Improving gel-based proteome analysis of soluble protein extracts by heat prefractionation

Proteomics

Volumn 12, Issue 7, 2012, Pages 938-943

  Wang, Wei ^a   Wu, Xiaolin ^a   Xiong, Erhui ^a   Tai, Fuju ^a  

^a HENAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

2DE; Low abundance proteins; Prefractionation technique; Technology; Thermal denaturing

Indexed keywords

BACTERIAL PROTEIN; HEAT SHOCK PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EVALUATION; FRACTIONATION; GEL; HEAT PREFRACTIONATION; HEATING; MAIZE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN PURIFICATION;

CHEMICAL FRACTIONATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ESCHERICHIA COLI PROTEINS; HOT TEMPERATURE; PLANT PROTEINS; PROTEIN DENATURATION; PROTEIN STABILITY; PROTEOME; PROTEOMICS; SEEDS; SOLUBILITY; ZEA MAYS;

EID: 84859995893     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201100475     Document Type: Article

References (15)

1. Cravatt, B. F., Simon, G. M., Yates, J. R., The biological impact of mass-spectrometry-based proteomics. Nature 2007, 450, 991-1000.
2. Ghaemmaghami, S., Huh, W., Bower, K., Howson, R. W. et al., Global analysis of protein expression in yeast. Nature 2003, 425, 737-741.
3. Gygi, S. P., Corthals, G. L., Zhang, Y., Rochon, Y. et al., Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc. Natl. Acad. Sci. USA 2000, 97, 9390-9395.
4. Doud, M. K., Schmidt, M. W., Hines, D., Naumann, C. et al., Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE- based proteome analysis. BMC Genomics 2004, 5, 25--31.
5. Righetti, P. G., Castagna, A., Antonioli, P., Boschetti, E., Prefractionation techniques in proteome analysis: the mining tools of the third millennium. Electrophoresis 2005, 26, 297-319.
6. Hörth, P., Miller, C. A., Preckel, T., Wenz, C., Efficient fractionation and improved protein identification by peptide OFFGEL electrophoresis. Mol. Cell. Proteomics 2006, 5, 1968-1974.
7. Boudet, J., Buitink, J., Hoekstra, F. A., Rogniaux, H. et al., Comparative analysis of the heat stable proteome of radicles of Medicago truncatula seeds during germination identifies late embryogenesis abundant proteins associated with desiccation tolerance. Plant Physiol. 2006, 140, 1418-1436.
8. Kwon, S., Jung, Y., Lim, D., Proteomic analysis of heat stable proteins in Escherichia coli. BMB Rep. 2008, 41, 108-111.
9. Kochetkov G. A., Manual for Enzymology, Vysshaya Shkola, Moscow, 1980, p. 13.
10. Pusztai, A., Interactions of proteins with other polyelectrolytes in a two-phase system containing phenol and aqueous buffers at various pH values. Biochem. J. 1966, 99, 93-101.
11. Wang, W., Scali, M., Vignani, R., Spadafora, A. et al., Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds. Electrophoresis 2003, 24, 2369-2375.
12. Wu, X. L., Liu, H. Y., Wang, W., Chen, S. N. et al., Proteomic analysis of seed viability in maize. Acta Physiol. Plant. 2011, 33, 181-191.
13. Dure, L., III, Crouch, M., Harada, J., David Ho, T. H. et al., Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol. Biol. 1989, 12, 475-486.
14. Park, S. C., Lee, J. R., Shin, S. O., Park, Y. et al., Characterization of a heat stable protein with antimicrobial activity from Arabidopsis thaliana. Biochem. Biophy. Res. Commun. 2007, 362, 562-567.
15. Wang, W. X., Pelah, D., Alergand, T., Shoseyov, O. et al., Characterization of SP1, a stress-responsive, boiling-soluble, homoligomeric protein from aspen. Plant Physiol. 2002, 130, 865-875.