Protein extraction from plant tissues for 2DE and its application in proteomic analysis

Proteomics

Volumn 14, Issue 6, 2014, Pages 645-658

  Wu, Xiaolin ^a   Gong, Fangping ^a   Wang, Wei ^a  

^a HENAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

2DE; Plant proteomics; Sample preparation; Tutorial

Indexed keywords

ACETONE; METHANOL; PHENOL; TRICHLOROACETIC ACID;

ARTICLE; COMPARATIVE STUDY; EXTRACTION; NONHUMAN; PISTACIA; PLANT TISSUE; PRECIPITATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEOMICS;

2DE; PLANT PROTEOMICS; SAMPLE PREPARATION; TUTORIAL;

ACETONE; CHEMICAL PRECIPITATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; METHANOL; PHENOLS; PISTACIA; PLANT PROTEINS; PLANTS; PROTEOME; PROTEOMICS;

EID: 84895750134     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201300239     Document Type: Article

References (120)

1. Weiss, W., Görg, A., Sample solublization buffers for two-dimensional electrophoresis. Methods Mol. Biol. 2008, 424, 35-42.
2. Rabilloud, T., Lelong, C., Two-dimensional gel electrophoresis in proteomics: a tutorial. J. Proteomics 2011, 74, 1829-1841.
3. Cottrell, J. S., Protein identification using MS/MS data. J. Proteomics 2011, 74, 1842-1851.
4. Méchin, V., Damerval, C., Zivy, M., Total protein extraction with TCA-acetone. Methods Mol. Biol. 2007, 355, 1-8.
5. Görg, A, Weiss, W., in: Rabilloud, T. (Ed.), Proteome Research: Two-Dimensional Electrophoresis and Identification Methods. Springer, Berlin, 2000, p. 57-106.
6. Damerval, C., Zivy, M., Granier, F., de Vienne, D., Two-dimensional electrophoresis in plant biology, in: Chrambach, A., Dunn, M., Radola, B. (Eds.), Advances in Electrophoresis. VCH, Weinheim, New York, 1988, pp. 265-340.
7. Shaw, M. M., Riederer, B. M., Sample preparation for two-dimensional gel electrophoresis. Proteomics 2003, 3, 1408-1417.
8. Damerval, C., De Vienne, D., Zivy, M., Thiellement, H., Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat seedling proteins. Electrophoresis 1986, 7, 52-54.
9. Granier, F., Extraction of plant proteins for two-dimensional electrophoresis. Electrophoresis 1988, 9, 712-718.
10. Meyer, Y., Grosser, J., Chartier, Y., Cleyet-Marel, J. C., Preparation by two-dimensional electrophoresis of proteins for antibody production: antibodies against proteins whose synthesis is reduced by auxin in tobacco mesophyll protoplasts. Electrophoresis 1988, 9, 704-712.
11. Rabilloud, T., Solubilization of proteins for electrophoretic analyses. Electrophoresis 1996, 17, 813-829.
12. Wang, W., Scali, M., Vignani, R., Spadafora, A. et al., Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds. Electrophoresis 2003, 24, 2369-2375.
13. Carpentier, S. C., Witters, E., Laukens, K., Deckers, P. et al., Preparation of protein extracts from recalcitrant plant tissues: an evaluation of different methods for two-dimensional gel electrophoresis analysis. Proteomics 2005, 5, 2497-2507.
14. Cho, K., Torres, N. L., Subramanyam, S., Deepak, S. N. et al., Protein extraction/solubilization protocol for monocot and dicot plant gel-based proteomics. J. Plant Biol. 2006, 49, 413-420.
15. Isaacson, T., Damasceno, C. M., Saravanan, R. S., He, Y. et al., Sample extraction techniques for enhanced proteomic analysis of plant tissues. Nat. Protoc. 2006, 1, 769-774.
16. Herbert, B. R., Righetti, P. G., Citterio, A., Boschetti, E., in: Wilkins, M. R., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (Eds.), Proteome Research: Concepts, Technology and Applications, Springer, Berlin, Heidelberg, 2007, pp. 15-40.
17. Wu, X. L., Xiong, E. H., Wang, W., Scali, M., Cresti, M., Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis. Nat. Protoc. 2014, doi:10.1038/nprot.2014.022.
18. O'Farrell, P. H., High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 1975, 250, 4007-4021.
19. Hari, V., A method for the two-dimensional electrophoresis of leaf proteins. Anal. Biochem. 1981, 113, 332-335.
20. Schuster, A., Davies, E., Ribonucleic acid and protein metabolism in pea epicotyls III. Response to auxin in aged tissue. Plant Physiol. 1983, 73, 809-816.
21. Wu, F., Wang, M., Extraction of proteins for sodium dodecyl sulfate polyacrylamide gel electrophoresis from protease-rich plant tissues. Anal. Biochem. 1984, 139, 100-103.
22. Cremer, F., Van de Walle, C., Methods for extraction of proteins from green plant tissues for two-dimensional polyacrylamide gel electrophoresis. Anal. Biochem. 1985, 147, 22-26.
23. Flengsrud, R., Kobro, G., A method for two-dimensional electrophoresis of proteins from green plant tissues. Anal. Biochem. 1989, 177, 33-36.
24. Hurkman, W. J., Tanaka, C. K., Solubilization of plant membrane proteins for analysis by two-dimensional electrophoresis. Plant Physiol. 1986, 81, 802-806.
25. Grassman, W., Deffner, G., Verteilungschromatographisches verhalten von proteinen und peptiden in phenolhaltigen lösungsmitteln. Hoppe-Seylor's Z Physiol. Chem. 1953, 293, 89-98.
26. Görg, A., Weiss, W., Dunn, M. J., Current two-dimensional electrophoresis technology for proteomics. Proteomics 2004, 4, 3665-3685.
27. Rose, J. K., Bashir, S., Giovannoni, J. J., Jahn, M. M., Saravanan, R. S., Tackling the plant proteome: practical approaches, hurdles and experimental tools. Plant J. 2004, 39, 715-733.
28. Bodzon-Kulakowska, A., Bierczynska-Krzysik, A., Dylag, T., Drabik, A. et al., Methods for samples preparation in proteomic research. J. Chromatogr. B 2007, 849, 1-31.
29. Wang, W., Tai, F. J., Chen, S. N., Optimizing protein extraction from plant tissues for enhanced proteomics analysis. J. Sep. Sci. 2008, 31, 2032-2039.
30. Stalikas, C. D., Extraction, separation, and detection methods for phenolic acids and flavonoids. J. Sep. Sci. 2007, 30, 3268-3295.
31. Croteau, R., Kutchan, T. M., Lewis, N. G., in: Buchanan, B., Gruissem, W., Jones, R. (Eds.) Biochemistry and Molecular Biology of Plants, American Society of Plant Physiologists, CRC Press, Rockville, MO, 2000, pp. 1250-1318.
32. Vâlcu, C. M., Schlink, K., Reduction of proteins during sample preparation and two-dimensional gel electrophoresis of woody plant samples. Proteomics 2006a, 6, 1599-1605.
33. Wang, W., Vignani, R., Scali, M., Sensi, E. et al., Removal of lipid contaminants by organic solvents from oilseed protein extract prior to electrophoresis. Anal. Biochem. 2004, 329, 139-141.
34. Toth, G. B., Pavia, H., Removal of dissolved brown algal phlorotannins using insoluble polyvinylpolypyrrolidone (PVPP). J. Chem. Ecol. 2001, 27, 1899-1910.
35. Lerouxel, O., Cavalier, D. M., Liepman, A. H., Keegstra, K., Biosynthesis of plant cell wall polysaccharides-a complex process. Curr. Opin. Plant Biol. 2006, 9, 621-630.
36. Vâlcu, C. M., Schlink, K., Efficient extraction of proteins from woody plant samples for two-dimensional gel electrophoresis. Proteomics 2006b, 6, 4166-4176.
37. Malik, N. S. A., Bradford, J. M., A simple protein extraction method for proteomic studies on olive leaves. J. Food Agric. Environ. 2005, 3, 246-248.
38. Englard, S., Seifter, S., Precipitation techniques. Methods Enzymol. 1990, 182, 285-300.
39. Giavalisco, P., Nordhoff, E., Lehrach, H., Gobom, J., Klose, J., Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis. Electrophoresis 2003, 24, 207-216.
40. Rodrigues, S. P., Ventura, J. A., Zingali, R. B., Fernandes, P. M. B., Evaluation of sample preparation methods for the analysis of papaya leaf proteins through two-dimensional gel electrophoresis. Phytochem. Analysis 2009, 20, 456-464.
41. Nawrocki, A., Thorup-Kristensen, K., Jensen, O. N., Quantitative proteomics by 2DE and MALDI MS/MS uncover the effects of organic and conventional cropping methods on vegetable products. J. Proteomics 2011, 74, 2810-2825.
42. Zadražnik, T., Hollung, K., Egge-Jacobsen, W., Meglič, V., Šuštar-Vozlič, J., Differential proteomic analysis of drought stress response in leaves of common bean (Phaseolus vulgaris L.). J. Proteomics 2013, 78, 254-272.
43. Wang, W., Vignani, R., Scali, M., Cresti, M., A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 2006, 27, 2782-2786.
44. Dogra, V., Ahuja, P. S., Sreenivasulu, Y., Change in protein content during seed germination of a high altitude plant Podophyllum hexandrum Royle. J. Proteomics 2013, 78, 26-38.
45. Balbuena, T. S., Silveira, V., Junqueira, M., Dias, L. L. et al., Changes in the 2-DE protein profile during zygotic embryogenesis in the Brazilian pine (Araucaria angustifolia). J. Proteomics 2009, 72, 337-352.
46. Link, A. J., LaBaer, J., Trichloroacetic acid (TCA) precipitation of proteins. Cold Spring Harb. Protoc. 2011, 2011, 993-994.
47. Meisrimler, C. N., Lüthje, S., IPG-strips versus off-gel fractionation: advantages and limits of two-dimensional PAGE in separation of microsomal fractions of frequently used plant species and tissues. J. Proteomics 2012, 75, 2550-2562.
48. Saravanan, R. S., Rose, J. K., A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues. Proteomics 2004, 4, 2522-2532.
49. Vincent, D., Wheatley, M. D., Cramer, G. R., Optimization of protein extraction and solubilization for mature grape berry clusters. Electrophoresis 2006, 27, 1853-1865.
50. Görg, A., Obermaier, C., Boguth, G., Csordas, A. et al., Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins. Electrophoresis 1997, 18, 328-337.
51. Chen, S., Harmon, A. C., Advances in plant proteomics. Proteomics 2006, 6, 5504-5516.
52. Faurobert, M., Pelpoir, E., Chaïb, J., Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues. Methods Mol. Biol. 2007, 355, 9-14.
53. Marsoni, M., Vannini, C., Campa, M., Cucchi, U. et al., Protein extraction from grape tissues by two-dimensional electrophoresis. Vitis 2005, 44, 181-186.
54. Bona, E., Marsano, F., Massa, N., Cattaneo, C. et al., Proteomic analysis as a tool for investigating arsenic stress in Pteris vittata roots colonized or not by arbuscular mycorrhizal symbiosis. J Proteomics 2011, 74, 1338-1350.
55. Petriccione, M., Di Cecco, I., Arena, S., Scaloni, A., Scortichini, M., Proteomic changes in Actinidia chinensis shoot during systemic infection with a pandemic Pseudomonas syringae pv. Actinidiae strain. J. Proteomics. 2013, 78, 461-476.
56. Dupont, F. M., Tanaka, C. K., Hurkman, W. J., Separation and immunological characterization of membrane fractions from barley roots. Plant Physiol. 1988, 86, 717-724.
57. Song, J., Braun, G., Bevis, E., Doncaster, K., A simple protocol for protein extraction of recalcitrant fruit tissues suitable for 2-DE and MS analysis. Electrophoresis 2006, 27, 3144-3151.
58. Deytieux, C., Geny, L., Lapaillerie, D., Claverol, S. et al., Proteome analysis of grape skins during ripening. J. Exp. Bot. 2007, 58, 1851-1862.
59. Negri, A. S., Prinsi, B., Scienza, A., Morgutti, S. et al., Analysis of grape berry cell wall proteome: a comparative evaluation of extraction methods. J. Plant Physiol. 2008, 165, 1379-1389.
60. Vander Mijnsbrugge, K., Meyermans, H., van Montagu, M., Bauw, G., Boerjan, W., Wood formation in poplar: identification, characterization, and seasonal variation of xylem proteins. Planta 2000, 210, 589-598.
61. Bayer, E. M., Bottrill, A. R., Walshaw, J., Vigouroux, M. et al., Arabidopsis cell wall proteome defined using multidimensional protein identification technology. Proteomics 2006, 6, 301-311.
62. Zheng, Q., Song, J., Doncaster, K., Rowland, E., Byers, D. M., Qualitative and quantitative evaluation of protein extraction protocols for apple and strawberry fruit suitable for two dimensional electrophoresis and mass spectrometry analysis. J. Agric. Food Chem. 2007, 55, 1663-1673.
63. Hu, X. L., Wu, X. L., Li, C. H., Lu, M. H. et al., Abscisic acid refines the synthesis of chloroplast proteins in maize (Zea mays) in response to drought and light. PLoS ONE 2012, 7, e49500.
64. Briante, R., Patumi, M., Terenziani, S., Bismuto, E. et al., Olea europaea, L. leaf extract and derivatives: antioxidant properties. J. Agric. Food Chem. 2002, 50, 4934-4940.
65. Röhrig, H., Schmidt, J., Colby, T., Bräutigam, A. et al., Desiccation of the resurrection plant Craterostigma plantagineum induces dynamic changes in protein phosphorylation. Plant Cell Environ. 2006, 29, 1606-1617.
66. Gómez-Vidal, S., Tena, M., Lopez-Llorca, L. V., Salinas, J., Protein extraction from Phoenix dactylifera L. leaves, a recalcitrant material, for two-dimensional electrophoresis. Electrophoresis 2008, 29, 448-456.
67. Dam, S., Laursen, B. S., Ornfelt, J. H., Jochimsen, B. et al., The proteome of seed development in the model legume Lotus japonicus. Plant Physiol. 2009, 149, 1325-1340.
68. Sghaier-Hammami, B., Drira, N., Jorrín-Novo, J. V., Comparative 2-DE proteomic analysis of date palm (Phoenix dactylifera L.) somatic and zygotic embryos. J. Proteomics 2009, 73, 161-177.
69. Aranjuelo, I., Molero, G., Erice, G., Avice, J. C., Nogués, S., Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.). J. Exp. Bot. 2011, 62, 111-123.
70. Zhao, P. M., Wang, L. L., Han, L. B., Wang, J. et al., Proteomic identification of differentially expressed proteins in the Ligon lintless mutant of upland cotton (Gossypium hirsutum L.). J. Proteome Res. 2010, 9, 1076-1087.
71. Madsen, E. B., Antolín-Llovera, M., Grossmann, C., Ye, J. et al., Autophosphorylation is essential for the in vivo function of the Lotus japonicus Nod factor receptor 1 and receptor-mediated signalling in cooperation with Nod factor receptor 5. Plant J. 2011, 65, 404-417.
72. Maldonado, A. M., Echevarría-Zomeño, S., Jean-Baptiste, S., Hernández, M., Jorrín-Novo, J. V., Evaluation of three different protocols of protein extraction for Arabidopsis thaliana leaf proteome analysis by two-dimensional electrophoresis. J. Proteomics 2008, 71, 461-472.
73. Jorrín, J. V., Maldonado, A. M., Castillejo, M. A., Plant proteome analysis: a 2006 update. Proteomics 2007, 7, 2947-2962.
74. Wang, W., Wu, X. L., Xiong, E. H., Tai, F. J., Improving gel-based proteome analysis of soluble protein extracts by heat prefractionation. Proteomics 2012, 12, 938-943.
75. Wu, X. L., Xiong, E. H., An, S. F., Gong, F. P., Wang, W., Sequential extraction results in improved proteome profiling of medicinal plant pinellia ternata tubers, which contain large amounts of high-abundance proteins. PLoS ONE 2012, 7, e50497.
76. Rabilloud, T., Adessi, C., Giraudel, A., Lunardi, J., Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 1997, 18, 307-316.
77. Galvani, M., Rovatti, L., Hamdan, M., Herbert, B., Righetti, P. G., Protein alkylation in the presence/absence of thiourea in proteome analysis: a matrix assisted laser desorption/ionization-time of flight-mass spectrometry investigation. Electrophoresis 2001, 22, 2066-2074.
78. Castellanos-Serra, L., Paz-Lago, D., Inhibition of unwanted proteolysis during sample preparation: evaluation of its efficiency in challenge experiments. Electrophoresis 2002, 23, 1745-1753.
79. Molloy, M. P., Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients. Anal. Biochem. 2000, 280, 1-10.
80. Herbert, B. R., Molloy, M. P., Gooley, A. A., Walsh, B. J. et al., Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis 1998, 19, 845-851.
81. Méchin, V., Consoli, L., Le Guilloux, M., Damerval, C., An efficient solubilization buffer for plant proteins focused in immobilized pH gradients. Proteomics 2003, 3, 1299-1302.
82. Herbert, B., Advances in protein solubilisation for two-dimensional electrophoresis. Electrophoresis 1999, 20, 660-663.
83. Xiong, E. H., Wu, X. L., Shi, J., Wang, X. Y., Wang, W., Proteomic identification of differentially expressed proteins between male and female plants in Pistacia chinensis. PLoS ONE 2013, 8, e64276.
84. Li, Y. J., Zhang, X. Y., Wang, F. X., Yang, C. L. et al., A comparative proteomic analysis provides insights into pigment biosynthesis in brown colored fiber. J. Proteomics 2013, 78, 374-388.
85. Prassinos, C., Rigas, S., Kizis, D., Vlahou, A., Hatzopoulos, P., Subtle proteome differences identified between post-dormant vegetative and floral peach buds. J. Proteomics 2011, 74, 607-619.
86. Hossain, Z., Makino, T., Komatsu, S., Proteomic study of β-aminobutyric acid-mediated cadmium stress alleviation in soybean. J. Proteomics 2012, 75, 4151-4164.
87. Sheoran, I. S., Ross, A. R., Olson, D. J., Sawhney, V. K., Differential expression of proteins in the wild type and 7B-1 male-sterile mutant anthers of tomato (Solanum lycopersicum): a proteomic analysis. J. Proteomics 2009, 71, 624-636.
88. Zhang, D., Ren, L., Yue, J. H., Wang, L. et al., A comprehensive analysis of flowering transition in Agapanthus praecox ssp. orientalis (Leighton) Leighton by using transcriptomic and proteomic techniques. J. Proteomics 2013, 80, 1-25.
89. D'Ambrosio, C., Arena, S., Rocco, M., Verrillo, F. et al., Proteomic analysis of apricot fruit during ripening. J. Proteomics 2013, 78, 39-57.
90. Vincent, D., Ergül, A., Bohlman, M. C., Tattersall, E. A. R. et al., Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity. J. Exp. Bot. 2007, 58, 1873-1892.
91. Rossignol, M., Analysis of the plant proteome. Curr. Opin. Biotech. 2001, 12, 131-134.
92. Agrawal, G. K., Sarkar, A., Righetti, P. G., Pedreschi, R. et al., A decade of plant proteomics and mass spectrometry: translation of technical advancements to food security and safety issues. Mass Spectrom. Rev. 2013, 32, 335-365.
93. Gao, L. Y., Wang, A. L., Li, X. H., Dong, K. et al., Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE). J. Proteomics 2009, 73, 279-296.
94. Islam, M. A., Sturrock, R. N., Ekramoddoullah, A. K., A proteomics approach to identify proteins differentially expressed in Douglas-fir seedlings infected by Phellinus sulphurascens. J. Proteomics 2008, 71, 425-438.
95. Thomas, L., Marondedze, C., Ederli, L., Pasqualini, S., Gehring, C., Proteomic signatures implicate cAMP in light and temperature responses in Arabidopsis thaliana. J. Proteomics 2013, 83, 47-59
96. Miranda, H., Cheregi, O., Netotea, S., Hvidsten, T. R. et al., Co-expression analysis, proteomic and metabolomic study on the impact of a Deg/HtrA protease triple mutant in Synechocystis sp. PCC 6803 exposed to temperature and high light stress. J. Proteomics 2013, 78, 294-311.
97. Wang, C. Y., Shen, R. F., Wang, C., Wang, W., Root protein profile changes induced by Al exposure in two rice cultivars differing in Al tolerance. J. Proteomics 2013, 78, 281-293.
98. Salavati, A., Khatoon, A., Nanjo, Y., Komatsu, S., Analysis of proteomic changes in roots of soybean seedlings during recovery after flooding. J. Proteomics 2012, 75, 878-893.
99. Kieffer, P., Schröder, P., Dommes, J., Hoffmann, L. et al., Proteomic and enzymatic response of poplar to cadmium stress. J. Proteomics 2009, 72, 379-396.
100. Chernov, V. M., Chernova, O. A., Medvedeva, E. S., Mouzykantov, A. A. et al., Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis. J. Proteomics 2011, 74, 2920-2936.
101. Immel, F., Renaut, J., Masfaraud, J. F., Physiological response and differential leaf proteome pattern in the European invasive Asteraceae Solidago canadensis colonizing a former cokery soil. J. Proteomics 2012, 75, 1129-1143.
102. Wang, L., Ma, H., Song, L., Shu, Y., Gu, W., Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress. J. Proteomics 2012, 75, 2109-2127.
103. Li, H., Cao, H., Wang, Y., Pang, Q. et al., Proteomic analysis of sugar beet apomictic monosomic addition line M14. J. Proteomics. 2009, 73(2), 297-308.
104. Bazargani, M. M., Sarhadi, E., Bushehri, A. A., Matros, A. et al., A proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat. J. Proteomics 2011, 74, 1959-1973.
105. Ricardo, C. P., Martins, I., Francisco, R., Sergeant, K. et al., Proteins associated with cork formation in Quercus suber L. stem tissues. J. Proteomics 2011, 74, 1266-1278.
106. Rocco, M., Corrado, G., Arena, S., D'Ambrosio, C. et al., The expression of tomato prosystemin gene in tobacco plants highly affects host proteomic repertoire. J. Proteomics 2008, 71, 176-185.
107. Bianco, L., Lopez, L., Scalone, A. G., Di Carli, M. et al., Strawberry proteome characterization and its regulation during fruit ripening and in different genotypes. J. Proteomics 2009, 72, 586-607.
108. Giraldo, E., Díaz, A., Corral, J. M., García, A., Applicability of 2-DE to assess differences in the protein profile between cold storage and not cold storage in nectarine fruits. J. Proteomics 2012, 75, 5774-5782.
109. Pan, Z., Zeng, Y., An, J., Ye, J. et al., An integrative analysis of transcriptome and proteome provides new insights into carotenoid biosynthesis and regulation in sweet orange fruits. J. Proteomics 2012, 75, 2670-2684.
110. Noah, A. M., Niemenak, N., Sunderhaus, S., Haase, C. et al., Comparative proteomic analysis of early somatic and zygotic embryogenesis in Theobroma cacao L. J. Proteomics 2013, 78, 123-133.
111. Uváčková, L., Takáč, T., Boehm, N., Obert, B., Samaj, J., Proteomic and biochemical analysis of maize anthers after cold pretreatment and induction of androgenesis reveals an important role of anti-oxidative enzymes. J. Proteomics 2012, 75, 1886-1894.
112. Du, S. J., Dong, C. J., Zhang, B., Lai, T. F. et al., Comparative proteomic analysis reveals differentially expressed proteins correlated with fuzz fiber initiation in diploid cotton (Gossypium arboreum L.). J. Proteomics 2013, 82, 113-129.
113. Kjellsen, T. D., Shiryaeva, L., Schröder, W. P., Strimbeck, G. R., Proteomics of extreme freezing tolerance in Siberian spruce (Picea obovata). J. Proteomics 2010, 73, 965-975.
114. Valero, G. J., Valledor, L., González, F. R., Navarro Cerrillo, R. M., Jorrín-Novo, J. V., Proteomic analysis of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) pollen. J. Proteomics 2012, 75, 2736-2744.
115. Wang, X. R., Liu, W. Z., Development of oil bodies in the fruit of Pistacia chinensis. Chin. Bullet Bot. 2011, 46, 665-674.
116. Christensen, J. H., Bauw, G., Welinder, K. G., Van Montagu, M., Boerjan, W., Purification and characterization of peroxidases correlated with lignification in poplar xylem. Plant Physiol. 1998, 118, 125-135.
117. Wilkins, M. R., Sanchez, J. C., Williams, K. L., Hochstrasser, D. F., Current challenges and future applications for protein maps and post-translational vector maps in proteome projects. Electrophoresis 1996, 17, 830-838.
118. Islam, N., Lonsdale, M., Upadhyaya, N. M., Higgins, T. J. et al., Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis. Proteomics 2004, 4, 1903-1908.
119. Roya, A., Rushton, P. J., Rohila, J. S., The potential of proteomics technologies for crop improvement under drought conditions. Crit. Rev. Plant Sci. 2011, 30, 471-490.
120. Tai, F. J., Yuan, Z. L., Wu, X. L., Zhao, P. F. et al., Identification of membrane proteins in maize leaves, altered in stress through polyethylene glycol treatment. Plant Omics J. 2011, 4, 250-256.