Evaluation of sample preparation methods for the analysis of papaya leaf proteins through two-dimensional gel electrophoresis

Phytochemical Analysis

Volumn 20, Issue 6, 2009, Pages 456-464

  Rodrigues, Silas Pessini ^a,b   Ventura, José Aires ^b,c   Zingali, R B ^a   Fernandes, P M B ^b  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b FEDERAL UNIVERSITY OF ESPÍRITO SANTO   (Brazil)

^c Fazenda Experimental Bananal do Norte   (Brazil)

Author keywords

2 DE; Carica papaya; Mass spectrometry; Plant proteomics; Protein extraction method

Indexed keywords

ACETONE; DESORPTION; ELECTROPHORESIS; EXTRACTION; FRACTIONATION; INDUCTIVELY COUPLED PLASMA; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PLANTS (BOTANY); PRECIPITATION (CHEMICAL); TRICHLOROACETIC ACID;

2-DE; CARICA PAPAYA; EXTRACTION METHOD; LEAF PROTEINS; PLANT PROTEOMICS; PROTEIN EXTRACTION; PROTEIN EXTRACTION METHOD; PROTEOMIC ANALYSIS; SAMPLE PREPARATION; SAMPLE PREPARATION METHODS;

PROTEINS;

AMMONIUM ACETATE; METHANOL; PAPAYA PROTEIN; PHENOL; TRICHLOROACETIC ACID; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ACID PRECIPITATION; ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; EXTRACTION; ISOELECTRIC FOCUSING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PAPAYA; PLANT LEAF; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEOMICS; SEPARATION TECHNIQUE; SOLUBILIZATION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CARICA PAPAYA;

EID: 73249129205     PISSN: 09580344     EISSN: 10991565     Source Type: Journal    
DOI: 10.1002/pca.1147     Document Type: Article

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