Microstructured scaffolds for liver tissue cultures of high cell density: Morphological and biochemical characterization of tissue aggregates

Journal of Cellular Biochemistry

Volumn 95, Issue 2, 2005, Pages 243-255

  Eschbach, Erik ^a   Chatterjee, Shyam S ^b   Nöldner, Michael ^b   Gottwald, Eric ^a   Dertinger, Hermann ^a   Weibezahn, Karl Friedrich ^a   Knedlitschek, Gudrun ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

^b DR WILLMAR SCHWABE GMBH AND CO KG   (Germany)

Author keywords

Albumin secretion; Bioreactor; Cytochrome P450; Extracorporeal liver; Mixed function oxydases; Rat hepatocytes; Three dimensional cell culture; Transplantation

Indexed keywords

7 BENZYLOXYRESORUFIN O DEBENZYLASE; 7 ETHOXYCOUMARIN DEETHYLASE; ALBUMIN; CYTOCHROME P450; ETHOXYRESORUFIN DEETHYLASE; MIXED FUNCTION OXIDASE; PENTOXYRESORUFIN DEALKYLASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DENSITY; CELL DIFFERENTIATION; CELL ISOLATION; CELL METABOLISM; CELL SPECIFICITY; CELL STRUCTURE; CELL VACUOLE; CELL VOLUME; EXTRACELLULAR MATRIX; IN VITRO STUDY; LIVER; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN SECRETION; RAT; TISSUE CULTURE; TISSUE DIFFERENTIATION; VALIDATION PROCESS; XENOBIOTIC METABOLISM;

ACTINS; ALBUMINS; ANIMALS; CELLS, CULTURED; COUMARINS; CULTURE MEDIA; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX; FRUCTOSE-BISPHOSPHATE ALDOLASE; HEPATOCYTES; MALE; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; TYROSINE TRANSAMINASE; XENOBIOTICS;

EID: 26444567846     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcb.20360     Document Type: Article

References (40)

1. Acosta D, Anuforo DC, McMillin R, Soine WH, Smith RV. 1979. Comparison of cytochrome P-450 levels in adult rat liver, postnatal rat liver, and primary cultures of post-natal rat hepatocytes. Life Sci 25:1413-1418.
2. Akrawi M, Rogiers V, Vandenberghe Y, Palmer CN, Vercruysse A, Shephard EA, Phillips IR. 1993. Maintenance and induction in co-cultured rat hepatocytes of components of the cytochrome P450-mediated mono-oxygenase. Biochem Pharmacol 45:1583-1591.
3. Bader A, Knop E, Kern A, Boker K, Fruhauf N, Crome O, Esselmann H, Pape C, Kempka G, Sewing KF. 1996. 3-D coculture of hepatic sinusoidal cells with primary hepatocytes-design of an organotypical model. Exp Cell Res 226:223-233.
4. Berry MN, Friend DS. 1969. High-yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study. J Cell Biol 43:506-520.
5. Bhatia SN, Balis UJ, Yarmush ML, Toner M. 1999. Effect of cell-cell interactions in preservation of cellular phenotype: Cocultivation of hepatocytes and nonparenchymal cells. Faseb J 13:1883-1900.
6. Corlu A, Kneip B, Lhadi C, Leray G, Glaise D, Baffet G, Bourel D, Guguen-Guillouzo C. 1991. A plasma membrane protein is involved in cell contact-mediated regulation of tissue-specific genes in adult hepatocytes. J Cell Biol 115:505-515.
7. Dich J, Vind C, Grunnet N. 1988. Long-term culture of hepatocytes: Effect of hormones on enzyme activities and metabolic capacity. Hepatology 8:39-45.
8. Donato MT, Castell JV, Gomez-Lechon MJ. 1994. Cytochrome P450 activities in pure and co-cultured rat hepatocytes. Effects of model inducers. In vitro Cell Dev Biol Anim 30A:825-832.
9. Enat R, Jefferson DM, Ruiz-Opazo N, Gatmaitan Z, Leinwand LA, Reid LM. 1984. Hepatocyte proliferation in vitro: Its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix. Proc Natl Acad Sci USA 81:1411-1415.
10. Flaim KE, Hutson SM, Lloyd CE, Taylor JM, Shiman R, Jefferson LS. 1985. Direct effect of insulin on albumin gene expression in primary cultures of rat hepatocytes. Am J Physiol 249:E447-E453.
11. Fraslin JM, Kneip B, Vaulont S, Glaise D, Munnich A, Guguen-Guillouzo C. 1985. Dependence of hepatocyte-specific gene expression on cell-cell interactions in primary culture. Embo J 4:2487-2491.
12. Fujita M, Spray DC, Choi H, Saez JC, Watanabe T, Rosenberg LC, Hertzberg EL, Reid LM. 1987. Glycosaminoglycans and proteoglycans induce gap junction expression and restore transcription of tissue-specific mRNAs in primary liver cultures. Hepatology 7:1S-9S.
13. Gomez-Lechon MJ, Montoya A, Lopez P, Donato T, Larrauri A, Castell JV. 1988. The potential use of cultured hepatocytes in predicting the hepatotoxicity of xenobiotics. Xenobiotica 18:725-735.
14. Goulet F, Normand C, Morin O. 1988. Cellular interactions promote tissue-specific function, biomatrix deposition and junctional communication of primary cultured hepatocytes. Hepatology 8:1010-1018.
15. Groebe K, Mueller-Klieser W. 1996. On the relation between size of necrosis and diameter of tumor spheroids, Int J Radiat Oncol Biol Phys 34:395-401.
16. Guguen-Guillouzo C, Gripon P, Vandenberghe Y, Lamballe F, Ratanasavanh D, Guillouzo A. 1988. Hepatotoxicity and molecular aspects of hepatocyte function in primary culture. Xenobiotica 18:773-783.
17. Hutson SM, Stinson-Fisher C, Shiman R, Jefferson LS. 1987. Regulation of albumin synthesis by hormones and amino acids in primary cultures of rat hepatocytes. Am J Physiol 252:E291-E298.
18. Jefferson DM, Clayton DF, Darnell JE, Jr., Reid LM. 1984. Posttranscriptional modulation of gene expression in cultured rat hepatocytes. Mol Cell Biol 4:1929-1934.
19. Jefferson DM, Reid LM, Giambrone MA, Shafritz DA, Zern MA. 1985. Effects of dexamethasone on albumin and collagen gene expression in primary cultures of adult rat hepatocytes. Hepatology 5:14-20.
20. Knedlitschek G, Schneider F, Gottwald E, Schaller T, Eschbach E, Weibezahn KF. 1999. A tissue-like culture system using microstructures: Influence of extracellular matrix material on cell adhesion and aggregation. J Biomech Eng 121:35-39.
21. Koebe HG, Pahernik S, Eyer P, Schildberg FW. 1994. Collagen gel immobilization: A useful cell culture technique for long-term metabolic studies on human hepatocytes. Xenobiotica 24:95-107.
22. Koide N, Sakaguchi K, Koide Y, Asano K, Kawaguchi M, Matsushima H, Takenami T, Shinji T, Mori M, Tsuji T. 1990. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp Cell Res 186:227-235.
23. Lake BG. 1987. Preparation and characterisation of microsomal fractions for studies on xenobiotic metabolism. In: Snell K, Mullock B, editors. Biochemical toxicology. Oxford, Washington: IRL Presss. pp 183-215.
24. Landry J, Bernier D, Ouellet C, Goyette R, Marceau N. 1985. Spheroidal aggregate culture of rat liver cells: Histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J Cell Biol 101: 914-923.
25. Ma M, Xu J, Purcell WM. 2003. Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: I. morphological maturation and kinetic changes of energy metabolism, albumin synthesis, and activities of some enzymes. J Cell Biochem 90:1166-1175.
26. Michalopoulos G, Pitot HC. 1975. Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations. Exp Cell Res 94:70-78.
27. Morin O, Normand C. 1986. Long-term maintenance of hepatocyte functional activity in co-culture: Requirements for sinusoidal endothelial cells and dexamethasone. J Cell Physiol 129:103-110.
28. Muller-Enoch D, Buttgen E, Nonnenmacher A. 1985. Regioselective O-demethylation of scoparone: Differentiation between rat liver cytochrome P-450 isozymes. Z Naturforsch [C] 40:682-684.
29. Nawa K, Nakamura T, Kumatori A, Noda C, Ichihara A. 1986. Glucocorticoid-dependent expression of the albumin gene in adult rat hepatocytes. J Biol Chem 261:16883-16888.
30. Omura T, Sato R. 1964. The carbon monoxide-binding pigment of liver microsomes 1. Evidence for its hemoprotein nature. J Biol Chem 239:2370-2377.
31. Saad B, Schawalder H, Maier P. 1993. Crude liver membrane fractions as substrate preserve liver-specific functions in long-term, serum-free rat hepatocyte cultures. In vitro Cell Dev Biol 29A:32-40.
32. Sambrook J, Fritsch E, Maniatis T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor laboratory press.
33. Seglen PO. 1973. Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion. Exp Cell Res 82:391-398.
34. Spray DC, Fujita M, Saez JC, Choi H, Watanabe T, Hertzberg E, Rosenberg LC, Reid LM. 1987. Proteoglycans and glycosaminoglycans induce gap junction synthesis and function in primary liver cultures. J Cell Biol 105:541-551.
35. Tong JZ, De Lagausie P, Furlan V, Cresteil T, Bernard O, Alvarez F. 1992. Long-term culture of adult rat hepatocyte spheroids. Exp Cell Res 200:326-332.
36. Walser B, Drosselmeyer E, Weibezahn KF. 1989. Three-dimensional culture systems for rat hepatocytes (spheroids). Eur J Cell Biol 48:71.
37. Walser B, Wildeboer B, Weibezahn KF, Dertinger H. 1991. Metabolic properties of adult rat hepatocytes cultured as multicellular spheroids. Eur J Cell Biol 54:81.
38. Weibezahn KF, Knedlitschek G, Bier W, Schaller T. 1994. Mechanically processed microstructures used to establish an in vitro tissue model. In: Reichl H, Heuberger A, editors. Micro System Technologies, Vol. 94. Berlin: VDE-Verlag. pp 873-878.
39. Weibezahn KF, Knedlitschek G, Dertinger H, Bier W, Schaller T, Schubert K. 1995. Reconstruction of tissue layers in mechanically processed microstructures. J Exp Clinic Cancer Res Suppl 14:41-42.
40. Xu J, Ma M, Purcell WM. 2003. Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: II. Nitric oxide synthesis and related changes. J Cell Biochem 90:1176-1185.