Human hepatocytes: Isolation, culture, and quality procedures

Methods in Molecular Biology

Volumn 806, Issue , 2012, Pages 99-120

  Knobeloch, Daniel ^a   Ehnert, Sabrina ^b   Schyschka, Lilianna ^c   Büchler, Peter ^c   Schoenberg, Michael ^d   Kleeff, Jörg ^c   Thasler, Wolfgang E ^e   Nussler, Natascha C ^f   Godoy, Patricio ^g   Hengstler, Jan ^g   Nussler, Andreas K ^b,c  

^a Cytonet Gmbh   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^d UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^e UNIVERSITY OF MUNICH   (Germany)

^f Klinikum Neuperlach   (Germany)

^g LEIBNIZ RESEARCH CENTRE FOR WORKING ENVIRONMENT AND HUMAN FACTORS   (Germany)

Author keywords

Cell isolation; Hepatocyte culture; Human hepatocytes; Metabolic competence; Quality control

Indexed keywords

ARTICLE; CELL CULTURE; CELL SEPARATION; CYTOLOGY; ENZYMOLOGY; HUMAN; LIVER CELL; METABOLISM; METHODOLOGY; PRIMARY CELL CULTURE; QUALITY CONTROL; STANDARD;

CELL SEPARATION; CELLS, CULTURED; HEPATOCYTES; HUMANS; PRIMARY CELL CULTURE; QUALITY CONTROL;

EID: 82355193824     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-367-7_8     Document Type: Article

References (21)

1. Branster, M. V., and Morton, R. K. (1957) Isolation of intact liver cells. Nature. 180, 1283-1284.
2. de Boer, J., and van Bavel, B. (2009) European "REACH" (Registration, Evaluation, Authorisation and Restriction of Chemicals) program. J Chromatogr A. 1216, 301.
3. Alexandre, E., Cahn, M., Abadie-Viollon, C., Meyer, N., Heyd, B., Mantion, G., et al. (2002) Influence of pre-, intra- and post-operative parameters of donor liver on the outcome of isolated human hepatocytes. Cell Tissue Bank. 3, 223 - 233. (Pubitemid 36950118)
4. Lloyd, T. D., Orr, S., Patel, R., Crees, G., Chavda, S., Vadyar, H., et al. (2004) Effect of patient, operative and isolation factors on subsequent yield and viability of human hepatocytes for research use. Cell Tissue Bank. 5, 81 - 87. (Pubitemid 38961751)
5. Seglen, P. O. (1976) Preparation of isolated rat liver cells. Methods Cell Biol. 13, 29 - 83.
6. Dorko, K., Freeswick, P. D., Bartoli, F., Cicalese, L., Bardsley, B. A., Tzakis, A., et al. (1994) A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell Transplant. 3, 387 - 395. (Pubitemid 24286981)
7. Sharma, A. D., Cantz, T., Richter, R., Eckert, K., Henschler, R., Wilkens, L., et al. (2005) Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver. Am J Pathol. 167, 555 - 564. (Pubitemid 43026109)
8. Beerheide, W., von Mach, M. A., Ringel, M., Fleckenstein, C., Schumann, S., Renzing, N., et al. (2002) Downregulation of beta2- microglobulin in human cord blood somatic stem cells after transplantation into livers of SCID-mice: an escape mechanism of stem cells? Biochem Biophys Res Commun. 294, 1052-1063. (Pubitemid 34687157)
9. Hengstler, J. G., Brulport, M., Schormann, W., Bauer, A., Hermes, M., Nussler, A. K., et al. (2005) Generation of human hepatocytes by stem cell technology: definition of the hepatocyte. Expert Opin Drug Metab Toxicol. 1, 61 - 74.
10. Williams, R. T. (1959) Detoxification Mechanisms. New York: John Wiley & Sons.
11. Parkinson, A. (1996) Biotransformation of xenobiotics. In Casarett and Doull's Toxicology: the basic Science of Poinsons, ed. Klaassen, C. D. New York: McGraw-Hill. 113-186.
12. Nelson, D. R., Zeldin, D. C., Hoffman, S. M., Maltais, L. J., Wain, H. M., and Nebert, D. W. (2004) Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative- splice variants. Pharmacogenetics. 14, 1 - 18. (Pubitemid 38183957)
13. Guengerich, F. P., and MacDonald, T. L. (1990) Mechanisms of cytochrome P-450 catalysis. FASEB J. 4, 2453 - 2459.
14. Pelkonen, O., Maenpaa, J., Taavitsainen, P., Rautio, A., and Raunio, H. (1998) Inhibition and induction of human cytochrome P450 (CYP) enzymes. Xenobiotica. 28, 1203 - 1253. (Pubitemid 29017141)
15. Donato, M. T., Jimenez, N., Castell, J. V., and Gomez-Lechon, M. J. (2004) Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab Dispos. 32, 699 - 706. (Pubitemid 38821870)
16. Lin, J. H., and Lu, A. Y. (1997) Role of pharmacokinetics and metabolism in drug discovery and development. Pharmacol Rev. 49, 403 - 449. (Pubitemid 28030947)
17. Hewitt, N. J., Lechon, M. J., Houston, J. B., Hallifax, D., Brown, H. S., Maurel, P., et al. (2007) Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies. Drug Metab Rev. 39, 159 - 234.
18. Guillouzo, A., Morel, F., Fardel, O., and Meunier, B. (1993) Use of human hepatocyte cultures for drug metabolism studies. Toxicology. 82, 209 - 219.
19. Rajan, N., Habermehl, J., Cote, M. F., Doillon, C. J., and Mantovani, D. (2006) Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications. Nat Protoc. 1, 2753 - 2758.
20. Decaens, C., Durand, M., Grosse, B., and Cassio, D. (2008) Which in vitro models could be best used to study hepatocyte polarity? Biol Cell. 100, 387 - 398. (Pubitemid 351943944)
21. Rennert, B., and Melzig, M. F. (2002) Free fatty acids inhibit the activity of Clostridium histolyticum collagenase and human neutrophil elastase. Planta Med. 68, 7 67 - 769.