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Volumn 8, Issue 1, 2014, Pages 14-22

Long-term culture of primary hepatocytes: New matrices and microfluidic devices

Author keywords

Drug induced hepatotoxicity; Hydrogels; Long term culture; Matrices; Microfluidic devices; Primary hepatocytes; Scaffolds; Three dimensional culture

Indexed keywords

MOLECULAR SCAFFOLD;

EID: 84895911029     PISSN: 19360533     EISSN: 19360541     Source Type: Journal    
DOI: 10.1007/s12072-013-9487-3     Document Type: Review
Times cited : (19)

References (44)
  • 1
    • 13444284018 scopus 로고    scopus 로고
    • New technologies and screening strategies for hepatotoxicity: Use of in vitro models
    • 1:CAS:528:DC%2BD2MXhtFaqu7o%3D 15805052 10.1080/01926230590522284
    • Dambach DM, Andrews BA, Moulin F. New technologies and screening strategies for hepatotoxicity: use of in vitro models. Toxicol Pathol 2005;33:17-26
    • (2005) Toxicol Pathol , vol.33 , pp. 17-26
    • Dambach, D.M.1    Andrews, B.A.2    Moulin, F.3
  • 2
    • 84876350292 scopus 로고    scopus 로고
    • Sandwich-cultured hepatocytes: Utility for in vitro exploration of hepatobiliary drug disposition and drug-induced hepatotoxicity
    • 10.1517/17425255.2013.773973
    • De Bruyn T, Chatterjee S, Fattah S, Keemink J, Nicolai J, Augustijns P, et al. Sandwich-cultured hepatocytes: utility for in vitro exploration of hepatobiliary drug disposition and drug-induced hepatotoxicity. Expert Opin Drug Metab Toxicol 2013;5:589-616
    • (2013) Expert Opin Drug Metab Toxicol , vol.5 , pp. 589-616
    • De Bruyn, T.1    Chatterjee, S.2    Fattah, S.3    Keemink, J.4    Nicolai, J.5    Augustijns, P.6
  • 3
    • 82355193824 scopus 로고    scopus 로고
    • Human hepatocytes: Isolation, culture, and quality procedures
    • 1:CAS:528:DC%2BC38XhslWisr3K 22057448 10.1007/978-1-61779-367-7-8
    • Knobeloch D, Ehnert S, Schyschka L, Buchler P, Schoenberg M, Kleeff J, et al. Human hepatocytes: isolation, culture, and quality procedures. Methods Mol Biol 2012;806:99-120
    • (2012) Methods Mol Biol , vol.806 , pp. 99-120
    • Knobeloch, D.1    Ehnert, S.2    Schyschka, L.3    Buchler, P.4    Schoenberg, M.5    Kleeff, J.6
  • 4
    • 84883452901 scopus 로고    scopus 로고
    • Hepatic 3D cultures but not 2D cultures preserve specific transporter activity
    • 1:CAS:528:DC%2BC3sXosFymurg%3D 23728527 10.1007/s00204-013-1080-y
    • Schyschka L, Sanchez JJ, Wang Z, Burkhardt B, Muller-Vieira U, Zeilinger K, et al. Hepatic 3D cultures but not 2D cultures preserve specific transporter activity. Arch Toxicol 2013;87:1581-1593
    • (2013) Arch Toxicol , vol.87 , pp. 1581-1593
    • Schyschka, L.1    Sanchez, J.J.2    Wang, Z.3    Burkhardt, B.4    Muller-Vieira, U.5    Zeilinger, K.6
  • 5
    • 79961194518 scopus 로고    scopus 로고
    • Integrated proteomic and transcriptomic investigation of the acetaminophen toxicity in liver microfluidic biochip
    • 1:CAS:528:DC%2BC3MXhtFanur%2FI 3152546 21857903 10.1371/journal.pone. 0021268
    • Prot JM, Briffaut AS, Letourneur F, Chafey P, Merlier F, Grandvalet Y, et al. Integrated proteomic and transcriptomic investigation of the acetaminophen toxicity in liver microfluidic biochip. PLoS One 2011;6:e21268
    • (2011) PLoS One , vol.6 , pp. 21268
    • Prot, J.M.1    Briffaut, A.S.2    Letourneur, F.3    Chafey, P.4    Merlier, F.5    Grandvalet, Y.6
  • 6
    • 77952528235 scopus 로고    scopus 로고
    • Three-dimensional culture of hepatocytes for prediction of drug-induced hepatotoxicity
    • 1:CAS:528:DC%2BC3cXmtVGjt7k%3D 20380484 10.1517/17425251003674356
    • Meng Q. Three-dimensional culture of hepatocytes for prediction of drug-induced hepatotoxicity. Expert Opin Drug Metab Toxicol 2010;6:733-746
    • (2010) Expert Opin Drug Metab Toxicol , vol.6 , pp. 733-746
    • Meng, Q.1
  • 7
    • 84883464564 scopus 로고    scopus 로고
    • Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME
    • 1:CAS:528:DC%2BC3sXhtlahtr3N 3753504 23974980 10.1007/s00204-013-1078-5
    • Godoy P, Hewitt NJ, Albrecht U, Andersen ME, Ansari N, Bhattacharya S, et al. Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol 2013;87:1315-1530
    • (2013) Arch Toxicol , vol.87 , pp. 1315-1530
    • Godoy, P.1    Hewitt, N.J.2    Albrecht, U.3    Andersen, M.E.4    Ansari, N.5    Bhattacharya, S.6
  • 8
    • 33745134392 scopus 로고    scopus 로고
    • Rat hepatocyte cultures: Collagen gel sandwich and immobilization cultures
    • 1:CAS:528:DC%2BD28XhtFyqtrs%3D 16719396
    • Vinken M, Elaut G, Henkens T, Papeleu P, Snykers S, Vanhaecke T, et al. Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures. Methods Mol Biol 2006;320:247-254
    • (2006) Methods Mol Biol , vol.320 , pp. 247-254
    • Vinken, M.1    Elaut, G.2    Henkens, T.3    Papeleu, P.4    Snykers, S.5    Vanhaecke, T.6
  • 9
    • 84878479753 scopus 로고    scopus 로고
    • Characterization of a chip-based bioreactor for three-dimensional cell cultivation via magnetic resonance imaging
    • 23410914 10.1016/j.zemedi.2013.01.003
    • Gottwald E, Kleintschek T, Giselbrecht S, Truckenmuller R, Altmann B, Worgull M, et al. Characterization of a chip-based bioreactor for three-dimensional cell cultivation via magnetic resonance imaging. Z Med Phys 2013;23:102-110
    • (2013) Z Med Phys , vol.23 , pp. 102-110
    • Gottwald, E.1    Kleintschek, T.2    Giselbrecht, S.3    Truckenmuller, R.4    Altmann, B.5    Worgull, M.6
  • 10
    • 84862181007 scopus 로고    scopus 로고
    • Organotypic liver culture models: Meeting current challenges in toxicity testing
    • 1:CAS:528:DC%2BC38XosVWkurw%3D 3423873 22582993 10.3109/10408444.2012. 682115
    • LeCluyse EL, Witek RP, Andersen ME, Powers MJ. Organotypic liver culture models: meeting current challenges in toxicity testing. Crit Rev Toxicol 2012;42:501-548
    • (2012) Crit Rev Toxicol , vol.42 , pp. 501-548
    • Lecluyse, E.L.1    Witek, R.P.2    Andersen, M.E.3    Powers, M.J.4
  • 11
    • 84895908261 scopus 로고    scopus 로고
    • Synthetic polymer hydrogels for biomedical applications Chem
    • Gibas I. Janik H. Chem: Synthetic polymer hydrogels for biomedical applications; 2010
    • (2010) Janik H
    • Gibas, I.1
  • 12
    • 33646254422 scopus 로고    scopus 로고
    • Involvement of cell junctions in hepatocyte culture functionality
    • 1:CAS:528:DC%2BD28XjtlKqs7Y%3D 16809101 10.1080/10408440600599273
    • Vinken M, Papeleu P, Snykers S, De Rop E, Henkens T, Chipman JK, et al. Involvement of cell junctions in hepatocyte culture functionality. Crit Rev Toxicol 2006;36:299-318
    • (2006) Crit Rev Toxicol , vol.36 , pp. 299-318
    • Vinken, M.1    Papeleu, P.2    Snykers, S.3    De Rop, E.4    Henkens, T.5    Chipman, J.K.6
  • 13
    • 67650169752 scopus 로고    scopus 로고
    • Hydrogels as extracellular matrix mimics for 3D cell culture
    • 1:CAS:528:DC%2BD1MXnslejtb8%3D 2997742 19472329 10.1002/bit.22361
    • Tibbitt MW, Anseth KS. Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol Bioeng 2009;103:655-663
    • (2009) Biotechnol Bioeng , vol.103 , pp. 655-663
    • Tibbitt, M.W.1    Anseth, K.S.2
  • 14
    • 84864754852 scopus 로고    scopus 로고
    • A biomimetic porous hydrogel of gelatin and glycosaminoglycans cross-linked with transglutaminase and its application in the culture of hepatocytes
    • 22832766 10.1088/1748-6041/7/5/055005
    • De Colli M, Massimi M, Barbetta A, Di Rosario BL, Nardecchia S, Conti Devirgiliis L, et al. A biomimetic porous hydrogel of gelatin and glycosaminoglycans cross-linked with transglutaminase and its application in the culture of hepatocytes. Biomed Mater 2012;7:055005
    • (2012) Biomed Mater , vol.7 , pp. 055005
    • De Colli, M.1    Massimi, M.2    Barbetta, A.3    Di Rosario, B.L.4    Nardecchia, S.5    Conti Devirgiliis, L.6
  • 15
    • 0030070103 scopus 로고    scopus 로고
    • Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function
    • 1:CAS:528:DyaK28XovVSmtQ%3D%3D 8745335 10.1016/0142-9612(96)85576-1
    • Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials 1996;17:373-385
    • (1996) Biomaterials , vol.17 , pp. 373-385
    • Moghe, P.V.1    Berthiaume, F.2    Ezzell, R.M.3    Toner, M.4    Tompkins, R.G.5    Yarmush, M.L.6
  • 16
    • 0034964158 scopus 로고    scopus 로고
    • Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation
    • 1:CAS:528:DC%2BD3MXkt1yisLY%3D 11408150 10.1016/S0928-0987(01)00135-X
    • LeCluyse EL. Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation. Eur J Pharm Sci 2001;13:343-368
    • (2001) Eur J Pharm Sci , vol.13 , pp. 343-368
    • Lecluyse, E.L.1
  • 17
    • 0035137478 scopus 로고    scopus 로고
    • Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function
    • 1:CAS:528:DC%2BD3MXhtF2nsrw%3D 11211910 10.1016/S0168-8278(00)00080-5
    • Khalil M, Shariat-Panahi A, Tootle R, Ryder T, McCloskey P, Roberts E, et al. Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function. J Hepatol 2001;34:68-77
    • (2001) J Hepatol , vol.34 , pp. 68-77
    • Khalil, M.1    Shariat-Panahi, A.2    Tootle, R.3    Ryder, T.4    McCloskey, P.5    Roberts, E.6
  • 18
    • 33746078100 scopus 로고    scopus 로고
    • Acetaminophen-induced hepatotoxicity of gel entrapped rat hepatocytes in hollow fibers
    • 1:CAS:528:DC%2BD28XntlWgtLY%3D 16797510 10.1016/j.cbi.2006.05.005
    • Shen C, Zhang G, Qiu H, Meng Q. Acetaminophen-induced hepatotoxicity of gel entrapped rat hepatocytes in hollow fibers. Chem Biol Interact 2006;162:53-61
    • (2006) Chem Biol Interact , vol.162 , pp. 53-61
    • Shen, C.1    Zhang, G.2    Qiu, H.3    Meng, Q.4
  • 19
    • 33747792067 scopus 로고    scopus 로고
    • Sensitivities of gel entrapped hepatocytes in hollow fibers to hepatotoxic drug
    • 1:CAS:528:DC%2BD28Xosl2lu70%3D 16814964 10.1016/j.toxlet.2006.05.008
    • Meng Q, Zhang G, Shen C, Qiu H. Sensitivities of gel entrapped hepatocytes in hollow fibers to hepatotoxic drug. Toxicol Lett 2006;166:19-26
    • (2006) Toxicol Lett , vol.166 , pp. 19-26
    • Meng, Q.1    Zhang, G.2    Shen, C.3    Qiu, H.4
  • 20
    • 78650551465 scopus 로고    scopus 로고
    • Hydrophobic nanoparticles improve permeability of cell-encapsulating poly(ethylene glycol) hydrogels while maintaining patternability
    • 1:CAS:528:DC%2BC3cXhsFeisrvO 2996449 21071674 10.1073/pnas.1005211107
    • Lee W, Cho NJ, Xiong A, Glenn JS, Frank CW. Hydrophobic nanoparticles improve permeability of cell-encapsulating poly(ethylene glycol) hydrogels while maintaining patternability. Proc Natl Acad Sci U S A 2010;107:20709-20714
    • (2010) Proc Natl Acad Sci U S A , vol.107 , pp. 20709-20714
    • Lee, W.1    Cho, N.J.2    Xiong, A.3    Glenn, J.S.4    Frank, C.W.5
  • 21
    • 77549088407 scopus 로고    scopus 로고
    • Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes
    • 1:CAS:528:DC%2BC3cXisFSlsbc%3D 2837121 20153045 10.1016/j.biomaterials. 2010.01.068
    • Kim M, Lee JY, Jones CN, Revzin A, Tae G. Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes. Biomaterials 2010;31:3596-3603
    • (2010) Biomaterials , vol.31 , pp. 3596-3603
    • Kim, M.1    Lee, J.Y.2    Jones, C.N.3    Revzin, A.4    Tae, G.5
  • 22
    • 60149111910 scopus 로고    scopus 로고
    • PEG hydrogels for the controlled release of biomolecules in regenerative medicine
    • 19089601 10.1007/s11095-008-9801-2
    • Lin CC, Anseth KS. PEG hydrogels for the controlled release of biomolecules in regenerative medicine. Pharm Res 2008;26:631-643
    • (2008) Pharm Res , vol.26 , pp. 631-643
    • Lin, C.C.1    Anseth, K.S.2
  • 23
    • 77449103100 scopus 로고    scopus 로고
    • Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro
    • 19912437 10.1111/j.1582-4934.2009.00970.x
    • Genove E, Schmitmeier S, Sala A, Borros S, Bader A, Griffith LG, et al. Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro. J Cell Mol Med 2009;13:3387-3397
    • (2009) J Cell Mol Med , vol.13 , pp. 3387-3397
    • Genove, E.1    Schmitmeier, S.2    Sala, A.3    Borros, S.4    Bader, A.5    Griffith, L.G.6
  • 24
    • 39449138696 scopus 로고    scopus 로고
    • Three-dimensional primary hepatocyte culture in synthetic self-assembling peptide hydrogel
    • 1:CAS:528:DC%2BD1cXjsFCmu74%3D 18333775 10.1089/tea.2007.0143
    • Wang S, Nagrath D, Chen PC, Berthiaume F, Yarmush ML. Three-dimensional primary hepatocyte culture in synthetic self-assembling peptide hydrogel. Tissue Eng Part A 2008;14:227-236
    • (2008) Tissue Eng Part A , vol.14 , pp. 227-236
    • Wang, S.1    Nagrath, D.2    Chen, P.C.3    Berthiaume, F.4    Yarmush, M.L.5
  • 25
    • 0346367111 scopus 로고    scopus 로고
    • Culture of primary rat hepatocytes within porous chitosan scaffolds
    • 14613242 10.1002/jbm.a.10076
    • Li J, Pan J, Zhang L, Guo X, Yu Y. Culture of primary rat hepatocytes within porous chitosan scaffolds. J Biomed Mater Res A 2003;67:938-943
    • (2003) J Biomed Mater Res A , vol.67 , pp. 938-943
    • Li, J.1    Pan, J.2    Zhang, L.3    Guo, X.4    Yu, Y.5
  • 26
    • 0041859380 scopus 로고    scopus 로고
    • Liver tissue engineering within alginate scaffolds: Effects of cell-seeding density on hepatocyte viability, morphology, and function
    • 1:CAS:528:DC%2BD3sXmvVOnu74%3D 13678452 10.1089/107632703768247430
    • Dvir-Ginzberg M, Gamlieli-Bonshtein I, Agbaria R, Cohen S. Liver tissue engineering within alginate scaffolds: effects of cell-seeding density on hepatocyte viability, morphology, and function. Tissue Eng 2003;9:757-766
    • (2003) Tissue Eng , vol.9 , pp. 757-766
    • Dvir-Ginzberg, M.1    Gamlieli-Bonshtein, I.2    Agbaria, R.3    Cohen, S.4
  • 27
    • 3342921892 scopus 로고    scopus 로고
    • Chitosan/gelatin composite microcarrier for hepatocyte culture
    • 1:CAS:528:DC%2BD2cXkvVyktLw%3D 15269534 10.1023/B:bile.0000025896.61490. 6d
    • Li K, Wang Y, Miao Z, Xu D, Tang Y, Feng M. Chitosan/gelatin composite microcarrier for hepatocyte culture. Biotechnol Lett 2004;26:879-883
    • (2004) Biotechnol Lett , vol.26 , pp. 879-883
    • Li, K.1    Wang, Y.2    Miao, Z.3    Xu, D.4    Tang, Y.5    Feng, M.6
  • 28
    • 0037707222 scopus 로고    scopus 로고
    • Effects of three-dimensional scaffolds on cell organization and tissue development
    • 1:CAS:528:DC%2BD3MXotFKisLw%3D 10.1007/BF02932999
    • Li Y, Yang S-T. Effects of three-dimensional scaffolds on cell organization and tissue development. Biotech Bioprocess Eng 2001;6:311-325
    • (2001) Biotech Bioprocess Eng , vol.6 , pp. 311-325
    • Li, Y.1    Yang, S.-T.2
  • 29
    • 0345600939 scopus 로고    scopus 로고
    • Polyvinylalcohol three-dimensional matrices for improved long-term dynamic cultureof hepatocytes
    • Saavedra YGL, Mateescu MA, Averill-Bates DA, Denizeau F. Polyvinylalcohol three-dimensional matrices for improved long-term dynamic cultureof hepatocytes. J Biomed Mater Res A 2002;66:562-570
    • (2002) J Biomed Mater Res A , vol.66 , pp. 562-570
    • Saavedra, Y.G.L.1    Mateescu, M.A.2    Averill-Bates, D.A.3    Denizeau, F.4
  • 30
    • 79551504497 scopus 로고    scopus 로고
    • Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: A promising model for improving transplantation efficiency with tissue engineering
    • 21280182 10.1002/lt.22200
    • Torok E, Lutgehetmann M, Bierwolf J, Melbeck S, Dullmann J, Nashan B, et al. Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering. Liver Transpl 2011;17:104-114
    • (2011) Liver Transpl , vol.17 , pp. 104-114
    • Torok, E.1    Lutgehetmann, M.2    Bierwolf, J.3    Melbeck, S.4    Dullmann, J.5    Nashan, B.6
  • 31
    • 84874869084 scopus 로고    scopus 로고
    • Nanoporous fibers of type-I collagen coated poly(l-lactic acid) for enhancing primary hepatocyte growth and function
    • 1:CAS:528:DC%2BC38XhvVCisrnP 10.1039/c2tb00195k
    • Wang T, Feng Z, Leach M, Wu J, Jiang Q. Nanoporous fibers of type-I collagen coated poly(l-lactic acid) for enhancing primary hepatocyte growth and function. J Mat Chem B 2013;1:339-346
    • (2013) J Mat Chem B , vol.1 , pp. 339-346
    • Wang, T.1    Feng, Z.2    Leach, M.3    Wu, J.4    Jiang, Q.5
  • 32
    • 80053360260 scopus 로고    scopus 로고
    • Rat primary hepatocytes show enhanced performance and sensitivity to acetaminophen during three-dimensional culture on a polystyrene scaffold designed for routine use
    • 1:CAS:528:DC%2BC3MXht1Cht7bF 21675871 10.1089/adt.2011.0371
    • Schutte M, Fox B, Baradez MO, Devonshire A, Minguez J, Bokhari M, et al. Rat primary hepatocytes show enhanced performance and sensitivity to acetaminophen during three-dimensional culture on a polystyrene scaffold designed for routine use. Assay Drug Dev Technol 2011;9:475-486
    • (2011) Assay Drug Dev Technol , vol.9 , pp. 475-486
    • Schutte, M.1    Fox, B.2    Baradez, M.O.3    Devonshire, A.4    Minguez, J.5    Bokhari, M.6
  • 33
    • 84872301011 scopus 로고    scopus 로고
    • Multi-cell type human liver microtissues for hepatotoxicity testing
    • 1:CAS:528:DC%2BC38Xhs1Cqtr%2FE 3535351 23143619 10.1007/s00204-012-0968-2
    • Messner S, Agarkova I, Moritz W, Kelm JM. Multi-cell type human liver microtissues for hepatotoxicity testing. Arch Toxicol 2013;87:209-213
    • (2013) Arch Toxicol , vol.87 , pp. 209-213
    • Messner, S.1    Agarkova, I.2    Moritz, W.3    Kelm, J.M.4
  • 34
    • 80051772114 scopus 로고    scopus 로고
    • Microfabricated polyester conical microwells for cell culture applications
    • 1:CAS:528:DC%2BC3MXotFSltbs%3D 3319023 21614380 10.1039/c1lc20213h
    • Selimovic S, Piraino F, Bae H, Rasponi M, Redaelli A, Khademhosseini A. Microfabricated polyester conical microwells for cell culture applications. Lab Chip 2011;11:2325-2332
    • (2011) Lab Chip , vol.11 , pp. 2325-2332
    • Selimovic, S.1    Piraino, F.2    Bae, H.3    Rasponi, M.4    Redaelli, A.5    Khademhosseini, A.6
  • 35
    • 79955489141 scopus 로고    scopus 로고
    • Modular bioreactor for primary human hepatocyte culture: Medium flow stimulates expression and activity of detoxification genes
    • 1:CAS:528:DC%2BC3MXlsVOhs74%3D 3123466 21259441 10.1002/biot.201000326
    • Vinci B, Duret C, Klieber S, Gerbal-Chaloin S, Sa-Cunha A, Laporte S, et al. Modular bioreactor for primary human hepatocyte culture: medium flow stimulates expression and activity of detoxification genes. Biotechnol J 2011;6:554-564
    • (2011) Biotechnol J , vol.6 , pp. 554-564
    • Vinci, B.1    Duret, C.2    Klieber, S.3    Gerbal-Chaloin, S.4    Sa-Cunha, A.5    Laporte, S.6
  • 36
    • 78649644415 scopus 로고    scopus 로고
    • Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors
    • 1:CAS:528:DC%2BC3cXhsVyms7vN 20184401 10.1089/ten.tec.2009.0784
    • Miranda JP, Rodrigues A, Tostoes RM, Leite S, Zimmerman H, Carrondo MJ, et al. Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors. Tissue Eng Part C Methods 2010;16:1223-1232
    • (2010) Tissue Eng Part C Methods , vol.16 , pp. 1223-1232
    • Miranda, J.P.1    Rodrigues, A.2    Tostoes, R.M.3    Leite, S.4    Zimmerman, H.5    Carrondo, M.J.6
  • 37
    • 84868126148 scopus 로고    scopus 로고
    • Analysis of drug metabolism activities in a miniaturized liver cell bioreactor for use in pharmacological studies
    • 1:CAS:528:DC%2BC38XptFCqsrY%3D 22688505 10.1002/bit.24573
    • Hoffmann SA, Muller-Vieira U, Biemel K, Knobeloch D, Heydel S, Lubberstedt M, et al. Analysis of drug metabolism activities in a miniaturized liver cell bioreactor for use in pharmacological studies. Biotechnol Bioeng 2012;109:3172-3181
    • (2012) Biotechnol Bioeng , vol.109 , pp. 3172-3181
    • Hoffmann, S.A.1    Muller-Vieira, U.2    Biemel, K.3    Knobeloch, D.4    Heydel, S.5    Lubberstedt, M.6
  • 38
    • 84941599468 scopus 로고    scopus 로고
    • Serum-free culture of primary human hepatocytes in a miniaturized hollow-fibre membrane bioreactor for pharmacological in vitro studies
    • doi: 10.1002/term.1652
    • Lubberstedt M, Muller-Vieira U, Biemel KM, Darnell M, Hoffmann SA, Knospel F, et al. Serum-free culture of primary human hepatocytes in a miniaturized hollow-fibre membrane bioreactor for pharmacological in vitro studies. J Tissue Eng Regen Med 2012. doi: 10.1002/term.1652
    • (2012) J Tissue Eng Regen Med
    • Lubberstedt, M.1    Muller-Vieira, U.2    Biemel, K.M.3    Darnell, M.4    Hoffmann, S.A.5    Knospel, F.6
  • 39
    • 79960562298 scopus 로고    scopus 로고
    • In-depth physiological characterization of primary human hepatocytes in a 3D hollow-fiber bioreactor
    • 1:CAS:528:DC%2BC3MXhtVejtLjM 21442764 10.1002/term.418
    • Mueller D, Tascher G, Muller-Vieira U, Knobeloch D, Nuessler AK, Zeilinger K, et al. In-depth physiological characterization of primary human hepatocytes in a 3D hollow-fiber bioreactor. J Tissue Eng Regen Med 2011;5:e207-e218
    • (2011) J Tissue Eng Regen Med , vol.5
    • Mueller, D.1    Tascher, G.2    Muller-Vieira, U.3    Knobeloch, D.4    Nuessler, A.K.5    Zeilinger, K.6
  • 40
    • 0034980269 scopus 로고    scopus 로고
    • Adsorption of xenobiotics to plastic tubing incorporated into dynamic in vitro systems used in pharmacological research - Limits and progress
    • 1:CAS:528:DC%2BD3MXksVygtbk%3D 11426882 10.1016/S0142-9612(00)00389-6
    • Unger JK, Kuehlein G, Schroers A, Gerlach JC, Rossaint R. Adsorption of xenobiotics to plastic tubing incorporated into dynamic in vitro systems used in pharmacological research - limits and progress. Biomaterials 2001;22:2031-2037
    • (2001) Biomaterials , vol.22 , pp. 2031-2037
    • Unger, J.K.1    Kuehlein, G.2    Schroers, A.3    Gerlach, J.C.4    Rossaint, R.5
  • 41
    • 1442300210 scopus 로고    scopus 로고
    • The optimal hepatocyte density for a hollow-fiber bioartificial liver
    • 1:CAS:528:DC%2BD2cXislyju7o%3D 15038673
    • Xu Q, Sun X, Qiu Y, Zhang H, Ding Y. The optimal hepatocyte density for a hollow-fiber bioartificial liver. Ann Clin Lab Sci 2004;34:87-93
    • (2004) Ann Clin Lab Sci , vol.34 , pp. 87-93
    • Xu, Q.1    Sun, X.2    Qiu, Y.3    Zhang, H.4    Ding, Y.5
  • 42
    • 27944442381 scopus 로고    scopus 로고
    • A microscale in vitro physiological model of the liver: Predictive screens for drug metabolism and enzyme induction
    • 1:CAS:528:DC%2BD2MXht1GrurvM 16379670 10.2174/138920005774832632
    • Sivaraman A, Leach JK, Townsend S, Iida T, Hogan BJ, Stolz DB, et al. A microscale in vitro physiological model of the liver: predictive screens for drug metabolism and enzyme induction. Curr Drug Metab 2005;6:569-691
    • (2005) Curr Drug Metab , vol.6 , pp. 569-591
    • Sivaraman, A.1    Leach, J.K.2    Townsend, S.3    Iida, T.4    Hogan, B.J.5    Stolz, D.B.6
  • 43
    • 26444567846 scopus 로고    scopus 로고
    • Microstructured scaffolds for liver tissue cultures of high cell density: Morphological and biochemical characterization of tissue aggregates
    • 1:CAS:528:DC%2BD2MXktFKjs7s%3D 15770659 10.1002/jcb.20360
    • Eschbach E, Chatterjee SS, Noldner M, Gottwald E, Dertinger H, Weibezahn KF, et al. Microstructured scaffolds for liver tissue cultures of high cell density: morphological and biochemical characterization of tissue aggregates. J Cell Biochem 2005;95:243-255
    • (2005) J Cell Biochem , vol.95 , pp. 243-255
    • Eschbach, E.1    Chatterjee, S.S.2    Noldner, M.3    Gottwald, E.4    Dertinger, H.5    Weibezahn, K.F.6
  • 44
    • 79958113646 scopus 로고    scopus 로고
    • Artificial micro organs" - A microfluidic device for dielectrophoretic assembly of liver sinusoids
    • 21347825 10.1007/s10544-011-9517-7
    • Schutte J, Hagmeyer B, Holzner F, Kubon M, Werner S, Freudigmann C, et al. "Artificial micro organs" - a microfluidic device for dielectrophoretic assembly of liver sinusoids. Biomed Microdevices 2011;13:493-501
    • (2011) Biomed Microdevices , vol.13 , pp. 493-501
    • Schutte, J.1    Hagmeyer, B.2    Holzner, F.3    Kubon, M.4    Werner, S.5    Freudigmann, C.6


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