimura Y, Matsuoka I, et al. Visualization of intracellular trafficking of exogenous DNA delivered by cationic liposomes. Biochem Biophys Res Commun 2002; 298: 591-597.
27. Itaka K, Harada A, Yamasaki Y, et al. In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine. J Gene Med 2004; 6: 76-84.
28. Huang M, Fong CW, Khor E, et al. Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation. J Control Release 2005; 106: 391-406.
29. Cho YW, Kim JD, Park K. Polycation gene delivery systems: escape from endosomes to cytosol. J Pharm Pharmacol 2003; 55: 721-734.
30. Bengali Z, Rea JC, Gibly RF, et al. Efficacy of immobilized polyplexes and lipoplexes for substrate-mediated gene delivery. Biotechnol Bioeng 2009; 102: 1679-1691.
31. Akita H, Ito R, Kamiya H, et al. Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic tipid-mediated transgene expression. J Gene Med 2007; 9: 197-207.
32. Hama S, Akita H, Iida S, et al. Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex. Nucleic Acids Res 2007; 35: 1533-1543.
33. Douglas KL, Piccirillo CA, Tabrizian M. Effects of alginate inclusion on the vector properties of chitosan-based nanoparticles. J Control Release 2006; 115: 354-361.
34. Dastan T, Turan K. In vitro characterization and delivery of chitosan-DNA microparticles into mammalian cells. J Pharm Pharm Sci 2004; 7: 205-214.
35. Rejman J, Bragonzi A, Conese M. Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes. Mol Ther 2005; 12: 468-474.
36. Medina-Kauwe LK. Endocytosis of adenovirus and adenovirus capsid proteins. Adv Drug Deliv Rev 2003; 55: 1485-1496.
37. Rentsendorj A, Agadjanian H, Chen X, et al. The Ad5 fiber mediates nonviral gene transfer in the absence of the whole virus, utilizing a novel cell entry pathway. Gene Ther 2005; 12: 225-237.
38. Hamm-Alvarez SF, Xie J, Wang Y, et al. Modulation of secretory functions in epithelia by adenovirus capsid proteins. J Control Release 2003; 93: 129-140.