Hepatic 3D cultures but not 2D cultures preserve specific transporter activity for acetaminophen-induced hepatotoxicity

Archives of Toxicology

Volumn 87, Issue 8, 2013, Pages 1581-1593

  Schyschka, L ^a   Sánchez, J J Martínez ^b   Wang, Z ^a   Burkhardt, B ^b   Muller Vieira U ^c   Zeilinger, K ^d   Bachmann, A ^b   Nadalin, S ^a   Damm, G ^d   Nussler, A K ^b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c Pharmacelsus GmbH   (Germany)

^d CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

2D culture; 3D culture; Acetaminophen; Drug metabolism; Primary human hepatocytes

Indexed keywords

ALANINE AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; CYTOCHROME P450 2E1; LACTATE DEHYDROGENASE; MULTIDRUG RESISTANCE PROTEIN 1; PARACETAMOL; SUPEROXIDE DISMUTASE;

ARTICLE; CELL CULTURE; CELL DEATH; CELL DIFFERENTIATION; CONTROLLED STUDY; CYTOTOXICITY TEST; DRUG METABOLISM; ENZYME ACTIVITY; ENZYME RELEASE; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL; LIVER TOXICITY; MRP1 GENE; PRIORITY JOURNAL; PROTEIN EXPRESSION; THREE DIMENSIONAL CULTURE; TWO DIMENSIONAL CULTURE;

ACETAMINOPHEN; CELL CULTURE TECHNIQUES; CELL DEATH; CYTOCHROME P-450 CYP2E1; DOSE-RESPONSE RELATIONSHIP, DRUG; HEPATOCYTES; HUMANS; LIVER; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; PRIMARY CELL CULTURE; SUPEROXIDE DISMUTASE;

EID: 84883452901     PISSN: 03405761     EISSN: 14320738     Source Type: Journal    
DOI: 10.1007/s00204-013-1080-y     Document Type: Article

References (38)

1. Agarwal R, MacMillan-Crow LA et al (2011) Acetaminophen-induced hepatotoxicity in mice occurs with inhibition of activity and nitration of mitochondrial manganese superoxide dismutase. J Pharmacol Exp Ther 337(1):110-116
2. Aleksunes LM, Augustine LM et al (2007) Influence of acetaminophen vehicle on regulation of transporter gene expression during hepatotoxicity. J Toxicol Environ Health A 70(21):1870-1872
3. Arndt K, Haschek WM et al (1989) Mechanism of dimethylsufoxide protection against acetaminophen hepatotoxicity. Drug Metab Rev 20:261-269
4. Bruschi SA (2004) Methods and approaches to study metabolism and toxicity of acetaminophen. In: Drug metabolism and transport. Totowa, NJ, pp 197-232
5. Cole SP, Deeley RG (2006) Transport of gluthathione and gluthathione conjugates by MRP1. Trends Pharmacol Sci 27(8):438-446
6. Decaens C, Durand M et al (2008) Which in vitro models could be best used to study hepatocyte polarity? Biol Cell 100(7):387-398
7. Eckl PM, Bresgen N (2003) The cultured primary hepatocyte and its application in toxicology. J Appl Biomed 1:117-126
8. Espinosa Bosch M, Ruiz Sanchez AJ et al (2006) Determination of paracetamol: historical evolution. J Pharm Biomed Anal 42(3):291-321
9. Fontana RJ (2008) Acute liver failure including acetaminophen overdose. Med Clin North Am 92(4):761-794, viii
10. Forster S, Thumser AE et al (2012) Characterization of rhodamine-123 as a tracer dye for use in in vitro drug transport assays. PLoS One 7(3):e33253
11. Glavinas H, Krajcsi P et al (2004) The role of ABC transporters in drug resistance, metabolism and toxicity. Curr Drug Deliv 1(1):27-42
12. Godoy P, Hengstler JG et al (2009) Extracellular matrix modulates sensitivity of hepatocytes to fibroblastoid dedifferentiation and transforming growth factor beta-induced apoptosis. Hepatology 49(6):2031-2043
13. Gomez-Lechon MJ, Castell JV et al (2008) An update on metabolism studies using human hepatocytes in primary culture. Expert Opin Drug Metab Toxicol 4(7):837-854
14. Gonzalez FJ (2005) Role of cytochromes P450 in chemical toxicity and oxidative stress: studies with CYP2E1. Mutat Res 569(1-2):101-110
15. Hewitt NJ, Lechon MJ et al (2007) Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies. Drug Metab Rev 39(1):159-234
16. Hinson JA, Roberts DW, et al. (2010) Mechanisms of acetaminophen-induced liver necrosis. Handb Exp Pharmacol(196):369-405
17. Hirano M, Maeda K et al (2005) Bile salt export pump (BSEP/ABCB11) can transport a nonbile acid substrate, pravastatin. J Pharmacol Exp Ther 314:876-882
18. Jemmitz K, Veres Z et al (2010) Contribution of high basolateral bile salt efflux to the lack of hepatotoxicity in rat in response to drugs inducing cholestasis in human. Toxicol Sci 115(1):80-88
19. Kock K, Brouwer KL (2012) A perspective on efflux transport proteins in the liver. Clin Pharmacol Ther 92(5):599-612
20. Kullak-Ublick GA, Stieger B et al (2000) Hepatic transport of bile salts. Semin Liver Dis 20(3):273-292
21. Larson AM, Polson J et al (2005) Acetaminophen-induced acute liver failure: results of a United States multicenter, prospective study. Hepatology 42(6):1364-1372
22. Laupeze B, Amiot L et al (2001) Multidrug resistance protein (MRP) activity in normal mature leukocytes and CD34-positive hematopoietic cells from peripheral blood. Life Sci 68(11):1323-1331
23. Leslie EM, Deeley RG et al (2001) Toxicological relevance of the multidrug resistance protein 1, MRP1 (ABCC1) and related transporters. Toxicology 167(1):3-23
24. Li FC, Liu Y et al (2009) In vivo dynamic metabolic imaging of obstructive cholestasis in mice. Am J Physiol Gastrointest Liver Physiol 296(5):G1091-G1097
25. Li J, Wei DQ et al (2012) Molecular dynamics simulations of CYP2E1. Med Chem 8(2):208-221
26. Lin J, Schyschka L et al (2012) Comparative analysis of phase I and II enzyme activities in 5 hepatic cell lines identifies Huh-7 and HCC-T cells with the highest potential to study drug metabolism. Arch Toxicol 86(1):87-95
27. Lowry OH, Rosebrough NJ et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265-275
28. Madsen KG, Olsen J et al (2007) Development and evaluation of an electrochemical method for studying reactive phase-I metabolites: correlation to in vitro drug metabolism. Chem Res Toxicol 20(5):821-831
29. Nussler AK, Nussler NC et al (2009) The Holy grail of hepatocyte culturing and therapeutic use. In: Santin M (ed) Strategies in regenerative medicine. Springer International, Germany
30. Prot JM, Briffaut AS et al (2011) Integrated proteomic and transcriptomic investigation of the acetaminophen toxicity in liver microfluidic biochip. PLoS One 6(8):e21268
31. Ramachandran A, Lebofsky M et al (2011) The impact of partial manganese superoxide dismutase (SOD2)-deficiency on mitochondrial oxidant stress, DNA fragmentation and liver injury during acetaminophen hepatotoxicity. Toxicol Appl Pharmacol 251(3):226-233
32. Roelofsen H, Hooiveld GJ et al (1999) Glutathione S-conjugate transport in hepatocytes entering the cell cycle is preserved by a switch in expression from the apical MRP2 to the basolateral MRP1 transporting protein. J Cell Sci 112(Pt 9):1395-1404
33. Rost D, Kartenbeck J et al (1999) Changes in the localization of the rat canalicular conjugate export pump Mrp2 in phalloidin-induced cholestasis. Hepatology 29(3):814-821
34. Schutte M, Fox B et al (2011) Rat primary hepatocytes show enhanced performance and sensitivity to acetaminophen during three-dimensional culture on a polystyrene scaffold designed for routine use. Assay Drug Dev Technol 9(5):475-486
35. Suzuki N, Higuchi T et al (2002) Multiple active intermediates in oxidation reaction catalyzed by synthetic heme-thiolate complex relevant to cytochrome p450. J Am Chem Soc 124(32):9622-9628
36. Ullrich A, Berg C et al (2006) Use of a novel; standardized and validated human hepatocyte culture system for repetitive analyses of drugs: repeated administrations of acetaminophen reduces albumin and urea secretion. Faseb Journal 20(4):A630-A630
37. Yamaura K, Shimada M et al (2011) Protective effects of goldenseal (Hydrastis canadensis L.) on acetaminophen-induced hepatotoxicity through inhibition of CYP2E1 in rats. Pharmacognosy Res 3(4):250-255
38. Zaher H, Buters JT et al (1998) Protection against acetaminophen toxicity in CYP1A2 and CYP2E1 double-null mice. Toxicol Appl Pharmacol 152(1):193-199