Serum-free culture of primary human hepatocytes in a miniaturized hollow-fibre membrane bioreactor for pharmacological in vitro studies

Journal of Tissue Engineering and Regenerative Medicine

Volumn 9, Issue 9, 2015, Pages 1017-1026

  Lübberstedt, Marc ^a   Müller Vieira, Ursula ^b   Biemel, Klaus M ^b   Darnell, Malin ^c   Hoffmann, Stefan A ^a   Knöspel, Fanny ^a   Wönne, Eva C ^a   Knobeloch, Daniel ^d   Nüssler, Andreas K ^e   Gerlach, Jörg C ^f   Andersson, Tommy B ^c   Zeilinger, Katrin ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Pharmacelsus GmbH   (Germany)

^c ASTRAZENECA R AND D   (Sweden)

^d Cytonet GmbH and Co KG   (Germany)

^e UNIVERSITY OF TÜBINGEN   (Germany)

^f UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Bioreactor; Cytochrome P450; Human hepatocytes; Perfusion; Pharmacological studies; Serum free; Three dimensional culture

Indexed keywords

BIOREACTORS; BODY FLUIDS; ENZYMES; GENE EXPRESSION; METABOLISM; UREA;

BIOREACTOR; CYTOCHROMES P450; FOETAL CALF SERUMS; HUMAN HEPATOCYTES; IN-VITRO; PERFUSION; PHARMACOLOGICAL STUDY; PRIMARY HUMAN HEPATOCYTES; SERUM FREE; THREE-DIMENSIONAL CULTURE;

BIOCONVERSION;

ALANINE AMINOTRANSFERASE; ALBUMIN; ASPARTATE AMINOTRANSFERASE; CYTOCHROME P450 1A2; CYTOCHROME P450 2B6; CYTOCHROME P450 2C9; CYTOCHROME P450 2D6; CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; GLUCOSE; GLUTAMATE DEHYDROGENASE; LACTIC ACID; UREA; ARTIFICIAL MEMBRANE; CYTOCHROME P450; SERUM-FREE MEDIUM;

ANAEROBIC GLYCOLYSIS; ARTICLE; CELL METABOLISM; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME RELEASE; ENZYME SPECIFICITY; ENZYME STABILITY; GENE EXPRESSION; GLUCOSE METABOLISM; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL CULTURE; MEMBRANE REACTOR; PRIORITY JOURNAL; PROTEIN SYNTHESIS; UREA CYCLE; ARTIFICIAL MEMBRANE; BIOREACTOR; CELL CULTURE; CYTOLOGY; ENZYMOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION PROFILING; LIVER CELL; METABOLISM; MINIATURIZATION; PRECLINICAL STUDY; SERUM-FREE MEDIUM;

BIOREACTORS; CELLS, CULTURED; CULTURE MEDIA, SERUM-FREE; CYTOCHROME P-450 ENZYME SYSTEM; DRUG EVALUATION, PRECLINICAL; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION PROFILING; HEPATOCYTES; HUMANS; IN VITRO TECHNIQUES; MEMBRANES, ARTIFICIAL; MINIATURIZATION;

EID: 84941599468     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.1652     Document Type: Article

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