Efficient feeding profile optimization for recombinant protein production using physiological information

Bioprocess and Biosystems Engineering

Volumn 35, Issue 9, 2012, Pages 1637-1649

  Wechselberger, Patrick ^a   Sagmeister, Patrick ^a   Engelking, Helge ^b   Schmidt, Torsten ^b   Wenger, Jana ^b   Herwig, Christoph ^a  

^a VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^b LONZA AG   (Switzerland)

Author keywords

Alkaline phosphatase; Bioprocess optimization; Design of experiments; Design space development; E. coli; Feed profile feed strategy optimization; Multivariate data analysis; Process analytical technology; Quality by design; RhaBAD promoter

Indexed keywords

ALKALINE PHOSPHATASE; BIOPROCESS OPTIMIZATION; DESIGN SPACES; E. COLI; MULTIVARIATE DATA ANALYSIS; PROCESS ANALYTICAL TECHNOLOGY; QUALITY BY DESIGNS; RHABAD PROMOTER; STRATEGY OPTIMIZATION;

DESIGN OF EXPERIMENTS; DROPS; ESCHERICHIA COLI; FACTOR ANALYSIS; FEEDING; MULTIVARIANT ANALYSIS; OPTIMIZATION; PHOSPHATASES; PHYSIOLOGY; QUALITY CONTROL; RECOMBINANT PROTEINS;

PHYSIOLOGICAL MODELS;

ALKALINE PHOSPHATASE; BACTERIAL PROTEIN; RHAMNOSE;

ARTICLE; BIOMASS; BIOPROCESS; BIOREACTOR; CELL METABOLISM; CLINICAL ASSESSMENT TOOL; ENZYME ACTIVITY; ESCHERICHIA COLI; FEEDING; MULTIVARIATE ANALYSIS; NONHUMAN; OPTICAL DENSITY; PHYSIOLOGY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; VOLUMETRY;

ALKALINE PHOSPHATASE; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; MODELS, BIOLOGICAL; RECOMBINANT PROTEINS; RHAMNOSE;

EID: 84870356622     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-012-0754-9     Document Type: Article

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