Evaluation of the effect of beetroot juice on DMBA-induced damage in liver and mammary gland of female sprague-dawley rats

Phytotherapy Research

Volumn 28, Issue 1, 2014, Pages 55-61

  Szaefer, Hanna ^a   Krajka Kuźniak, Violetta ^a   Ignatowicz, Ewa ^a   Adamska, Teresa ^a   Baer Dubowska, Wanda ^a  

^a POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

Author keywords

7,12 dimethylbenz a anthracene; beetroot juice; cytochrome P450; liver; mammary gland; phase II enzymes

Indexed keywords

ALANINE AMINOTRANSFERASE; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; BEETROOT EXTRACT; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 1B1; GAMMA GLUTAMYLTRANSFERASE; GLUTATHIONE TRANSFERASE P1; LACTATE DEHYDROGENASE; LIVER PROTECTIVE AGENT; PLANT EXTRACT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE 1; SUCCINATE DEHYDROGENASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BEETROOT; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DRUG MECHANISM; DRUG RESEARCH; DRUG SCREENING; ENZYME ACTIVITY; FEMALE; LIVER INJURY; NONHUMAN; PROTEIN EXPRESSION; RAT; TREATMENT DURATION;

ANIMALIA; RATTUS;

7,12-DIMETHYLBENZ[A]ANTHRACENE; BEETROOT JUICE; CYTOCHROME P450; LIVER; MAMMARY GLAND; PHASE II ENZYMES;

9,10-DIMETHYL-1,2-BENZANTHRACENE; ANIMALS; BETA VULGARIS; CYTOCHROME P-450 CYP1A1; CYTOCHROMES; DNA DAMAGE; FEMALE; GLUTATHIONE S-TRANSFERASE PI; GLUTATHIONE TRANSFERASE; LIVER; MAMMARY GLANDS, ANIMAL; METABOLIC DETOXICATION, DRUG; NAD(P)H DEHYDROGENASE (QUINONE); PLANT EXTRACTS; PLANT ROOTS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84892442582     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.4951     Document Type: Article

References (39)

1. Azeredo HMC,. 2009. Betalains: properties, sources, applications, and stability-a review. Int J Food Sci Technol 44: 2365-2376.
2. Benson AM, Barretto PB, Stanley JS,. 1986. Induction of DT-diaphorase by anticarcinogenic sulfur compounds in mice. J Natl Cancer Inst 76: 467-473.
3. Bhattacharya P, Keating AF,. 2012. Protective role for ovarian glutathione S-transferase isoform pi during 7,12-dimethylbenz[a]anthracene- induced ovotoxicity. Toxicol Appl Pharmacol 260: 201-208.
4. Brauze D, Widerak M, Cwykiel J, Szyfter K, Baer-Dubowska W,. 2006. The effect of aryl hydrocarbon receptor ligands on the expression of AhR, AhRR, ARNT, Hif1α,CYP1A1 and NQO1 genes in rat liver. Toxicology 167: 212-220.
5. Burke MD, Thompson S, Weaver RJ, Wolf CR, Mayer RT,. 1994. Cytochrome P450 specificities of alkoxyresorufin O-dealkylation in human and rat liver. Biochem Pharmacol 4: 923-936.
6. Coles B, Ketterer B,. 1999. The role of glutathione transferases in chemical carcinogenesis. Crit Rev Biochem Mol Biol 25: 47-70.
7. Collins AR,. 2004. The comet assay for DNA damage and repair: principles, applications and limitations. Mol Biotech 26: 249-261.
8. Ernster L,. 1967. DT-diaphorase. Methods Enzymol 10: 309-317.
9. Girolami F, Abbadessa G, Racca S, et al,. 2008. Time-dependent acetylsalicylic acid effects on liver CYP1A and antioxidant enzymes in rat model of 7,12-dimethylbenzanthracene (DMBA)-induced mammary carcinogenesis. Toxicol Lett 181: 87-92.
10. Habig WH, Pabs MJ, Jacoby WB,. 1974. Glutathione S-transferse. The first enzymatic steps in mercapturic acid formation. J Biol Chem 249: 7130-7139.
11. Han EH, Hwang YO, Jeong TC, Lee SS, Shin JG, Jeong HG,. 2007. Eugenol inhibit 7,12-dimethylbenz[a]anthracene-induced genotoxicity in MCF-7 cells: bifunctional effects on CYP1 and NAD(P)H:quinone oxidoreductase. FEBS Lett 581: 749-756.
12. Hartmann A, Agurell E, Beevers C, et al,. 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 8: 45-51.
13. Henderson CJ, Smith AG, Ure J, Brown K, Bacon EJ, Wolf CR,. 1998. Increased skin tumorigenesis in mice lacking pi class glutathione S-transferases. PNAS 95: 5275-5280.
14. Jang SM, Kim MJ, Choi MS, Kwon EY, Lee MK,. 2010. Inhibitory effects of ursolic acid on hepatic polyol pathway and glucose production in streptozotocin-induced diabetic mice. Metabolism 59: 512-519.
15. Jung KJ, Wallig MA, Singletary KW,. 2006. Purple grape juice inhibits 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumorigenesis and in vivo DMBA-DNA adduct formation. Cancer Lett 233: 279-288.
16. Kanner J, Harel S, Granit R,. 2001. Betalains: a new class of dietary cationized antioxidants. J Agric Food Chem 49: 5178-5185.
17. Kapadia GJ, Rao GS,. 2012. Anticancer effects of red beet pigments. In Red Beet Biotechnology. Food and Pharmaceutical Application, Bhagyalakshmi, Neelwarne, (ed.) Springer Sciences and Business Media: New York; 125-154.
18. Kapadia GJ, Azuine MA, Sridhar R, et al,. 2003. Chemoprevention of DMBA-induced UV-B promoted, NOR-1-induced TPA promoted skin carcinogenesis, and DEN-induced phenobarbital promoted liver tumors in mice by extract of beetroot. Pharmacol Res 47: 141-148.
19. Kawano KI, Umemura K,. 2012. Oral intake of beet extract provides protection against skin barrier impairment in hairless mice. Phytother Res doi: 10.1002/ptr.4792
20. Keum YS, Han YH, Liew C, et al,. 2006. Induction of heme oxygenase-1 (HO-1) and NAD[P]H:quinone oxidoreductase 1 (NQO1) by a phenolic antioxidant, butylated hydroxyanisole (BHA) and its metabolite, tert-buthylhydroquinone (tBHQ) in primary-cultured human and rat hepatocytes. Pharm Res 23: 2586-2594.
21. Khan MI, Joseph KMD, Muralidhara, Ramesh HP, Giridhar P, Ravishankar GA,. 2011. Acute, subacute and subchronic safety assessment of betalains rich Rivina humilis L. berry juice in rats. Food Chem Toxicol 49: 3154-3157.
22. Klewicka E, Nowak A, Zduńczyk Z, Juśkiewicz J, Cukrowska B,. 2012. Protective effect of lactofermented red beetroot juice against aberrant crypt foci formation, genotoxicity of fecal water and oxidative stress induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine in rats model. Environ Toxicol Pharmacol 34: 895-904.
23. Krajka-Kuźniak V, Szaefer H, Baer-Dubowska W,. 2004. Modulation of 3-methylcholanthrene-induced rat hepatic and renal cytochrome P450 and phase II enzymes by plant phenols: protocatechuic and tannic acids. Toxicol Lett 152: 117-126.
24. Krajka-Kuźniak V, Kaczmarek J, Baer-Dubowska W,. 2008. Effect of naturally occurring phenolic acids on the expression of glutatione S-transferase isozymes in the rat. Food Chem Toxicol 46: 1097-1102.
25. Krajka-Kuźniak V, Szaefer H, Ignatowicz E, Adamska T, Baer-Dubowska W,. 2012. Beetroot juice protects against N-nitrosodiethylamine- induced liver injury in rats. Food Chem Toxicol 50: 2027-2033.
26. Kujawska M, Ignatowicz E, Murias M, Ewertowska M, Mikołajczyk K, Jodynis-Liebert J,. 2009. Protective effect of red beetroot against carbon tetrachloride- and N-nitrosodiethylamine-induced oxidative stress in rats. J Agric Food Chem 57: 2570-2575.
27. Laemmli UK,. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
28. Lambard SE, Burnett AK, Wolf CR, Craft JA,. 1991. The role of specific cytochromes P450 in the formation of 7,12-dimethylbenz(a)anthracene-protein adducts in rat liver microsomes in vitro. Biochem Pharmacol 42: 1529-1535.
29. Lee CH-H, Wettasinghe M, Bolling B, Ji L-L, Parkin K,. 2005. Betalains, phase II enzyme-inducing components from red beetroot (Beta vulgaris L.) extracts. Nutr Cancer 53: 91-103.
30. Long II DJ, Waikel RL, Wang X-J, Roop DR, Jaiswal AK,. 2001. NAD(P)H:quinone oxidoreductase 1 deficiency and increased susceptibility to 7,12-dimethylbenz[a]anthracene-induced carcinogenesis in mouse skin. J Nat Cancer Inst 93: 1166-1170.
31. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ,. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
32. Nijhoff WA, Groen GM, Peters WHM,. 1993. Induction of rat hepatic and intestinal glutathione S-transferases and glutathione by dietary naturally occurring anticarcinogens. Inter J Oncol 3: 1131-1139.
33. Nilson T., 1970. Studies into the pigments in beet root (Beta vulgaris var rubra L.). Lantbrukshogsk Ann 36: 179-219.
34. Sreekanth D, Arunasree MK, Roy KR, Chandramohan Reddy T, Reddy GV, Reddanna P,. 2007. Betanin a betacyanin pigment purified from fruits of Opuntia ficus-indica induces apoptosis in human chronic myeloid leukemia Cell line-K562. Phytomedicine 14: 739-746.
35. Szaefer H, Krajka-Kuźniak V, Ignatowicz I, Adamska T, Baer-Dubowska W,. 2011. Chokeberry (Aronia melanocarpa) juice modulates 7,12-dimethylbenz[a]anthracene induced hepatic but not mammary gland phase I and II enzymes in female rats. Envirom Toxicol Pharm 31: 339-346.
36. Towbin H, Staehelin T, Gordon J,. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application. Proc Natl Acad Sci USA 76: 4350-4354.
37. Wootton-Beard PC, Ryan L,. 2011. A beetroot juice shot is a significant and convenient source of bioaccessible antioxidants. J Funkt Foods 3: 329-334.
38. Zielińska-Przyjemska M, Olejnik A, Dobrowolska-Zachwieja A, Grajek W,. 2009. In vitro effects of beetroot juice and chips on oxidative metabolism and apoptosis in neutrophils from obese individuals. Phytother Res 23: 49-55.
39. Zielińska-Przyjemska M, Olejnik A, Kostrzewa A, Łuczak M, Jagodziński PP, Baer-Dubowska W,. 2012. The beetroot component betanin modulates ROS production, DNA damage and apoptosis in human polymorphonuclear neutrophils. Phytother Res 26: 845-852.