Enhancing the protein production levels in Escherichia coli with a strong promoter

FEBS Journal

Volumn 278, Issue 5, 2011, Pages 729-739

  Tegel, Hanna ^a   Ottosson, Jenny ^a   Hober, Sophia ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Escherichia coli; promoter; protein production; transcription; translation

Indexed keywords

BACTERIAL PROTEIN; BETA GALACTOSIDASE; RNA POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEIN TARGETING; SOLUBILITY; STRAIN DIFFERENCE;

BACTERIAL PROTEINS; ESCHERICHIA COLI; PROMOTER REGIONS, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79951927089     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2010.07991.x     Document Type: Article

References (30)

1. Studier FW, &, Moffatt BA, (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-130.
2. Gronenborn B, (1976) Overproduction of phage lambda repressor under control of the lac promotor of Escherichia coli. Mol Gen Genet 148, 243-250.
3. Wanner BL, Kodaira R, &, Neidhardt FC, (1977) Physiological regulation of a decontrolled lac operon. J Bacteriol 130, 212-222. (Pubitemid 8093451)
4. Brosius J, Erfle M, &, Storella J, (1985) Spacing of the -10 and -35 regions in the tac promoter. Effect on its in vivo activity. J Biol Chem 260, 3539-3541. (Pubitemid 15114354)
5. Golomb M, &, Chamberlin M, (1974) Characterization of T7-specific ribonucleic acid polymerase. IV. Resolution of the major in vitro transcripts by gel electrophoresis. J Biol Chem 249, 2858-2863.
6. Hannig G, &, Makrides SC, (1998) Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol 16, 54-60. (Pubitemid 28055472)
7. Terpe K, (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 72, 211-222. (Pubitemid 44273045)
8. Dubendorff JW, &, Studier FW, (1991) Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J Mol Biol 219, 45-59. (Pubitemid 121003972)
9. Sorensen HP, &, Mortensen KK, (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115, 113-128. (Pubitemid 39656438)
10. Pedelacq JD, Piltch E, Liong EC, Berendzen J, Kim CY, Rho BS, Park MS, Terwilliger TC, &, Waldo GS, (2002) Engineering soluble proteins for structural genomics. Nat Biotechnol 20, 927-932. (Pubitemid 35001131)
11. Alzari PM, Berglund H, Berrow NS, Blagova E, Busso D, Cambillau C, Campanacci V, Christodoulou E, Eiler S, Fogg MJ, et al. (2006) Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE. Acta Crystallogr D Biol Crystallogr 62, 1103-1113. (Pubitemid 44526203)
12. Graslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, et al. (2008) Protein production and purification. Nat Methods 5, 135-146.
13. Graslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, Hammarstrom M, Johansson I, Kotenyova T, Nilsson M, Nordlund P, et al. (2008) The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expr Purif 58, 210-221.
14. Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, &, Gileadi O, (2010) High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol 172, 3-13.
15. Cornvik T, Dahlroth SL, Magnusdottir A, Flodin S, Engvall B, Lieu V, Ekberg M, &, Nordlund P, (2006) An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries. Proteins 65, 266-273. (Pubitemid 44454101)
16. Cornvik T, Dahlroth SL, Magnusdottir A, Herman MD, Knaust R, Ekberg M, &, Nordlund P, (2005) Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli. Nat Methods 2, 507-509.
17. Hammarstrom M, Woestenenk EA, Hellgren N, Hard T, &, Berglund H, (2006) Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein. J Struct Funct Genomics 7, 1-14. (Pubitemid 44273858)
18. Agaton C, Galli J, Hoiden Guthenberg I, Janzon L, Hansson M, Asplund A, Brundell E, Lindberg S, Ruthberg I, Wester K, et al. (2003) Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues. Mol Cell Proteomics 2, 405-414.
19. Stenvall M, Steen J, Uhlen M, Hober S, &, Ottosson J, (2005) High-throughput solubility assay for purified recombinant protein immunogens. Biochim Biophys Acta 1752, 6-10. (Pubitemid 41207244)
20. Hedhammar M, Stenvall M, Lonneborg R, Nord O, Sjolin O, Brismar H, Uhlen M, Ottosson J, &, Hober S, (2005) A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein. J Biotechnol 119, 133-146. (Pubitemid 41219542)
21. Tegel H, Tourle S, Ottosson J, &, Persson A, (2010) Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3). Protein Expr Purif 69, 159-167.
22. Welch M, Govindarajan S, Ness JE, Villalobos A, Gurney A, Minshull J, &, Gustafsson C, (2009) Design parameters to control synthetic gene expression in Escherichia coli. PLoS ONE 4, e7002.
23. Ringquist S, Shinedling S, Barrick D, Green L, Binkley J, Stormo GD, &, Gold L, (1992) Translation initiation in Escherichia coli: sequences within the ribosome-binding site. Mol Microbiol 6, 1219-1229.
24. Mattanovich D, Weik R, Thim S, Kramer W, Bayer K, &, Katinger H, (1996) Optimization of recombinant gene expression in Escherichia coli. Ann NY Acad Sci 782, 182-190. (Pubitemid 26194715)
25. Garcia-Fruitos E, Martinez-Alonso M, Gonzalez-Montalban N, Valli M, Mattanovich D, &, Villaverde A, (2007) Divergent genetic control of protein solubility and conformational quality in Escherichia coli. J Mol Biol 374, 195-205. (Pubitemid 47610779)
26. Ruther U, (1982) pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res 10, 5765-5772.
27. Sambrook J, Fritsch EF, &, Maniatis T, (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.
28. Livak KJ, &, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402-408. (Pubitemid 34164012)
29. Porath J, Carlsson J, Olsson I, &, Belfrage G, (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258, 598-599.
30. Steen J, Uhlen M, Hober S, &, Ottosson J, (2006) High-throughput protein purification using an automated set-up for high-yield affinity chromatography. Protein Expr Purif 46, 173-178.