Removal of lipid contaminants by organic solvents from oilseed protein extract prior to electrophoresis

Analytical Biochemistry

Volumn 329, Issue 1, 2004, Pages 139-141

  Wang, Wei ^a   Vignani, Rita ^a   Scali, Monica ^a   Sensi, Elisabetta ^a   Tiberi, Pamela ^a   Cresti, Mauro ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

PROTEIN EXTRACT;

ELECTROPHORESIS;

CASTOR OIL; ORGANIC SOLVENT; VEGETABLE OIL;

AQUEOUS SOLUTION; ARTICLE; CONTAMINATION; ELECTROPHORESIS; EXTRACTION; LIPID ANALYSIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RAPESEED; SESAME; SUNFLOWER;

CASTOR; HELIANTHUS; SESAMUM INDICUM; SINAPIS ARVENSIS;

EID: 2342559149     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.02.044     Document Type: Article

References (10)

1. Huang A.H.C. Oleosins in seeds and other organs. Plant Physiol. 110:1996;1055-1061
2. Murphy D.J. Structure, function, and biogenesis of storage lipid bodies and oleosins in plants. Prog. Lipid Res. 32:1993;247-280
3. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
4. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bactriophage T4. Nature. 222:1970;680-685
5. Wang W., Scali M., Vignani R., Spadafora A., Sensi E., Mazzuca S., Cresti M. Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds. Electrophoresis. 24:2003;2369-2375
6. Hurkman M.A., Tanaka C.K. Solubilization of plant membrane proteins for analysis by two-dimensional gel electophoresis. Plant Physiol. 81:1986;802-806
7. Cabib E., Polacheck I. Protein assay for dilute solutions. Methods Enzymol. 104:1984;415-416
8. Wessel D., Flügge U.I. A method for the quantitative recovery of proteins in dilute solutions in the presence of detergents and lipids. Anal. Biochem. 138:1984;141-143
9. Folch J., Lees M., Stanley G.H.S. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:1957;497-509
10. Görg A., Obermaier C., Boguth G., Csordas A., Diaz J.J., Madjar J.J. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins. Electrophoresis. 18:1997;328-337