Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity araE transporter

Microbiology

Volumn 147, Issue 12, 2001, Pages 3241-3247

  Khlebnikov, A ^a   Datsenko, K A ^a   Skaug, T ^a   Wanner, B L ^a   Keasling, J D ^a  

^a University of California   (United States)

Author keywords

Arabinose transport system; FACS analysis; GFP fusions; Red recombination; Regulatable gene expression

Indexed keywords

ARABINOSE;

ARTICLE; CELL POPULATION; CHROMOSOME; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION SYSTEM; GENETIC CODE; HOMOGENATE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 0035216867     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-147-12-3241     Document Type: Article

References (16)

1. Positively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum
2. The complete genome sequence of Escherichia coli K-12
3. R cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant
4. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
5. BAD promoter
6. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r
7. Genetic reconstitution of the high-affinity L-arabinose transport system
8. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters
9. Artificial promoters for metabolic optimization
10. A regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture
11. Production characteristics of interferon-alpha using an L-arabinose promoter system in a high-cell-density culture
12. Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator
13. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations
14. Construction and characterization of regulated L-arabinose-inducible broad host range expression vectors in Xanthomonas
15. Gene expression in bacteria using TnphoA and TnphoA′ elements to make and switch phoA gene, lacZ (op), and lacZ (pr) fusions