Engineering of Thermomyces lanuginosus lipase Lip: Creation of novel biocatalyst for efficient biosynthesis of chiral intermediate of Pregabalin

Applied Microbiology and Biotechnology

Volumn 98, Issue 6, 2014, Pages 2473-2483

  Li, Xiao Jun ^a   Zheng, Ren Chao ^a   Ma, Hong Ye ^a   Zheng, Yu Guo ^a  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

(3S) 2 Carboxyethyl 3 cyano 5 methylhexanoic acid; Kinetic resolution; Lipase Lip; Pregabalin; Site saturation mutagenesis

Indexed keywords

CHEMO-ENZYMATIC SYNTHESIS; ENANTIOSELECTIVE HYDROLYSIS; ESCHERICHIA COLI CELLS; HIGH-THROUGHPUT SCREENING; KINETIC RESOLUTION; PREGABALIN; THERMOMYCES LANUGINOSUS; THERMOMYCES LANUGINOSUS LIPASE;

BIOCHEMISTRY; ESCHERICHIA COLI; MUTAGENESIS;

LOADING;

2 CARBOXYETHYL 3 CYANO 5 METHYLHEXANOIC ACID ETHYL ESTER; ANION; ESTER DERIVATIVE; HYDROLASE; OXYGEN; PREGABALIN; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

BIOENGINEERING; BIOTECHNOLOGY; CATALYST; COLIFORM BACTERIUM; DRUG; ENZYME ACTIVITY; FUNGUS; MANUFACTURING; MUTATION; REACTION KINETICS;

ARTICLE; BIOCATALYST; BIOENGINEERING; BIOSYNTHESIS; CHEMOENZYMATIC; CHIRALITY; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYMOLOGY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; HIGH THROUGHPUT SCREENING; HYDROLYSIS; MOLECULAR DOCKING; MUTAGENESIS; MUTANT; NONHUMAN; PLASMID; THERMOMYCES LANUGINOSUS; WILD TYPE;

ESCHERICHIA COLI; THERMOMYCES LANUGINOSUS;

DNA, FUNGAL; ESCHERICHIA COLI; EUROTIALES; GAMMA-AMINOBUTYRIC ACID; GENE EXPRESSION; GENETIC TESTING; LIPASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN ENGINEERING; SEQUENCE ANALYSIS, DNA;

EID: 84896698091     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5136-y     Document Type: Article

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