Advances in Escherichia coli production of therapeutic proteins

Current Opinion in Biotechnology

Volumn 12, Issue 2, 2001, Pages 195-201

  Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH HORMONE; INSULIN; RECOMBINANT PROTEIN;

CELL STRUCTURE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE DOSAGE; GENETIC STABILITY; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SECRETION; PROTEIN SYNTHESIS; REVIEW; RNA TRANSLATION;

BOVINAE; ESCHERICHIA COLI;

EID: 0035313153     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(00)00199-3     Document Type: Review

References (56)

1. Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography
2. Commercial-scale refolding of recombinant methionyl bovine somatotropin
3. Recombinant protein expression in Escherichia coli
4. Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli
5. Secretory production of human leptin in Escherichia coli
6. Construction and characterization of F plasmid-based expression vectors
7. mRNA stability and plasmid copy number effects on gene expression from an inducible promoter system
8. High level expression of eukaryotic polypeptides from bacterial chromosomes
9. Repressor titration: A novel system for selection and stable maintenance of recombinant plasmids
10. BAD promoter
11. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations
12. Library of synthetic 5′ secondary structures to manipulate mRNA stability in Escherichia coli
13. The C-terminal half of RNase E, which organizes the Escherichia coli degradosome, participates in mRNA degradation but not rRNA processing in vivo
14. Functional importance of RNA interactions in selection of translation initiation codons
15. Translational enhancement by an element downstream of the initiation codon in Escherichia coli
16. Secondary structure of the ribosome binding site determines translational efficiency: A quantitative analysis
17. mRNAs can be stabilized by DEAD-box proteins
18. Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces cerevisiae, is an RNA helicase
19. Are DEAD-box proteins becoming respectable helicases?
20. Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DskA, and a truncated R1pA
21. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6Kγ origin plasmids at different copy numbers
22. Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in Escherichia coli dense cultures
23. Pathway engineering for the production of aromatic compounds in Escherichia coli
24. Reduction of aerobic acetate production by Escherichia coli
25. Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction
26. Expression of intracellular hemoglobin improves protein synthesis in oxygen-limited Escherichia coli
27. Expression of Vitreoscilla hemoglobin in Escherichia coli enhances ribosome and tRNA levels: A flow field-flow fractionation study
28. Expression of Alcaligenes eutrophus flavohemoprotein and engineered Vitreoscilla hemoglobin-reductase fusion protein for improved hypoxic growth of Escherichia coli
29. The quiescent-cell expression system for protein synthesis in Escherichia coli
30. Advances in refolding of proteins produced in E. coli
31. Inhibition of aggregation side reactions during in vitro protein folding
32. Renaturation of Escherichia coli-derived recombinant human macrophage colony-stimulating factor
33. Refolding, purification, and characterization of human recombinant PDE4A constructs expressed in Escherichia coli
34. Folding of a synthetic parallel beta-roll protein
35. Oxidative refolding chromatography: Folding of the scorpion toxin Cn5
36. Cold-inducible cloning vectors for low-temperature protein expression in Escherichia coli: Application to the production of a toxic and proteolytically sensitive fusion protein
37. Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli
38. Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm
39. Expression of active human tissue type plasminogen activator in Escherichia coli
40. Overexpression of Escherichia coli oxidoreductases increases recombinant insulin-like growth factor I accumulation
41. The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase Fkp I: Increased functional expression of antibody fragments with and without cis-prolines
42. TolAIII co-overexpression facilitates the recovery of periplasmic recombinant proteins into the growth medium of Escherichia coli
43. Extracellular production of a hybrid β-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors
44. High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium
45. Cell-free protein synthesis systems
46. Cell-free production and stable-isotope labeling of milligram quantities of proteins
47. A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane
48. A continuous cell-free translation system capable of producing polypeptides in high yield
49. Prolonging cell-free protein synthesis with a novel ATP regeneration system
50. Prolonging cell-free protein synthesis by selective reagent additions
51. Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli
52. Efficient coupled transcription/translation from PCR template by a hollow-fiber membrane bioreactor
53. Recent advances in producing and selecting functional proteins by using cell-free translation
54. Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones
55. Co-translational folding of a eukaryotic multidomain protein in a prokaryotic translation system