Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases

Journal of Structural Biology

Volumn 175, Issue 2, 2011, Pages 113-119

  Luna Vargas, Mark P A ^a   Christodoulou, Evangelos ^a,b   Alfieri, Andrea ^a,c   van Dijk, Willem J ^a   Stadnik, Magda ^a   Hibbert, Richard G ^a   Sahtoe, Danny D ^a   Clerici, Marcello ^a   Marco, Valeria De ^a,b   Littler, Dene ^a   Celie, Patrick H N ^a   Sixma, Titia K ^a   Perrakis, Anastassis ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

^b NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

^c EUROPEAN INSTITUTE OF ONCOLOGY   (Italy)

Author keywords

Escherichia coli; Insect cells; Ligation independent cloning; Protein expression

Indexed keywords

COLD SHOCK PROTEIN; COMPLEMENTARY DNA; GENE PRODUCT; HISTIDINE; PLASMID DNA; POLYHEDRIN; POLYHISTIDINE TAG; PROTEIN; RECOMBINANT PROTEIN; UBIQUITIN; UBIQUITIN SPECIFIC PROTEASE 4; UBIQUITIN SPECIFIC PROTEASE 7; UBIQUITIN SPECIFIC PROTEASE 8; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BINDING AFFINITY; CLONING; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ESCHERICHIA COLI; EUKARYOTE; GENE CONSTRUCT; HIGH THROUGHPUT SCREENING; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SCALE UP;

ANIMALS; AUTOMATION, LABORATORY; BACULOVIRIDAE; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; ENDOPEPTIDASES; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HEXAPODA;

EID: 79959549028     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2011.03.017     Document Type: Article

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