Gastro protective, antiapoptotic and anti-inflammatory effect of alpha-lipoic acid on ethanol induced gastric mucosal lesions in rats

American Journal of Biochemistry and Molecular Biology

Volumn 4, Issue 2, 2014, Pages 48-63

  Hussein, S A ^a   El Senosy, Y A ^a   Hassan, Marwa F ^a  

^a BENHA UNIVERSITY   (Egypt)

Author keywords

Antioxidant enzymes; Apoptosis; Ethanol; Pro inflammatory cytokines; lipoic acid

Indexed keywords

ALCOHOL; ASCORBIC ACID; CATALASE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; INTERLEUKIN 6; MALONALDEHYDE; MYELOPEROXIDASE; NITRIC OXIDE; SIALIC ACID; SUPEROXIDE DISMUTASE; THIOCTIC ACID; TUMOR NECROSIS FACTOR ALPHA;

ALCOHOL CONSUMPTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIAPOPTOTIC ACTIVITY; ANTIINFLAMMATORY ACTIVITY; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; BLOOD LEVEL; BLOOD SAMPLING; CONTROLLED STUDY; DNA FRAGMENTATION; DRUG ACTIVITY; ENZYME ACTIVITY; MALE; MALONALDEHYDE BLOOD LEVEL; NITRIC OXIDE BLOOD LEVEL; NONHUMAN; PROTEIN BLOOD LEVEL; RAT; SIALIC ACID BLOOD LEVEL; STOMACH EROSION; STOMACH PROTECTION; TISSUE LEVEL; TREATMENT DURATION;

EID: 84898851163     PISSN: 21504210     EISSN: 21504253     Source Type: Journal    
DOI: 10.3923/ajbmb.2014.48.63     Document Type: Article

References (90)

1. Al Rasheed, N, N.A.A. Baky, N. Al Rasheed, W. Shebly, A.M. Ahmed and L.M. Faddah, 2012. Effect of vitamin E and a-lipoic acid on nano zinc oxide induced renal cytotoxicity in Rats. Afr. J. Pharm. Pharmacol, 6: 2211-2223.
2. Ah, T. and RF. Harty, 2009. Stress-induced ulcer bleeding in critically ill patients. Gastroenterol. Clin. North Am, 38: 245-265.
3. Allavena, P, C. Garlanda, M.G. Borrello, A. Sica and A. Mantovani, 2008. Pathways connecting inflammation and cancer. Curr. Opin. Genet. Dev, 18: 3-10.
4. Amudhan, M.S. and V.H. Begum, 2008. Protective effect of Areca catechu extract on ethanol induced gastric mucosal lesions in rats. Pharmacologyonline, 1: 97-106.
5. Anbar, M. and P. Neta, 1967. The production and use of F-18 labelled organic compounds. Int. J. Appl. Rad. Isot. 18: 495-523.
6. Arafa, H.M.M. and M.M. Sayed-Ahmed, 2003. Protective role of carnitine esters against Alcohol-induced gastric lesions in rats. Pharmacol. Res., 48: 285-290.
7. Arivazhagan, P., T. Thilakavathy, K. Ramanathan, S. Kumaran and C. Panneerselvam, 2002. Effect of DL-a-lipoic acid on the status of lipid peroxidation and protein oxidation in various brain regions of aged rats. J. Nutri. Biochem., 13: 619-624.
8. Bagchi, D, T.R. McGinn, X. Ye, J. Balmoon and M. Bagchi et al, 1999. Mechanism of gastroprotection by bismuth subsalicylate against chemically induced oxidative stress in cultured human gastric mucosal cells. Digestive Dis. Sci., 44: 2419-2428.
9. Banan, A., J.Z. Fields, H. Decker, Y. Zhang and A. Keshavarzian, 2000. Nitric oxide and its metabolites mediate Ethanol-induced microtubule disruption and intestinal barrier dysfunction. J. Pharmacol. Exp. Therapeutics, 294: 997-1008.
10. Bandyopadhyay, U., D. Das and R.K. Banerjee, 1999. Reactive oxygen species: Oxidative damage and pathogenesis. Curr. Sci., 77: 658-666.
11. Beckman, J.S., T.W. Beckman, J. Chen, P.A. Marshall and B.A. Freeman, 1990. Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric oxide and superoxide. Proc. Natl. Acad. Sci. USA., 87: 1620-1624.
12. Beckman, J.S. and W.H. Koppenol, 1996. Nitric oxide, superoxide and peroxynitrite: The good, the bad and ugly. Am. J. Physiol. Cell. Physiol, 271: C1424-C1437.
13. Beyaert, R. and W. Fiers, 1998. Tumor Necrosis Factor and Lymphotoxin. In: Cytokines, Mire-Sluis, A.R. and R. Thorpe (Eds.). Academic Press, San Diego, California, pp: 335-360.
14. Biewenga, G.P, J. Dejong and A. Bast, 1994. Lipoic acid favors thiolsulfinate formation after hypochlorous acid scavenging: A study with lipoic acid derivatives. Arch. Biochem. Biophys, 312: 114-120.
15. Biewenga, G.P, G.R.M.M. Haenen and A. Bast, 1997. The pharmacology of the antioxidant lipoic acid. Gen. Pharmacol, 29: 315-331.
16. Brzozowski, T, S.J. Konturek, S. Kwiecien, R. Pajdo, D. Drozdowicz, Z. Sliwowski and M. Muramatsu, 1998. SU-840, a novel synthetic flavonoid derivative of sophoradin, with potent gastroprotective and ulcer healing activity. J. Physiol. Pharmacol, 49: 83-98.
17. Cadirci, E, H. Suleyman, H. Aksoy, Z. Hahci, U. Ozgen, A. Koc and N. Ozturk, 2007. Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats. Chem. Biol. Interact, 170: 40-48.
18. Chan, D.W.Y. and M.T. Perlstein, 1987. Immunoassay: A Practical Guide. Academic Press, New York, pp: 71.
19. Chatterjee, M, R. Saluja, S. Kanneganti, S. Chinta and M. Dikshit, 2007. Biochemical and molecular evaluation of neutrophil NOS in spontaneously hypertensive rats. Cell. Mol. Biol, 53: 84-93.
20. +-ATPase, oxidative damage and apoptosis. Inflammopharmacology, 12: 153-176.
21. Choi, J.I, H.R.B. Raghavendran, N.Y. Sung, J.H. Kim and B.S. Chun et al, 2010. Effect of fucoidan on aspirin-induced stomach ulceration in rats. Chemico-Biol. Int., 183: 249-254.
22. Cross, C, B. Halliwell and A. Allen, 1984. Antioxidant protection: Afunction of tracheobronchial and gastrointestinal mucus. Lancet, 323: 1328-1330.
23. Cui, B., S. Liu, X. Lin, J. Wang, S. Li, Q. Wang and S. Li, 2011. Effects of Lycium barbarum aqueous and ethanol extracts on High-fat-diet induced oxidative stress in rat liver tissue. Molecules, 16:9116-9128.
24. David, M. and J.S. Richard, 1983. Glutathione Reductase. In: Methods of Enzymatic Analysis, Bergmeyer, H.U., J. Bergmeyer and M. Grassl (Eds.). Verlag Chemie, Weinheim, pp: 258-265.
25. DeMarco, V.G., P.O. Scumpia, J.P. Bosanquet and J.W. Skimming, 2004. A-Lipoic acid inhibits Endotoxin-stimulated expression of iNOS and nitric oxide independent of the heat shock response in RAW 264.7 cells. Free Radical Res., 38: 675-682.
26. Dey, A. and A.I. Cederbaum, 2006. Alcohol and oxidative liver injury. Hepatology, 43: S63-S74.
27. Eguchi, H., Y. Ikeda, T. Ookawara, S. Koyota and N. Fujiwara et al., 2005. Modification of oligosaccharides by reactive oxygen species decreases sialyl lewis x-mediated cell adhesion. Glycobiology, 15: 1094-1101.
28. Enomoto, N, K. Ikejima, T. Kitamura, H. Oide, Y. Takei, N. Sato and R.G. Thurman, 2000. Alcohol enhances lipopolysacch-ide induced increases in nitric oxide production by Kupfer cells via mechanism dependent on endotoxin. Alcohol. Clin. Exp. Res., 24: 55S-58S.
29. Esrefoglu, M., M. Gul, B. Ates and I. Yilmaz, 2006. Ultrastructural clues for the protective effect of ascorbic acid and N-acetylcysteine against oxidative damage on Caerulein-induced pancreatitis. Pancreatology, 6: 477-485.
30. Freeman, B.A. and J.D. Crapo, 1982. Biology of disease: Free radicals and tissue injury. Lab. Invest., 47: 412-426.
31. Frei, B., L. England and B.N. Ames, 1989. Ascorbate is an outstanding antioxidant in human blood plasma. Proc. Natl. Acad. Sci. USA, 86: 6377-6381.
32. Fukumura, D., I. Kurose, S. Miura, M. Tsuchiya and H. Ishii, 1995. Oxidative stress in gastric mucosal injury: Role of platelet-activating Factor-activated granulocytes. J. Gastroenterol., 30: 565-571.
33. Goel, R.K., R.N. Maiti and K. Mukhopadhyaya, 1994. Effect of Tamrabhasma, an Indian indigenous preparation of copper, on rat gastric mucosal resistance. Indian J. Exp. Biol., 32: 559-561.
34. Gross, R.T., R. Bracci, N. Rudolph, E. Schroeder and J.A. Kochen, 1967. Hydrogen peroxide toxicity and detoxification in the erythrocytes of newborn infants. Blood, 29: 481-493.
35. Ham, M. and J.D. Kaunitz, 2007. Gastroduodenal defense. Curr. Opin. Gastroenterol., 23: 607-616.
36. Han, D., G. Handelman, L. Marcocci, C.K. Sen and S. Roy et al., 1997. Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization. Biofactors, 6: 321-338.
37. Hodgson, E.K. and I. Fridovich, 1975. Interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide. Inactivation of the enzyme. Biochemistry, 14: 5294-5299.
38. Jaeschke, H., 1995. Mechanisms of oxidant stress-induced acute tissue injury. Proc. Soc. Exp. Biol. Med, 209: 104-111.
39. Johansen, J.S, A.K. Harris, D.J. Rychly and A. Ergul, 2005. Oxidative stress and the use of antioxidants in diabetes: Linking basic science to clinical practice. Cardiovascular Diabetol, Vol. 4. 10.1186/1475-2840-4-5
40. Jones, W, X. Li, Z.C. Qu, L. Pernott, R.R. Whitesell and J.M. May, 2002. Uptake, recycling and antioxidant actions of a-lipoic acid in endothelial cells. Free Radical Biol. Med, 33: 83-93.
41. Kakkar, P, B. Das and P.N. Viswanathan, 1984. A modified spectrophotometric assay of superoxide dismutase. Indian J. Biochem. Biophys, 21: 130-132.
42. Kalia, N., K.D. Bardhan, M.W. Reed, S. Jacob and N.J. Brown, 2000. Mast cell stabilization prevents ethanol-induced rat gastric mucosal injury: Mechanisms of protection. J. Gastroenterol. Hepatol., 15: 133-141.
43. Karakoyun, B., M. Yuksel, F. Ercan, C. Erzik and B.C. Yegen, 2009. Alpha-lipoic acid improves acetic Acid-induced gastric ulcer healing in rats. Inflammation, 32: 37-46.
44. Karmeli, F., E. Okon and D. Rachmilewitz, 1996. Sulphydryl blocker induced gastric damage is ameliorated by scavenging of free radicals. Gut, 38: 826-831.
45. Khazaei, M. and H. Salehi, 2006. Protective effect of falcaria vulgaris extract on ethanol induced gastric ulcer in rat. Iran. J. Pharmacol. Ther., 5: 43-46.
46. Knoll, M.R., C.B. Kolbel, S. Teyssen and M.V. Singer, 1998. Action of pure ethanol and some alcoholic beverages on the gastric mucosa in healthy humans: A descriptive endoscopic study. Endoscopy, 30: 293-301.
47. Kohda, K., K. Tanaka, Y. Aiba, M. Yasuda, T. Miwa and Y. Koga, 1999. Role of apoptosis induced by Helicobacter pylori infection in the development of duodenal ulcer. Gut, 44: 456-462.
48. Kojo, S., 2004. Vitamin C: Basic metabolism and its function as an index of oxidative stress. Curr. Med. Chem, 11: 1041-1064.
49. Koppenol, W.H., 1981. Superoxide dismutase and oxygen toxicity. Clin. Respir. Physiol., 17: 85-89.
50. Kryger, G., M. Harel, K. Giles, L. Toker and B. Velan et al., 2000. Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculm-II. Acta Cryst, 56: 1385-1394.
51. Kucharz, E.J., 1992. The Collagens: Biochemistry and Pathophysiology. Springer-Verlag, Berlin, pp: 31-35.
52. Kvietys, P.R., B. Twohig, J. Danzell and R.D. Specian, 1990. Ethanol-induced injury to the rat gastric mucosa. Role of neutrophils and xanthine Oxidase-derived radicals. Gastroenterology, 98: 909-920.
53. Liu, Y., X. Tian, L. Gou, X. Fu, S. Li, N. Lan and X. Yin, 2012. Protective effect of L-citrulline against Ethanol-induced gastric ulcer in rats. Environ. Toxicol. Pharmacol., 34: 280-287.
54. Lu, C. and Y. Liu, 2002. Interactions of lipoic acid radical cations with vitamins C and E analogue and hydroxycinnamic acid derivatives. Arch. Biochem. Biophys., 406: 78-84.
55. Luck, H., 1974. Estimation of Catalase. In: Methods in Enzymatic Analysis, Bergmeyer, H.V. (Ed.). Academic Press, New York, pp: 885.
56. Malinska, D. and K. Winiarska, 2005. Lipoic acid: Characteristics and therapeutic applications. Postepy Hig. Med. Dosw, 59: 535-543.
57. Masuda, E., S. Kawano, K. Nagano, S. Tsuji and Y. Takei et al., 1995. Endogenous nitric oxide modulates ethanol-induced gastric mucosal injury in rats. Gastroenterology, 108: 58-64.
58. Megala, J. and A. Geetha, 2010. Gastro protective and antioxidant effects of hydro alcoholic fruit extract of pithecellobium dulce on ethanol induced gastric ulcer in rats. Pharmacologyonline, 2: 353-372.
59. Mesbah, L., B. Soraya, S. Narimane and P.F. Jean, 2004. Protective effect of flavonides against the toxicity of vinblastine cyclophosphamide and paracetamol by inhibition of lipid-peroxydation and increase of liver glutathione. Haematology, 7: 59-67.
60. Moron, M.S., J.W. Depierre and B. Mannervik, 1979. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica Biophys. Acta-General Subjects, 582: 67-78.
61. Natale, G., G. Lazzeri, C. Blandizzi, G. Gherardi, P. Lenzi, A. Pellegrini and M. del Tacca, 2001. Seriate histomorphometry of whole rat stomach: An accurate and reliable method for quantitative analysis of mucosal damage. Toxicol. Applied Pharmacol., 174: 17-26.
62. Nordberg, J. and E.S. Arner, 2001. Reactive oxygen species, antioxidants and the mammalian thioredoxin system. Free Radic. Biol. Med., 31: 1287-1312.
63. Ogasawara, Y., T. Namai, F. Yoshino, M.C.I. Lee and K. Ishii, 2007. Sialic acid is an essential moiety of mucin as a hydroxyl radical scavenger. FEBS Lett., 581: 2473-2477.
64. Oh, S.I., C.I. Kim, H.J. Chun and S.C. Park, 1998. Chronic ethanol consumption affects glutathione status in rat liver. J. Nutr., 128: 758-763.
65. Ohkura, Y., T. Furihata, H. Kawamata, M. Tabuchi and K. Kubota et al., 2003. Evaluation of cell proliferation and apoptosis in Helicobacter pylori gastritis using an image analysis processor. Gastric Cancer, 6: 49-54.
66. Okado-Matsumoto, A. and I. Fridovich, 2001. Subcellular distribution of superoxide dismutases (SOD) in rat liver: Cu,Zn-SOD in mitochondria. J. Biol. Chem, 276: 38388-38393.
67. Packer, L., K. Kraemer and G. Rimbach, 2001. Molecular aspects of lipoic acid in the prevention of diabetes complications. Nutrition, 17: 888-895.
68. Padayatty, S.J., R. Daruwala and Y.Y. Wang, 2002. Vitamin C: From Molecular Actions to Optimum Intake. In: Handbook of Antioxidants (Oxidative Stress and Disease), Cadenas, E. and L. Packer (Eds.). CRC, Marcel Dekker, Inc., New York.
69. Panda, V., H. Ashar and S. Srinath, 2012. Antioxidant and hepatoprotective effect of Garcinia indica fruit rind in ethanolinduced hepatic damage in rodents. Interdisciplinary Toxicol., 5: 207-213.
70. Pihan, G., C. Regillo and S. Szabo, 1987. Free radicals and lipid peroxidation in ethanol or aspirin-induced gastric mucosal injury. Digestive Dis. Sci., 32: 1395-1401.
71. Prakash, M., G. Gunasekaran and K. Elumalai, 2008. Effect of earthworm powder on antioxidant enzymes in alcohol induced hepatotoxic rats. Eur. Rev. Med. Pharmacol. Sci., 12: 237-243.
72. Rukkumani, R., K. Aruna, P.S. Varma, K.N. Rajasekaran and V.P. Menon, 2004. Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress. J. Pharm. Pharmacol. Sci, 7: 274-283.
73. SPSS, 2009. Analytical Software for Windows SPSS Version 13.0. SPSS, Chicago, IL, USA.
74. Sehirli, O, E. Tathdede, M. Yuksel, C. Erzik, S. Cetmel, B.C. Yegen and G Sener, 2008. Antioxidant effect of alpha-lipoic acid against ethanol-induced gastric mucosal erosion in rats. Pharmacology, 81: 173-180.
75. Selvakumar, E, C. Prahalathan, Y. Mythili and P. Varalakshmi, 2005. Beneficial effects of Dl-a-lipoic acid on cyclophosphamide-induced oxidative stress in mitochondrial fractions of rat testis. Chemico-Biol. Interactions, 152: 59-66.
76. Shay, P.K, R.F. Moreau, E.J. Smith and T.M. Hagen, 2008. Is a-lipoic acid a scavenger of reactive oxygen species in vivo? Evidence for its initiation of stress signaling pathways that promote endogenous antioxidant capacity. IUBMB Life, 60: 362-367.
77. Shi, B, U. de Girolami, J. He, S. Wang, A. Lorenzo, J. Busciglio and D. Gabuzda, 1996. Apoptosis induced by HIV-1 infection of the central nervous system. J. Clin. Invest, 98: 1979-1990.
78. Smith, G.S, D.W. Mercer, J.M. Cross, J.C. Barreto and T.A. Miller, 1996. Gastric injury induced by ethanol and Ischemia-reperfusion in the rat. Digestive Dis. Sci, 41: 1157-1164.
79. Somani, S.M, K. Husain, C. Whitworth, G.L. Trammell, M. Malafa and L.P. Rybak, 2000. Dose-dependent protection by lipoic acid against cisplatin-induced nephrotoxicity in rats: antioxidant defense system. Pharmacol. Toxicol., 86: 234-241.
80. Sumangala, P.R., M.K. Dilip, N.B. Shivanand and S.S. Sathiamoorthy, 1998. Prostaglandin mediated acid secretion inhibitory effect as a possible mechanism for the anticulcer effect of angiotension converting enzyme inhibitor (captopril) in pylorus ligated rats. Indian J. Pharmacol., 30: 385-389.
81. Szabo, S., 1987. Mechanisms of mucosal injury in the stomach and duodenum: Time-sequence analysis of morphologic, functional, biochemical and histochemical studies. Scandinavian J. Gastroenterol., 22: 21-28.
82. Takeuchi, K., S. Ueki and S. Okabe, 1986. Importance of gastric motility in the pathogenesis of indomethacin-induced gastric lesions in rats. Dig. Dis. Sci., 31: 1114-1121.
83. Teixeira, H.D., R.I. Schumacher and R. Meneghini, 1998. Lower intracellular hydrogen peroxide levels in cells overexpressing CuZn-superoxide dismutase. Proc. Natl. Acad. Sci., 95: 7872-7875.
84. Umamaheswari, M., K. Asokkumar, R. Rathidevi, A.T. Sivashanmugam, V. Subhadradevi and T.K. Ravi, 2007. Antiulcer and in vitro antioxidant activities of Jasminum grandiflorum L. J. Ethnopharmacol., 110: 464-470.
85. Vazquez-Ramirez, R., M. Olguin-Martinez, C. Kubli-Garfias and R. Hernandez-Munoz, 2006. Reversing gastric mucosal alterations during Ethanol-induced chronic gastritis in rats by oral administration of Opuntia Ficus-indica mucilage. World J. Gastroenterol., 12: 4318-4324.
86. Vincent, A.M., L.L. McLean, C. Backus and E.L. Feldman, 2005. Short-term hyperglycemia produces oxidative damage and apoptosis in neurons. FASEB J., 19: 638-640.
87. Vodovotz, Y., 1996. Modified microassay for serum nitrite and nitrate. Biotechniques, 20: 390-392, 394.
88. Younan, F., J. Pearson, A. Allen and C. Venables, 1982. Changes in the structure of the mucus gel on the mucosal surface of the stomach in association with peptic ulcer disease. Gastroenterology, 82:827-831.
89. Zima, T., L. Fialova, O. Mestek, M. Janebova and J. Crkovska et al., 2001. Oxidative stress, metabolism of ethanol and alcohol-related diseases. J. Biomed. Sci., 8: 59-70.
90. Zimmerman, B.J. and D.N. Granger, 1994. Oxygen free radicals and the gastrointestinal tract: role in Ischemia-reperfusion injury: The role of free radicals. Hepato-Gastroenterol., 41: 337-342.