PEG hydrogels for the controlled release of biomolecules in regenerative medicine

Pharmaceutical Research

Volumn 26, Issue 3, 2009, Pages 631-643

  Lin, C C ^a,b   Anseth, K S ^a,b  

^a UCB 450   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Controlled release; Hydrogel; PEG; Regenerative medicine

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR; MACROGOL; OLIGO[POLY(ETHYLENE GLYCOL)FUMARATE]; POLY(PROPYLENE FUMARATE CO ETHYLENE GLYCOL); POLYGLACTIN; POLYLACTIC ACID; UNCLASSIFIED DRUG; VASCULOTROPIN;

BIOCOMPATIBILITY; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED RELEASE FORMULATION; DRUG DELIVERY SYSTEM; DRUG STABILITY; GELATION; HUMAN; HYDROGEL; POLYMERIZATION; PRIORITY JOURNAL; REVIEW; STEM CELL;

ANIMALS; BIOCOMPATIBLE MATERIALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA; DELAYED-ACTION PREPARATIONS; DRUG DESIGN; HUMANS; HYDROGELS; MACROMOLECULAR SUBSTANCES; POLYETHYLENE GLYCOLS; REGENERATIVE MEDICINE; STEM CELLS;

EID: 60149111910     PISSN: 07248741     EISSN: 1573904X     Source Type: Journal    
DOI: 10.1007/s11095-008-9801-2     Document Type: Review

References (124)

1. M. P. Lutolf J. A. Hubbell 2005 Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering Nat. Biotechnol. 23 47 55
2. C. C. Lin A. T. Metters 2006 Hydrogels in controlled release formulations: Network design and mathematical modeling Adv. Drug Deliv. Rev. 58 1379 1408
3. N. A. Peppas P. Bures W. Leobandung H. Ichikawa 2000 Hydrogels in pharmaceutical formulations Eur. J. Pharm. Biopharm. 50 27 46
4. N. A. Peppas J. Z. Hilt A. Khademhosseini R. Langer 2006 Hydrogels in biology and medicine: From molecular principles to bionanotechnology Adv. Mater. 18 1345 1360
5. J. L. Drury D. J. Mooney 2003 Hydrogels for tissue engineering: scaffold design variables and applications Biomaterials 24 4337 4351
6. M. B. Mellott K. Searcy M. V. Pishko 2001 Release of protein from highly cross-linked hydrogels of poly(ethylene glycol) diacrylate fabricated by UV polymerization Biomaterials 22 929 941
7. N. A. Peppas K. B. Keys M. Torres-Lugo A. M. Lowman 1999 Poly(ethylene glycol)-containing hydrogels in drug delivery J. Control. Release 62 81 87
8. C. R. Nuttelman M. A. Rice A. E. Rydholm C. N. Salinas D. N. Shah K. S. Anseth 2008 Macromolecular monomers for the synthesis of hydrogel niches and their application in cell encapsulation and tissue engineering Prog. Polym. Sci. 33 167 179
9. S. J. Bryant C. R. Nuttelman K. S. Anseth 2000 Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro J. Biomater. Sci. Polym. Ed. 11 439 457
10. J. A. Burdick K. S. Anseth 2002 Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering Biomaterials 23 4315 4323
11. A. K. Pavlou J. M. Reichert 2004 Recombinant protein therapeutics-success rates, market trends and values to 2010 Nat. Biotechnol. 22 1513 1519
12. K. A. Davis K. S. Anseth 2002 Controlled release from crosslinked degradable networks Crit. Rev. Ther. Drug Carr. Syst. 19 385 423
13. A. E. Rydholm C. N. Bowman K. S. Anseth 2005 Degradable thiol-acrylate photopolymers: polymerization and degradation behavior of an in situ forming biomaterial Biomaterials 26 4495 4506
14. E. Fournier C. Passirani C. N. Montero-Menei J. P. Benoit 2003 Biocompatibility of implantable synthetic polymeric drug carriers: focus on brain biocompatibility Biomaterials 24 3311 3331
15. A. T. Metters K. S. Anseth C. N. Bowman 2001 A statistical kinetic model for the bulk degradation of PLA-b-PEG-b-PLA hydrogel networks: Incorporating network non-idealities J. Phys. Chem. B 105 8069 8076
16. G. Odian. Principles of polymerization. Wiley, New Jersey, 2004.
17. M. Malkoch, R. Vestberg, N. Gupta, L. Mespouille, P. Dubois, A. F. Mason, J. L. Hedrick, Q. Liao, C. W. Frank, K. Kingsbury, and C. J. Hawker. Synthesis of well-defined hydrogel networks using Click chemistry. Chem. Commun. 2774-2776 (2006). doi: 10.1039/b603438a.
18. S. C. Rizzi M. Ehrbar S. Halstenberg G. P. Raeber H. G. Schmoekel H. Hagenmuller R. Muller F. E. Weber J. A. Hubbell 2006 Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part II: Biofunctional characteristics Biomacromolecules 7 3019 3029
19. S. C. Rizzi J. A. Hubbell 2005 Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part 1: Development and physicochernical characteristics Biomacromolecules 6 1226 1238
20. D. L. Elbert A. B. Pratt M. P. Lutolf S. Halstenberg J. A. Hubbell 2001 Protein delivery from materials formed by self-selective conjugate addition reactions J. Control. Release 76 11 25
21. P. van de Wetering A. T. Metters R. G. Schoenmakers J. A. Hubbell 2005 Poly(ethylene glycol) hydrogels formed by conjugate addition with controllable swelling, degradation, and release of pharmaceutically active proteins J. Control. Release 102 619 627
22. T. P. Kraehenbuehl P. Zammaretti A. J. Van der Vlies R. G. Schoenmakers M. P. Lutolf M. E. Jaconi J. A. Hubbell 2008 Three-dimensional extracellular matrix-directed cardioprogenitor differentiation: systematic modulation of a synthetic cell-responsive PEG-hydrogel Biomaterials 29 2757 2766
23. G. P. Raeber M. P. Lutolf J. A. Hubbell 2005 Molecularly engineered PEG hydrogels: a novel model system for proteolytically mediated cell migration Biophys. J. 89 1374 1388
24. H. C. Kolb M. G. Finn K. B. Sharpless 2001 Click chemistry: diverse chemical function from a few good reactions Angew. Chem. Int. Ed 40 2004 2020
25. V. D. Bock, H. Hiemstra, and J. H. van Maarseveen. Cu-I-catalyzed alkyne-azide "click" cycloadditions from a mechanistic and synthetic perspective. Eur. J. Org. Chem. 51-68 (2005).
26. B. D. Polizzotti B. D. Fairbanks K. S. Anseth 2008 Three-dimensional biochemical patterning of click-based composite hydrogels via thiolene photopolymerization Biomacromolecules 9 1084 1087
27. J. M. Baskin J. A. Prescher S. T. Laughlin N. J. Agard P. V. Chang I. A. Miller A. Lo J. A. Codelli C. R. Bertozzit 2007 Copper-free click chemistry for dynamic in vivo imaging Proc. Natl. Acad. Sci. U.S.A. 104 16793 16797
28. S. T. Laughlin J. M. Baskin S. L. Amacher C. R. Bertozzi 2008 In vivo imaging of membrane-associated glycans in developing zebrafish Science 320 664 667
29. A. E. Rydholm S. K. Reddy K. S. Anseth C. N. Bowman 2006 Controlling network structure in degradable thiol-acrylate biomaterials to tune mass loss behavior Biomacromolecules 7 2827 2836
30. C. N. Salinas K. S. Anseth 2008 Mixed mode thiol-acrylate photopolymerizations for the synthesis of PEG-peptide hydrogels Macromolecules 41 6019 6026
31. C. N. Salinas K. S. Anseth 2008 The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities Biomaterials 29 2370 2377
32. N. B. Cramer C. N. Bowman 2001 Kinetics of thiol-ene and thiol-acrylate photopolymerizations with real-time Fourier transform infrared J. Polym. Sci. A, Polym. Chem. 39 3311 3319
33. N. B. Cramer J. P. Scott C. N. Bowman 2002 Photopolymerizations of thiol-ene polymers without photoinitiators Macromolecules 35 5361 5365
34. N. B. Cramer T. Davies A. K. O'Brien C. N. Bowman 2003 Mechanism and modeling of a thiol-ene photopolymerization Macromolecules 36 4631 4636
35. N. B. Cramer S. K. Reddy M. Cole C. Hoyle C. N. Bowman 2004 Initiation and kinetics of thiol-ene photopolymerizations without photoinitiators J. Polym. Sci. A, Polym. Chem. 42 5817 5826
36. M. D. Scott K. L. Murad 1998 Cellular camouflage: fooling the immune system with polymers Curr. Pharm. Des. 4 423 438
37. G. Csucs R. Michel J. W. Lussi M. Textor G. Danuser 2003 Microcontact printing of novel co-polymers in combination with proteins for cell-biological applications Biomaterials 24 1713 1720
38. L. M. Weber K. N. Hayda K. Haskins K. S. Anseth 2007 The effects of cell-matrix interactions on encapsulated beta-cell function within hydrogels functionalized with matrix-derived adhesive peptides Biomaterials 28 3004 3011
39. N. Gurav M. P. Lutolf G. P. Raeber J. A. Hubbell L. Di Silvio 2007 Differentiation of human bone marrow stem cells within RGD functionalised, proteolytically sensitive PEG gels Tissue Eng. 13 1675 1675
40. C. N. Salinas B. B. Cole A. M. Kasko K. S. Anseth 2007 Chondrogenic differentiation potential of human mesenchymal stem cells photoencapsulated within poly(ethylene glycol)-arginine-glycine-aspartic acid-serine thiol-methacrylate mixed-mode networks Tissue Eng. 13 1025 1034
41. G. D. Nicodemus S. J. Bryant 2008 Cell encapsulation in biodegradable hydrogels for tissue engineering applications Tissue Eng. Part B-Reviews 14 149 165
42. A. T. Metters K. S. Anseth C. N. Bowman 2000 Fundamental studies of a novel, biodegradable PEG-b-PLA hydrogel Polymer 41 3993 4004
43. M. N. Mason A. T. Metters C. N. Bowman K. S. Anseth 2001 Predicting controlled-release behavior of degradable PLA-b-PEG-b-PLA hydrogels Macromolecules 34 4630 4635
44. K. S. Anseth A. T. Metters S. J. Bryant P. J. Martens J. H. Elisseeff C. N. Bowman 2002 In situ forming degradable networks and their application in tissue engineering and drug delivery J. Control. Release 78 199 209
45. M. P. Lutolf J. L. Lauer-Fields H. G. Schmoekel A. T. Metters F. E. Weber G. B. Fields J. A. Hubbell 2003 Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cell-invasion characteristics Proc. Natl. Acad. Sci. U. S. A. 100 5413 5418
46. M. Ehrbar A. Metters P. Zammaretti J. A. Hubbell A. H. Zisch 2005 Endothelial cell proliferation and progenitor maturation by fibrin-bound VEGF variants with differential susceptibilities to local cellular activity J. Control. Release 101 93 109
47. M. A. Rice J. Sanchez-Adams K. S. Anseth 2006 Exogenously triggered, enzymatic degradation of photopolymerized hydrogels with polycaprolactone subunits: experimental observation and modeling of mass loss behavior Biomacromolecules 7 1968 1975
48. M. A. Rice K. S. Anseth 2007 Controlling cartilaginous matrix evolution in hydrogels with degradation triggered by exogenous addition of an enzyme Tissue Eng. 13 683 691
49. N. A. Peppas B. Kim 2006 Stimuli-sensitive protein delivery systems Journal of Drug Delivery Science and Technology 16 11 18
50. M. E. H. El-Sayed A. S. Hoffman P. S. Stayton 2005 Smart polymeric carriers for enhanced intracellular delivery of therapeutic macromolecules Expert Opin. Biol. Ther. 5 23 32
51. A. S. Hoffman P. S. Stayton 2007 Conjugates of stimuli-responsive polymers and proteins Prog. Polym. Sci. 32 922 932
52. J. W. DuBose C. Cutshall A. T. Metters 2005 Controlled release of tethered molecules via engineered hydrogel degradation: model development and validation J. Biomed. Mater. Res. Part A 74A 104 116
53. D. S. W. Benoit S. D. Collins K. S. Anseth 2007 Multifunctional hydrogels that promote osteogenic human mesenchymal stem cell differentiation through stimulation and sequestering of bone morphogenic protein 2 Adv. Funct. Mater. 17 2085 2093
54. A. H. Zisch M. P. Lutolfv M. Ehrbar G. P. Raeber S. C. Rizzi N. Davies H. Schmokel D. Bezuidenhout V. Djonov P. Zilla J. A. Hubbell 2003 Cell-demanded release of VEGF from synthetic, biointeractive cell-ingrowth matrices for vascularized tissue growth FASEB J. 17 2260 2262
55. M. Ehrbar V. G. Djonov C. Schnell S. A. Tschanz G. Martiny-Baron U. Schenk J. Wood P. H. Burri J. A. Hubbell A. H. Zisch 2004 Cell-demanded liberation of VEGF(121) from fibrin implants induces local and controlled blood vessel growth Circ. Res. 94 1124 1132
56. R. G. Schoenmakers P. van de Wetering D. L. Elbert J. A. Hubbell 2004 The effect of the linker on the hydrolysis rate of drug-linked ester bonds J. Control. Release 95 291 300
57. H. Soyez E. Schacht M. Jelinkova B. Rihova 1997 Biological evaluation of mitomycin C bound to a biodegradable polymeric carrier J. Control. Release 47 71 80
58. I. Watermann J. Gerspach M. Lehne J. Seufert B. Schneider K. Pfizenmaier H. Wajant 2007 Activation of CD95L fusion protein prodrugs by tumor-associated proteases Cell Death and Differ. 14 765 774
59. S. E. Sakiyama-Elbert J. A. Hubbell 2000 Controlled release of nerve growth factor from a heparin-containing fibrin-based cell ingrowth matrix J. Control. Release 69 149 158
60. S. E. Sakiyama-Elbert J. A. Hubbell 2000 Development of fibrin derivatives for controlled release of heparin-binding growth factors J. Control. Release 65 389 402
61. C. C. Lin A. T. Metters 2007 Metal-chelating affinity hydrogels for sustained protein release J. Biomed. Mater. Res. Part A 83A 954 964
62. J. Li X. Li X. P. Ni X. Wang H. Z. Li K. W. Leong 2006 Self-assembled supramolecular hydrogels formed by biodegradable PEO-PHB-PEO triblock copolymers and alpha-cyclodextrin for controlled drug delivery Biomaterials 27 4132 4140
63. J. Li X. P. Ni K. W. Leong 2003 Injectable drug-delivery systems based on supramolecular hydrogels formed by poly(ethylene oxide) and alpha-cyclodextrin J. Biomed. Mater. Res. Part A 65A 196 202
64. S. S. Cai Y. C. Liu X. Z. Shu G. D. Prestwich 2005 Injectable glycosaminoglycan hydrogels for controlled release of human basic fibroblast growth factor Biomaterials 26 6054 6067
65. D. B. Pike S. S. Cai K. R. Pomraning M. A. Firpo R. J. Fisher X. Z. Shu G. D. Prestwich R. A. Peattie 2006 Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF Biomaterials 27 5242 5251
66. H. D. Maynard J. A. Hubbell 2005 Discovery of a sulfated tetrapeptide that binds to vascular endothelial growth factor Acta Biomaterialia 1 451 459
67. S. M. Willerth P. J. Johnson D. J. Maxwell S. R. Parsons M. E. Doukas S. E. Sakiyama-Elbert 2007 Rationally designed peptides for controlled release of nerve growth factor from fibrin matrices J. Biomed. Mater. Res. Part A 80A 13 23
68. J. B. Rothbard M. L. Gefter 1991 Interactions between immunogenic peptides and Mhc proteins Annu. Rev. Immunol. 9 527 565
69. J. C. Kang S. P. Schwendeman 2002 Comparison of the effects of Mg(OH)(2) and sucrose on the stability of bovine serum albumin encapsulated in injectable poly(D,L-lactide-co-glycolide) implants Biomaterials 23 239 245
70. G. Z. Zhu S. R. Mallery S. P. Schwendeman 2000 Stabilization of proteins encapsulated in injectable poly (lactide-co-glycolide) Nat. Biotechnol. 18 52 57
71. G. Z. Zhu S. P. Schwendeman 2000 Stabilization of proteins encapsulated in cylindrical poly(lactide-co-glycolide) implants: mechanism of stabilization by basic additives Pharm. Res. 17 351 357
72. D. J. Quick K. S. Anseth 2003 Gene delivery in tissue engineering: a photopolymer platform to coencapsulate cells and plasmid DNA Pharm. Res. 20 1730 1737
73. D. J. Quick K. S. Anseth 2004 DNA delivery from photocrosslinked PEG hydrogels: encapsulation efficiency, release profiles, and DNA quality J. Control. Release 96 341 351
74. C. C. Lin A. T. Metters 2006 Enhanced protein delivery from photopolymerized hydrogels using a pseudospecific metal chelating ligand Pharm. Res. 23 614 622
75. C. C. Lin S. M. Sawicki A. T. Metters 2008 Free-radical-mediated protein inactivation and recovery during protein photoencapsulation Biomacromolecules 9 75 83
76. F. Gu R. Neufeld B. Amsden 2007 Maintenance of vascular endothelial growth factor and potentially other therapeutic proteins bioactivity during a photo-initiated free radical cross-linking reaction forming biodegradable elastomers Eur. J. Pharm. Biopharm. 66 21 27
77. M. Torres-Lugo N. A. Peppas 2002 Preparation and characterization of P(MAA-g-EG) nanospheres for protein delivery applications J Nanopart. Res. 4 73 81
78. B. Kim N. A. Peppas 2003 In vitro release behavior and stability of insulin in complexation hydrogels as oral drug delivery carriers Int. J. Pharm. 266 29 37
79. B. Kim K. La Flamme N. A. Peppas 2003 Dynamic swelling Behavior of pH-sensitive anionic hydrogels used for protein delivery J. Appl. Polym. Sci. 89 1606 1613
80. K. Nakamura R. J. Murray J. I. Joseph N. A. Peppas M. Morishita A. M. Lowman 2004 Oral insulin delivery using P(MAA-g-EG) hydrogels: effects of network morphology on insulin delivery characteristics J. Control. Release 95 589 599
81. L. Serra J. Domenech N. A. Peppas 2006 Drug transport mechanisms and release kinetics from molecularly designed poly(acrylic acid-g-ethylene glycol) hydrogels Biomaterials 27 5440 5451
82. T. Yamagata M. Morishita N. J. Kavimandan K. Nakamura Y. Fukuoka K. Takayama N. A. Peppas 2006 Characterization of insulin protection properties of complexation hydrogels in gastric and intestinal enzyme fluids J. Control. Release 112 343 349
83. L. Serra J. Domenech N. A. Peppas 2006 Design of poly(ethylene glycol)-tethered copolymers as novel mucoadhesive drug delivery systems Eur. J. Pharm. Biopharm. 63 11 18
84. M. Morishita T. Goto K. Nakamura A. M. Lowman K. Takayama N. A. Peppas 2006 Novel oral insulin delivery systems based on complexation polymer hydrogels: Single and multiple administration studies in type 1 and 2 diabetic rats J. Control. Release 110 587 594
85. G. M. McGeehan D. M. Bickett M. Green D. Kassel J. S. Wiseman J. Berman 1994 Characterization of the peptide substrate specificities of interstitial collagenase and 92-kDa gelatinase-implications for substrate optimization J. Biol. Chem. 269 32814 32820
86. T. A. Holland J. K. V. Tessmar Y. Tabata A. G. Mikos 2004 Transforming growth factor-beta 1 release from oligo(poly(ethylene glycol) fumarate) hydrogels in conditions that model the cartilage wound healing environment J. Control. Release 94 101 114
87. T. A. Holland Y. Tabata A. G. Mikos 2005 Dual growth factor delivery from degradable oligo(poly(ethylene glycol) fumarate) hydrogel scaffolds for cartilage tissue engineering J. Control. Release 101 111 125
88. K. Y. Lee M. C. Peters K. W. Anderson D. J. Mooney 2000 Controlled growth factor release from synthetic extracellular matrices Nature 408 998 1000
89. J. Y. Lee S. H. Nam S. Y. Im Y. J. Park Y. M. Lee Y. J. Seol C. P. Chung S. J. Lee 2002 Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials J. Control. Release 78 187 197
90. T. P. Richardson M. C. Peters A. B. Ennett D. J. Mooney 2001 Polymeric system for dual growth factor delivery Nat. Biotechnol. 19 1029 1034
91. D. S. W. Benoit A. R. Durney K. S. Anseth 2007 The effect of heparin-functionalized PEG hydrogels on three-dimensional human mesenchymal stem cell osteogenic differentiation Biomaterials 28 66 77
92. E. K. Moioli L. Hong J. Guardado P. A. Clark J. J. Mao 2006 Sustained release of TGF beta 3 from PLGA microspheres and its effect on early osteogenic differentiation of human mesenchymal stem cells Tissue Eng. 12 537 546
93. J. Sohier D. Hamann M. Koenders N. Cucchiarini H. Madry C. van Blitterswijk K. de Groot J. M. Bezemer 2007 Tailored release of TGF-beta(1) from porous scaffolds for cartilage tissue engineering Int. J. Pharm. 332 80 89
94. A. J. Ridley M. A. Schwartz K. Burridge R. A. Firtel M. H. Ginsberg G. Borisy J. T. Parsons A. R. Horwitz 2003 Cell migration: integrating signals from front to back Science 302 1704 1709
95. T. J. Stefonek K. S. Masters 2007 Immobilized gradients of epidermal growth factor promote accelerated and directed keratinocyte migration Wound Repair Regen. 15 847 855
96. M. Arnold V. C. Hirschfeld-Warneken T. Lohmuller P. Heil J. Blummel E. A. Cavalcanti-Adam M. Lopez-Garcia P. Walther H. Kessler B. Geiger J. P. Spatz 2008 Induction of cell polarization and migration by a gradient of nanoscale variations in adhesive ligand spacing Nano Lett. 8 2063 2069
97. B. M. Lamb D. G. Barrett N. P. Westeott M. N. Yousaf 2008 Microfluidic lithography of SAMs on gold to create dynamic surfaces for directed cell migration and contiguous cell cocultures Langmuir 24 8885 8889
98. Y. Luo M. S. Shoichet 2004 A photolabile hydrogel for guided three-dimensional cell growth and migration Nature Materials 3 249 253
99. M. S. Hahn J. S. Miller J. L. West 2006 Three-dimensional biochemical and biomechanical patterning of hydrogels for guiding cell behavior Adv. Mater. 18 2679 2684
100. M. S. Hahn L. J. Taite J. J. Moon M. C. Rowland K. A. Ruffino J. L. West 2006 Photolithographic patterning of polyethylene glycol hydrogels Biomaterials 27 2519 2524
101. S. H. Lee J. J. Moon J. L. West 2008 Three-dimensional micropatterning of bioactive hydrogels via two-photon laser scanning photolithography for guided 3D cell migration Biomaterials 29 2962 2968
102. J. T. Connelly A. J. Garcia M. E. Levenston 2007 Inhibition of in vitro chondrogenesis in RGD-modified three-dimensional alginate gels Biomaterials 28 1071 1083
103. N. S. Hwang S. Varghese Z. Zhang J. Elisseeff 2006 Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate modified hydrogels Tissue Eng. 12 2695 2706
104. C. Wang R. J. Stewart J. Kopecek 1999 Hybrid hydrogels assembled from synthetic polymers and coiled-coil protein domains Nature 397 417 420
105. C. Wang J. Kopecek R. J. Stewart 2001 Hybrid hydrogels cross-linked by genetically engineered coiled-coil block proteins Biomacromolecules 2 912 920
106. J. D. Ehrick S. K. Deo T. W. Browning L. G. Bachas M. J. Madou S. Daunert 2005 Genetically engineered protein in hydrogels tailors stimuli-responsive characteristics Nature Materials 4 298 302
107. J. D. Ehrick S. Stokes S. Bachas-Daunert E. A. Moschou S. K. Deo L. G. Bachas S. Daunert 2006 Chemically tunable lensing of stimuli-responsive hydrogel microdomes Adv. Mater. 19 4024 4027
108. W. L. Murphy W. S. Dillmore J. Modica M. Mrksich 2007 Dynamic hydrogels: translating a protein conformational change into macroscopic motion Angew. Chem. Int. Ed. 46 3066 3069
109. Z. J. Sui W. J. King W. L. Murphy 2008 Protein-based hydrogels with tunable dynamic responses Adv. Funct. Mater. 18 1824 1831
110. N. Yamaguchi L. Zhang B. S. Chae C. S. Palla E. M. Furst K. L. Kiick 2007 Growth factor mediated assembly of cell receptor-responsive hydrogels J. Am. Chem. Soc. 129 3040 3041
111. S. H. Kim K. L. Kiick 2007 Heparin-mimetic sulfated peptides with modulated affinities for heparin-binding peptides and growth factors Peptides 28 2125 2136
112. T. Nie A. Baldwin N. Yamaguchi K. L. Kiick 2007 Production of heparin-functionalized hydrogels for the development of responsive and controlled growth factor delivery systems J. Control. Release 122 287 296
113. D. S. W. Benoit M. P. Schwartz A. R. Durney K. S. Anseth 2008 Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells Nature Materials 7 816 823
114. D. S. W. Benoit C. R. Nuttelman S. D. Collins K. S. Anseth 2006 Synthesis and characterization of a fluvastatin-releasing hydrogel delivery system to modulate hMSC differentiation and function for bone regeneration Biomaterials 27 6102 6110
115. C. R. Nuttelman D. S. W. Benoit M. C. Tripodi K. S. Anseth 2006 The effect of ethylene glycol methacrylate phosphate in PEG hydrogels on mineralization and viability of encapsulated hMSCs Biomaterials 27 1377 1386
116. C. R. Nuttelman M. C. Tripodi K. S. Anseth 2006 Dexamethasone- functionalized gels induce osteogenic differentiation of encapsulated hMSCs J. Biomed. Mater. Res. Part A 76A 183 195
117. C. N. Salinas, and K. S. Anseth. Decorin moieties tethered into PEG networks induce chondrogenesis of human mesenchymal stem cells. J Biomed. Mater. Res. Part A in press: (2008). doi: 10.1002/jbm.a.32112.
118. C. N. Salinas K. S. Anseth 2008 The influence of the RGD peptide motif and its contextual presentation in PEG gels on human mesenchymal stem cell viability Journal of Tissue Engineering and Regenerative Medicine 2 296 304
119. S. Varghese N. S. Hwang A. C. Canver P. Theprungsirikul D. W. Lin J. Elisseeff 2008 Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells Matrix Biology 27 12 21
120. B. Sharma C. G. Williams M. Khan P. Manson J. H. Elisseeff 2007 In vivo chondrogenesis of mesenchymal stem cells in a photopolymerized hydrogel Plast. Reconstr. Surg. 119 112 120
121. N. S. Hwang M. S. Kim S. Sampattavanich J. H. Baek Z. J. Zhang J. Elisseeff 2006 Effects of three-dimensional culture and growth factors on the chondrogenic differentiation of murine embryonic stem cells Stem Cells 24 284 291
122. D. A. Wang C. G. Williams F. Yang N. Cher H. Lee J. H. Elisseeff 2005 Bioresponsive phosphoester hydrogels for bone tissue engineering Tissue Eng. 11 201 213
123. K. Hemmrich D. von Heimburg 2006 Biomaterials for adipose tissue engineering Expert Rev. Med. Dev. 3 635 645
124. A. V. Vashi E. Keramidaris K. M. Abberton W. A. Morrison J. L. Wilson A. J. O'Connor J. J. Cooper-White E. W. Thompson 2008 Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro Biomaterials 29 573 579