Tunable recombinant protein expression with E. Coli in a mixed-feed environment

Applied Microbiology and Biotechnology

Volumn 98, Issue 7, 2014, Pages 2937-2945

  Sagmeister, Patrick ^a   Schimek, Clemens ^a   Meitz, Andrea ^b   Herwig, Christoph ^a   Spadiut, Oliver ^a  

^a VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^b RESEARCH CENTER PHARMACEUTICAL ENGINEERING GMBH   (Austria)

Author keywords

Bioprocess technology; Escherichia coli; Flow cytometry; Mixed feed; Multivariate data analysis; Tunable recombinant protein expression

Indexed keywords

ESCHERICHIA COLI; FLOW CYTOMETRY; MULTIVARIANT ANALYSIS; PRODUCTIVITY; RECOMBINANT PROTEINS;

BIOPROCESSES; EXPRESSION SYSTEM; GREEN FLUORESCENT PROTEIN; MULTIVARIATE DATA ANALYSIS; PROCESS TIME REDUCTION; RECOMBINANT PROTEIN EXPRESSION; RECOMBINANT PROTEIN PRODUCTIONS; SPECIFIC GROWTH RATE;

PROCESS CONTROL;

ARABINOSE; GLUCOSE; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

BACTERIOLOGY; BIOTECHNOLOGY; COST-BENEFIT ANALYSIS; FECAL COLIFORM; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC ANALYSIS; GROWTH RATE; PRODUCTIVITY; PROTEIN; RECOMBINATION;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOMASS PRODUCTION; BIOPROCESS; CARBOHYDRATE METABOLISM; ELECTROPHORESIS; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUOROMETRY; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ARABINOSE; BACTERIOLOGICAL TECHNIQUES; BIOTECHNOLOGY; CARBON; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLUCOSE; GREEN FLUORESCENT PROTEINS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 84898927350     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5445-1     Document Type: Article

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