Generation of a vector suite for protein solubility screening

Frontiers in Microbiology

Volumn 5, Issue FEB, 2014, Pages

  Correa, Agustín ^a   Ortega, Claudia ^a   Obal, Gonzalo ^a   Alzari, Pedro ^b   Vincentelli, Renaud ^c   Oppezzo, Pablo ^a  

^a INSTITUT PASTEUR DE MONTEVIDEO   (Uruguay)

^b INSTITUT PASTEUR   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Cloning; Expression; High throughput; Recombinant proteins; Solubility; Vector

Indexed keywords

GLUCAN SYNTHASE; GREEN FLUORESCENT PROTEIN; MALTOSE BINDING PROTEIN; POLYHISTIDINE TAG; PROTEIN DISULFIDE ISOMERASE; RECOMBINANT PROTEIN; SUMO 2 PROTEIN; THIOREDOXIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CLONING VECTOR; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENHANCER REGION; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE INSERTION; HIGH THROUGHPUT SCREENING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SOLUBILITY; SOLUBILITY;

EID: 84897630667     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2014.00067     Document Type: Article

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