Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture

Journal of Bacteriology

Volumn 182, Issue 24, 2000, Pages 7029-7034

  Khlebnikov, A ^a   Risa, O ^a   Skaug, T ^a   Carrier, T A ^a   Keasling, J D ^a  

^a University of California   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABINOSE; CARRIER PROTEIN; PYRANOSIDE; THIOGLYCOSIDE;

ARTICLE; CELL CULTURE; CELL SPECIFICITY; CELL SUBPOPULATION; CONCENTRATION (PARAMETERS); ESCHERICHIA COLI; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENETIC CODE; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE;

ARABINOSE; ARAC TRANSCRIPTION FACTOR; BACTERIAL PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, BACTERIAL; MONOSACCHARIDE TRANSPORT PROTEINS; PLASMIDS; PROMOTER REGIONS (GENETICS); REPRESSOR PROTEINS; TRANSCRIPTION FACTORS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034461764     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.24.7029-7034.2000     Document Type: Article

References (18)

1. Investigating autocatalytic gene expression systems through mechanistic modeling
2. Library of synthetic 5′ secondary structures to manipulate mRNA stability in Escherichia coli
3. BAD promoter
4. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r
5. Genetic reconstitution of the high-affinity L-arabinose transport system
6. Gene expression tools for metabolic engineering of bacteria
7. L-Arabinose transport systems in Escherichia coli K-12
8. Regulation of the L-arabinose transport operons in Escherichia coli
9. Identification of the AraE transport protein of Escherichia coli
10. The cloning, DNA sequence, and overexpression of the gene araE coding for arabinose-proton symport in Escherichia coli K12
11. Distribution of suboptimally induced β-D-galactosidase in Escherichia coli. The enzyme content of individual cells
12. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants
13. Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator
14. Enzyme induction as an all-or-none phenomenon
15. A versatile quick-prep of genomic DNA from Gram-positive bacteria
16. An L-arabinose binding protein and arabinose permeation in Escherichia coli
17. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations