Aspergillus niger lipase: Heterologous expression in Pichia Pastoris, molecular modeling prediction and the importance of the hinge domains at both sides of the lid domain to interfacial activation

Biotechnology Progress

Volumn 25, Issue 2, 2009, Pages 409-416

  Shu, Zhengyu ^a,b   Duan, Mojie ^b   Yang, Jiangke ^b   Xu, Li ^b   Yan, Yunjun ^b  

^a FUJIAN NORMAL UNIVERSITY   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Aspergillus niger; Heterologous expression; Interfacial activation; Lipase; Molecular model; Pichia pastoris

Indexed keywords

ASPERGILLUS NIGER; HETEROLOGOUS EXPRESSION; INTERFACIAL ACTIVATION; MOLECULAR MODEL; PICHIA PASTORIS;

BIOCHEMICAL ENGINEERING; DICHROISM; FOOD PROCESSING; GENETIC ENGINEERING; LIGHT MEASUREMENT; MOLECULAR MECHANICS; MOLECULAR MODELING; THREE DIMENSIONAL;

LIPASES;

ASPERGILLUS NIGER; PICHIA PASTORIS;

FUNGAL PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PICHIA; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; THREE DIMENSIONAL IMAGING;

AMINO ACID SEQUENCE; ASPERGILLUS NIGER; ENZYME ACTIVATION; FUNGAL PROTEINS; GENE EXPRESSION; IMAGING, THREE-DIMENSIONAL; LIPASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT;

EID: 66149175812     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.147     Document Type: Article

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