Mechanism of antiulcer effect of Neem (Azadirachta indica) leaf extract: Effect on H+-K+-ATPase, oxidative damage and apoptosis

Inflammopharmacology

Volumn 12, Issue 2, 2004, Pages 153-176

  Chattopadhyay, Ishita ^a   Nandi, Bithi ^a   Chatterjee, Ratna ^a   Biswas, Kaushik ^a   Bandyopadhyay, Uday ^a   Banerjee, Ranajit K ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

Author keywords

Apoptosis; Gastric ulcer; H+ K + ATPase; Neem (Azadirachta indica) leaf extract; Oxidative damage

Indexed keywords

ALCOHOL; ANTIULCER AGENT; AZADIRACHTA INDICA EXTRACT; HYDROGEN POTASSIUM ADENOSINE TRIPHOSPHATASE; HYDROXYL RADICAL; IMIDAZOLE DERIVATIVE; INDOMETACIN; MERCAPTOMETHYLIMIDAZOLE; OMEPRAZOLE; RANITIDINE; UNCLASSIFIED DRUG;

ACID SECRETION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; AQUEOUS SOLUTION; ARTICLE; AZADIRACHTA INDICA; COLD STRESS; CONTROLLED STUDY; DISEASE MODEL; DNA DAMAGE; DOSE RESPONSE; DRUG EFFECT; DRUG EFFICACY; ENZYME INHIBITION; IMMOBILIZATION STRESS; LIPID PEROXIDATION; MALE; NONHUMAN; OXIDATION; PLANT LEAF; PRIORITY JOURNAL; PYLORUS LIGATION; RAT; RAT STRAIN; STOMACH ACID; STOMACH ULCER;

ANIMALS; ANTI-ULCER AGENTS; APOPTOSIS; AZADIRACHTA; DOSE-RESPONSE RELATIONSHIP, DRUG; H(+)-K(+)-EXCHANGING ATPASE; MALE; OXIDATIVE STRESS; PLANT EXTRACTS; PLANT LEAVES; RATS; RATS, SPRAGUE-DAWLEY; STOMACH ULCER;

EID: 3242719668     PISSN: 09254692     EISSN: None     Source Type: Journal    
DOI: 10.1163/1568560041352257     Document Type: Article

References (66)

1. Ausubel, F. M., Brent, R., Kingston, R. E., et al. (1998). In: Current Protocols in Molecular Biology, Vol. I, pp. 2.1.1-2.1.2. Wiley, New York, NY.
2. Babbs, C. F. and Steiner, N. G. (1990). Detection and quantitation of hydroxyl radical using dimethylsulfoxide as molecular probe, Methods Enzymol. 186, 137-147.
3. Badam, L., Joshi, S. P. and Bedekar, S. S. (1999). In vitro antiviral activity of Neem (Azadirachta indica, Juss) leaf extract against group B coxsackie viruses, Commun. Dis. 31, 79-90.
4. Balasenthi, L. S., Arivazhagan, S., Ramachandram, C. R., et al. (1999). Chemopreventive potential of Neem (Azadirachta indica) on 7, 12-dimethylbenzanthracene (DMBA) induced hamster buccal pouch carcinogenesis, J. Ethnopharmacol. 67, 189-195.
5. Bandyopadhyay, D., Biswas, K., Bandyopadhyay, U., et al. (2000). Melatonin protects against stress-induced gastric lesions by scavenging the hydroxyl radical, J. Pineal Res. 29, 143-151.
6. Bandyopadhyay, U., Bhattacharyya, D. K., Chatterjee, R., et al. (1992). Localization of gastric peroxidase and its inhibition by mercaptomethylimidazole, an inducer of gastric acid secretion, Biochem. J. 284, 305-312.
7. +-ATPase inhibition and scavenging of hydroxyl radical, Life Sci. 71, 2845-2865.
8. Bandyopadhyay, U., Chatterjee, R., Chakraborty, T. K., et al. (1997). Activation of parietal cell by mercaptomethylimidazole - a novel inducer of gastric acid secretion, Biochem. Pharmacol. 54, 241-248.
9. Banerjee, R. K., Das, P. K. and Bhattacharjee, M. (1990). Gastric peroxidase and its role in cellular control of gastric acid secretion, in: Biological Oxidation Systems, Vol. 1, Channa Reddy, C., Hamilton, G. A. and Madyastha, K. M. (Eds), pp. 505-513. Academic Press, San Diego, CA.
10. Berglindh, T. (1977). Effect of common inhibitors of gastric acid secretion on secretagogue-induced respiration and aminopyrine accumulation in isolated gastric glands, Biochim. Biophys. Acta 464, 217-233.
11. Bhattacharjee, M., Bhattacharjee S., Gupta, A., et al. (2002). Critical role of an endogenous gastric peroxidase in controlling oxidative damage in H. pylori-mediated and nonmediated gastric ulcer, Free Radic. Biol. Med. 32, 731-743.
12. Bhattacharjee, M., Bose, A. K. and Banerjee, R. K. (1989). Histamine H2-receptor mediated stimulation of gastric acid secretion by mercaptomethylimidazole, Biochem. Pharmacol. 38, 907-914.
13. Biswas, K., Bandyopadhyay, U., Chattopadhyay, I., et al. (2003). A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical, J. Biol. Chem. 278, 10993-11001.
14. Biswas, K., Chattopadhyay, I., Banerjee, R. K., et al. (2002). Biological activities and medicinal properties of Neem (Azadirachta indica), Curr. Sci. 82, 1336-1345.
15. Buege, J. A. and Aust, S. D. (1978). Microsomal lipid peroxidation, Methods Enzymol. 52, 302-310.
16. Burkitt, M. J. and Milne, L. (1996). Hydroxyl radical formation from Cu(II)-trolox mixtures: insights into the pro-oxidant properties of alpha-tocopherol, FEBS Lett. 379, 51-54.
17. Chakrabortty, T. Uerotta, L. and Poddar, G. (1989). Evaluation of Azadirachta indica leaf extract for hypoglycemic activity in rats, Phytother. Res. 3, 30-32.
18. Cho, C. H., Koo, M. W., Garg, G. P., et al. (1992). Stress-induced gastric ulceration: its aetiology and clinical implications, Scand. J. Gastroenterol. 27, 257-262.
19. Chopra, R. N., Chopra, I. C., Handa, K. L., et al. (1958). Indigenous Drugs of India. U.N. Dhar&Sons, Calcutta.
20. Chopra, R. N., Nayar S. L. and Chopra, I. C. (1956). In: Glossary of Indian Medicinal Plants, pp. 31-32. Council of Scientific & Industrial Research, New Delhi.
21. Das, D., Bandyopadhyay, D. and Banerjee, R. K. (1998). Oxidative inactivation of gastric peroxidase by site-specific generation of hydroxyl radical and its role in stress-induced gastric ulceration, Free Radic. Biol. Med. 24, 460-469.
22. Das, D., Bandyopadhyay, D., Bhattacharjee, M. and Banerjee, R. K. (1997). Hydroxyl radical is the major causative factor in stress-induced gastric ulceration, Free Radic. Biol. Med. 23, 8-18.
23. Das, D. and Banerjee, R. K. (1993). Effect of stress on the antioxidant enzymes and gastric ulceration, Mol. Cell. Biochem. 125, 115-125.
24. Devakumar, C. and Sukhdev (1996). Chemistry, in: Neem, 2nd edn, Randhawa, N. S. and Parmar, B. S. (Eds), pp. 77-110. Society of Pesticide Science, New Delhi.
25. +-ATPase, Nature 290, 159-161.
26. Fuji, Y., Matsure, T., Kai, M., et al. (2000). Mitochondrial cytochrome c release and caspase 3 like protease activation during indomethacin-induced apoptosis in rat gastric mucosal cells, Proc. Soc. Exp. Biol. Med. 224, 102-108.
27. Garg, G. P., Nigam, S. K. and Ogle, C. W. (1993). The anti gastric ulcer effects of the leaves of the Neem tree, Planta Med. 59, 215-217.
28. Garg, H. S. and Bhakuni, D. S. (1984). An isoprenylated flavanone from leaves of Azadirachta indica, Phytochemistry 23, 2115-2117.
29. Gogate, S. S. (1996). Cytotoxicity of Neem leaf extract (Azadirachta indica juss): an antitumor activity, Natl. Med. J. Ind. 9, 297.
30. Goodwin, C. S., Armstrong, J. A. and Marshall, B. (1986). Campylobacter pyloridis, gastritis and peptic ulceration, Clin. Pathol. 39, 353-365.
31. Guslandi, M. A. (1999). A radical view of Helicobacter pylori, Am. J. Gastroenterol. 94, 2797-2798.
32. 2- receptor antagonists, in: Ulcer Diseases: Investigation and Basis for Therapy, Swabb, E. A. and Szabo, S. (Eds), pp. 189-215. Marcel Dekker, New York, NY.
33. Ivy, K. M. (1988). Mechanisms of nonsteroidal antiinflammatory drug-induced gastric damage. Actions of therapeutic agents, Am. J. Med. 84, 41-48.
34. Iwu, M. M., Obidoa, O. and Anazodo, M. (1986). Biochemical mechanism of the antimalarial activity of Azadirachta indica leaf extract, Pharmacol. Res. Commun. 18, 81-91.
35. Jones, M. K., Wang, H. and Peskar, B. M. (1999). Inhibition of angiogenesis by nonsteroidal antiinflammatory drugs: Insight into mechanisms and implications for cancer growth and ulcer healing, Nature Med. 5, 1418-1423.
36. Ketkar, A. Y. and Ketkar, C. M. (1995). Various uses of Neem product: Medicinal uses including pharmacology in Asia, in: The Neem Tree, Schmutterer, H. (Ed.), pp. 518-525. VCH, Weinheim.
37. Kirtikar, K. R. and Basu, B. D. (1935). Azadirachta indica, Vol. I, Indian Medicinal Plants, 2nd edn. Lalitha Mohan Basu, Allahabad.
38. Konturek, P. C., Bielansk, W., Konturek, S. J., et al. (1999). Helicobacter pylori associated gastric pathology, J. Physiol. Pharmacol. 50, 695-710.
39. Konturek, P. C., Brzozowski, T., Konturek, S. J., et al. (1999). Apoptosis in gastric mucosa with stress-induced gastric ulcers, J. Physiol. Pharmacol. 50, 211-225.
40. Kusuhara, H., Kimatsu, H., Sumichika, H., et al. (1999). Reactive oxygen species are involved in the apoptosis induced by nonsteroidal antiinflammatory drugs in cultured gastric cells, Eur. J. Physiol. 383, 331-337.
41. Langman, M. T. S., Brooks, P., Hawkey, C. J., et al. (1991). Nonsteroidal antiinflammatory drug associated ulcer epidemiology, causation and treatment, J. Gastroenterol. Hepatol. 6, 442-449.
42. Lowry, O. H., Rosebrough, N. J., Farr, A. L., et al. (1951). Protein measurement with the folin phenol reagent, J. Biol. Chem. 193, 265-275.
43. Miller, T. (1983). Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms, Am. J. Physiol. 235, G601-G623.
44. Miller, T. A. (1987). Mechanism of stress-related mucosal damage, Am. J. Med. 83, 8-14.
45. Miura, T., Muraoka, S. and Fujimoto, Y. (2002). Lipid peroxidation induced by indomethacin with horseradish peroxidase and hydrogen peroxide: involvement of indomethacin radical, Biochem. Pharmacol. 63, 2069-2074.
46. Peskar, B. M., Lange, K., Hoppe, U. and Perkar, B. A. (1986). Ethanol stimulates formation of leukotriene C4 in rat gastric mucosa, Prostaglandins 31, 283-293.
47. Phull, P. S., Green, C. J. and Jacyna, M. R. (1995). A radical view of the stomach: the role of oxygen derived free radicals and antioxidants in gastroduodenal disease, Am. J. Gastroenterol. Hepatol. 7, 265-274.
48. Piham, G., Regillo, C. and Szabo, S. (1987). Free radicals and lipid peroxidation in ethanol and aspirin-induced gastric mucosal injury, Dig. Dis. Sci. 32, 1395-1401.
49. Pillai, N. R. and Shantakumari, G. (1984). Effect of nimbidin on acute and chronic gastroduodenal ulcer models in experimental animals, Planta Med. 50, 143-146.
50. Piotrowski, J., Piotrowski, E., Skrodzka, D., et al. (1997). Gastric mucosal apoptosis induced by ethanol: effect of antiulcer agents, Biochem. Mol. Biol. Int. 42, 247-254.
51. Ray, A., Banerjee, B. D. and Sen, P. (1996). Modulation of humoral and cell mediated immune responses by Azadirachta indica (Neem) in mice, Ind. J. Exp. Biol. 34, 698-701.
52. Rosenfeld, C. G. (1986). Prostaglandin E2 inhibition of secretagogue-stimulated [14-C]-aminopyrine accumulation in rat parietal cell. A model for its mechanism of action, J. Pharmacol. Exp. Ther. 237, 513-518.
53. Sachs, G., Maton, P. N. and Wallmark, B. (1991). Pharmacology of the parietal cell, in: Pharmacology of Peptic Ulcer Disease, Collin, M. J. and Benjamin, S. B. (Eds), pp. 1-54. Springer, New York, NY.
54. Sachs, G. (1997). Proton pump inhibitors and acid related diseases, Pharmacotherapy 17, 22-37.
55. Schmutterer, H. (1995). The Neem Tree. VCH, Weinheim.
56. Shay, H., Sun, D. C. and Gruenstein, A. (1954). A quantitative method for measuring spontaneous gastric secretion in the rat, Gastroenterology 26, 906-913.
57. Slomiany, B. L., Piotrowski, J. and Slomiany, A. (1997). Induction of tumor necrosis factor-α and apoptosis in gastric mucosal injury by indomethacin: effect of omeprazole and ebrotidine, Scand. J. Gastroenterol. 32, 638-642.
58. +)-ATPase from gastric membrane, Biochim. Biophys. Acta 732, 579-585.
59. Szabo, A., Trier, J. S. and Frankel, P. W. (1981). Sulfhydryl compounds may mediate gastric cytoprotection, Science 214, 200-202.
60. Szabo, S. and Tarnawski, A. S. (2000). Apoptosis in the gastric mucosa: molecular mechanisms, basic and clinical implications, J. Physiol. Pharmacol. 51, 3-15.
61. Szabo, S. (1987). Mechanism of mucosal injury in the stomach and duodenum: timesequence analysis of morphologic, functional, biochemical and histochemical studies, Scand. J. Gastroenterol. 127, 21-28.
62. Taussky, H. H. and Shorr, E. J. (1953). A microcalorimetric method for the determination of inorganic phosphate, J. Biol. Chem. 202, 675-685.
63. +-ATPase activity, J. Biol. Chem. 260, 13681-13684.
64. Watanabe, S., Takagi, A., Koga, A., et al. (2000). Helicobacter pylori induces apoptosis in gastric epithelial cells through inducible nitric oxide, Gastroenterol. Hepatol. 15, 168-174.
65. Yamashita, N., Murata, M., Inoue, S., et al. (1998). Alpha-tocopherol induces oxidative damage to DNA in the presence of copper (II) ions, Chem. Res. Toxicol. 11, 855-862.
66. Yoshikawa, T., Naito, Y., Kishi, A., et al. (1993). Role of active oxygen, lipid peroxidation and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats, Gut 34, 732-737.