Targeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli

FEBS Letters

Volumn 588, Issue 2, 2014, Pages 247-252

  Hwang, Peter M ^a   Pan, Jonathan S ^a   Sykes, Brian D ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Chemical cleavage; Fusion protein; Inclusion body

Indexed keywords

2 (2 NITROPHENYL) 3 METHYL 3 BROMOINDOLENINE; 2 NITRO 5 THIOCYANATOBENZOIC ACID; ASPARAGINE; ASPARTIC ACID; BENZOIC ACID; BIOTIN; CHEMICAL COMPOUND; CYANOGEN BROMIDE; CYSTEINE; GLUTAMIC ACID; GLUTAMINE; GUANIDINE; HISTIDINE; HYBRID PROTEIN; METAL ION; METHIONINE; PAGP STEROID DELTA ISOMERASE; PALLADIUM; PURF STEROID DELTA ISOMERASE; STEROID DELTA ISOMERASE; STREPTAVIDIN; TRPLE STEROID DELTA ISOMERASE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG; UREA;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; BIOCATALYSIS; BIOTINYLATION; CELL INCLUSION; ESCHERICHIA COLI; HYDROGEN BOND; HYDROPHOBICITY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IN VITRO STUDY; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; OPERON; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN REFOLDING; PROTEIN STRUCTURE; PROTEIN TARGETING; REVIEW; SOLUBILIZATION; TRPE GENE;

ESCHERICHIA COLI;

CHEMICAL CLEAVAGE; FUSION PROTEIN; INCLUSION BODY;

ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; HUMANS; INCLUSION BODIES; PROTEIN DENATURATION; RECOMBINANT FUSION PROTEINS;

EID: 84892370557     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2013.09.028     Document Type: Review

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