High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Nozach, Hervé ^a   Fruchart Gaillard, Carole ^a   Fenaille, François ^a   Beau, Fabrice ^a   Ramos, Oscar Henrique Pereira ^a   Douzi, Badreddine ^b   Saez, Natalie J ^b   Moutiez, Mireille ^a   Servent, Denis ^a   Gondry, Muriel ^a   Thaï, Robert ^a   Cuniasse, Philippe ^a   Vincentelli, Renaud ^b   Dive, Vincent ^a  

^a SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Cytoplasm; Disulfide bonds; Disulfide rich; Escherichia coli; Fusion protein; High throughput screening; Mass; Matrix assisted laser desorption ionization; Proteins; Recombinant expression; Spectrometry

Indexed keywords

DISULFIDE; DSBC PROTEIN; PROTEIN DISULFIDE ISOMERASE; UNCLASSIFIED DRUG;

ARTICLE; ESCHERICHIA COLI; EX VIVO STUDY; HIGH THROUGHPUT SCREENING; MASS SPECTROMETRY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

CYTOPLASM; DISULFIDES; ESCHERICHIA COLI; OXIDATION-REDUCTION; PROTEIN DISULFIDE-ISOMERASES; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84876734643     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-37     Document Type: Article

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