High-throughput expression screening and purification of recombinant proteins in E. Coli

Methods in Molecular Biology

Volumn 1091, Issue , 2014, Pages 33-53

  Saez, Natalie J ^a   Vincentelli, Renaud ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Auto induction; Bacteria; E. coli; Expression; High throughput; Immobilized metal affinity chromatography (IMAC); Purification; Recombinant; TEV cleavage

Indexed keywords

BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TEMPERATURE; BACTERIAL STRAIN; CULTURE MEDIUM; CULTURE VESSEL; PROTEIN SYNTHESIS; QUALITY CONTROL; REPRODUCIBILITY; SCALE UP;

ESCHERICHIA COLI; GENE EXPRESSION; HIGH-THROUGHPUT SCREENING ASSAYS; QUALITY CONTROL; RECOMBINANT PROTEINS; TRANSFORMATION, BACTERIAL;

EID: 84934435566     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-691-7_3     Document Type: Article

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