Construction of cellobiose phosphorylase variants with broadened acceptor specificity towards anomerically substituted glucosides

Biotechnology and Bioengineering

Volumn 107, Issue 3, 2010, Pages 413-420

  De Groeve, Manu R M ^a   Remmery, Laurens ^a   Van Hoorebeke, Annelies ^a   Stout, Jan ^a   Desmet, Tom ^a   Savvides, Savvas N ^a   Soetaert, Wim ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Cellobiose phosphorylase; Cellobiosides; Directed evolution; Glycoside synthesis; Substrate specificity

Indexed keywords

CELLOBIOSE; CELLOBIOSIDES; DIRECTED EVOLUTION; GLYCOSIDE SYNTHESIS; SUBSTRATE SPECIFICITY; ACTIVE SITE; CELLULOMONAS; GENERAL APPLICATIONS; PREBIOTICS; TRIPLE MUTANTS; WILD TYPES;

PHOSPHORYLATION; SUBSTRATES; SUGARS; ENZYMES;

ENZYMES; GLYCOSIDES;

BETA GLUCOSIDASE; CELLOBIOSE; CELLOBIOSE PHOSPHORYLASE; ENZYME VARIANT; GLUCOSIDE; PREBIOTIC AGENT; UNCLASSIFIED DRUG; ALPHA GLUCOSIDASE;

ARTICLE; BINDING SITE; CELL PH; CELLULOMONAS; ENZYME SPECIFICITY; ENZYME SYNTHESIS; GENE MUTATION; HUMAN; MUTAGENESIS; MUTATION; NONHUMAN; PROTEIN EXPRESSION; WILD TYPE; PROTEIN ENGINEERING; SYNTHESIS;

AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CELLULOMONAS; GLUCOSIDES; GLUCOSYLTRANSFERASES; KINETICS; MODELS, MOLECULAR; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY;

CELLULOMONAS UDA;

EID: 78650138613     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22818     Document Type: Article

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