The beetroot component betanin modulates ROS production, DNA damage and apoptosis in human polymorphonuclear neutrophils

Phytotherapy Research

Volumn 26, Issue 6, 2012, Pages 845-852

  Zielińska Przyjemska, Małgorzata ^a   Olejnik, Anna ^b   Kostrzewa, Artur ^a   Śuczak, Michał ^a   Jagodziński, Paweł P ^a   Baer Dubowska, Wanda ^a  

^a POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

^b POZNA UNIVERSITY OF LIFE SCIENCES   (Poland)

Author keywords

apoptosis; betanin (5 O glucose betanidine); DNA damage; polymorphonuclear neutrophils; reactive oxygen species

Indexed keywords

BETANIN; CASPASE 3; PHORBOL 13 ACETATE 12 MYRISTATE; PLANT MEDICINAL PRODUCT; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

AEROBIC METABOLISM; ANTIINFLAMMATORY ACTIVITY; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; BEETROOT; CHEMOLUMINESCENCE; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DAMAGE; DRUG EFFECT; DRUG STRUCTURE; ENZYME ACTIVATION; ENZYME ACTIVITY; FEMALE; HUMAN; HUMAN CELL; IN VITRO STUDY; INHIBITION KINETICS; MALE; NEUTROPHIL; PROTEIN CLEAVAGE; UPREGULATION; WESTERN BLOTTING;

ANTIOXIDANTS; APOPTOSIS; BETA VULGARIS; BETACYANINS; CASPASE 3; CELL SURVIVAL; COMET ASSAY; DNA DAMAGE; DRUG EVALUATION, PRECLINICAL; ENZYME ACTIVATION; HUMANS; LUMINESCENT MEASUREMENTS; NEUTROPHILS; REACTIVE OXYGEN SPECIES; TETRADECANOYLPHORBOL ACETATE; TIME FACTORS;

EID: 84861822114     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.3649     Document Type: Article

References (31)

1. Babior BM,. 2000. Phagocytes and oxidative stress. Am J Med 109: 33-44.
2. Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M,. 1983. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J Immunol 130: 1910-1917.
3. Berger SL, Kouzarides T, Shiekhattar R, Shilatifard A,. 2009. An operational definition of epigenetics. Genes Dev 23: 781-783.
4. Cai Y, Sun M, Corke H,. 2003. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem 51: 2288-2294.
5. Collins AR,. 2004. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 26: 249-261.
6. Duthie SJ, Ma A, Ross MA, Collins AR,. 1996. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res 56: 1291-1295.
7. Fadeel B, Ahlin A, Henter JI, Orrenius S, Hampton MB,. 1998. Involvement of caspases in neutrophil apoptosis: regulation by reactive oxygen species. Blood 92: 4808-4818.
8. Ferguson LR,. 2001. Role of plant polyphenols in genomic stability. Mutat Res 475: 89-111.
9. Groemping Y, Lapouge K, Smerdon SJ, Rittinger K,. 2003. Molecular basis of phosphorylation-induced activation of the NADPH oxidase. Cell 113: 343-355.
10. Hampton MB, Orrenius S,. 1997. Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis. FEBS Lett 414: 552-556.
11. Hampton MB, Stamenkovic I, Winterbourn CC,. 2002. Interaction with substrate sensitises caspase-3 to inactivation by hydrogen peroxide. FEBS Lett 517: 229-232.
12. Hartmann A, Agurell E, Beevers C, et al,. 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 18: 45-51.
13. Ignatowicz E, Balana B, Vulimiri SV, Szaefer H, Baer-Dubowska W,. 2003. The effect of plant phenolics on the formation of 7,12-dimethylbenz[a] anthracene-DNA adducts and TPA-stimulated polymorphonuclear neutrophils chemiluminescence in vitro. Toxicology 189: 199-209.
14. Jiang ZY, Woolard ACS, Wolff SP,. 1990. Hydrogen peroxide production during experimental protein glycation. FEBS Lett 268: 69-71.
15. Kanner J, Harel S, Granit R,. 2001. Betalains-a new class of dietary cationized antioxidants. J Agric Food Chem 49: 5178-5185.
16. Kapadia GJ, Azuine MA, Sridhar R, et al,. 2003. Chemoprevention of DMBA-induced UV-B promoted, NOR-1-induced TPA promoted skin carcinogenesis, and DEN-induced phenobarbital promoted liver tumors in mice by extract of beetroot. Pharmacol Res 47: 141-148.
17. Kujawska M, Ignatowicz E, Murias M, Ewertowska M, Mikołajczyk K, Jodynis-Liebert J,. 2009. Protective effect of red beetroot against carbon tetrachloride- and N-nitrosodiethylamine-induced oxidative stress in rats. J Agric Food Chem 57: 2570-2575.
18. Mao JH, Sun XY, Liu JX, et al,. 2010. As4S4 targets RING-type E3 ligase c-CBL to induce degradation of BCR-ABL in chronic myelogenous leukemia. Proc Natl Acad Sci USA 107: 21683-21688.
19. McPhail LC, Clayton CC, Snyderman R,. 1984. The NADPH oxidase of human polymorphonuclear leukocytes. Evidence for regulation by multiple signals. J Biol Chem 1259: 5768-5775.
20. Ohshima H, Tazawa H, Sylla BS, Sawa T,. 2005. Prevention of human cancer by modulation of chronic inflammatory processes. Mutat Res 591: 110-122.
21. Olive PL, Banáth JP,. 2006. The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 1: 23-29.
22. Reddy MK, Alexander-Lindo RL, Nair MG,. 2005. Relative inhibition of lipid peroxidation, cyclooxygenase enzymes, and human tumor cell proliferation by natural food colors. J Agric Food Chem 53: 9268-9273.
23. Sharif T, Auger C, Alhosin M, et al,. 2010. Red wine polyphenols cause growth inhibition and apoptosis in acute lymphoblastic leukaemia cells by inducing a redox-sensitive up-regulation of p73 and down-regulation of UHRF1. Eur J Cancer 46: 983-994.
24. Strack D, Vogt T, Schliemann W,. 2003. Recent advances in betalain research. Phytochemistry 62: 247-269.
25. Tesoriere L, Allegra M, Butera D, Livrea MA,. 2004. Absorption, excretion, and distribution of dietary antioxidant betalains in LDLs: potential health effects of betalains in humans. Am J Clin Nutr 80: 941-945.
26. Vissers MC, Wilkie RP,. 2007. Ascorbate deficiency results in impaired neutrophil apoptosis and clearance and is associated with up-regulation of hypoxia-inducible factor 1alpha. J Leukoc Biol 81: 1236-1244.
27. Watts AR, Lennard MS, Mason SL, Tucker GT, Woods HF,. 1993. Beeturia and the biological fate of beetroot pigments. Pharmacogenetics 3: 302-311.
28. Wilkie RP, Vissers MC, Dragunow M, Hampton MB,. 2007. A functional NADPH oxidase prevents caspase involvement in the clearance of phagocytic neutrophils. Infect Immun 75: 3256-3263.
29. Zielińska-Przyjemska M, Ignatowicz E,. 2008. Citrus fruit flavonoids influence on neutrophil apoptosis and oxidative metabolism. Phytother Res 22: 1557-1562.
30. Zielińska-Przyjemska M, Olejnik A, Dobrowolska-Zachwieja A, Grajek W,. 2009. In vitro effects of beetroot juice and chips on oxidative metabolism and apoptosis in neutrophils from obese individuals. Phytother Res 23: 49-55.
31. Zielińska-Przyjemska M, Wiktorowicz K,. 2006. An in vitro study of the protective effect of the flavonoid silydianin against reactive oxygen species. Phytother Res 20: 115-119.