Application of Ni(II)-assisted peptide bond hydrolysis to non-enzymatic affinity tag removal

PLoS ONE

Volumn 7, Issue 5, 2012, Pages

  Kopera, Edyta ^a   Belczyk Ciesielska, Agnieszka ^a   Bal, Wojciech ^a  

^a INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

DETERGENT; HYBRID PROTEIN; NICKEL; HYDROXYLAMINE; OLIGOPEPTIDE; UBIQUITIN;

AFFINITY TAG REMOVAL; AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; CHEMICAL BOND; CHEMICAL PROCEDURES; CONTROLLED STUDY; HYDROLYSIS; NONHUMAN; PEPTIDE BOND; PH; PROTEIN BINDING; TEMPERATURE; AFFINITY LABELING; CHEMISTRY; ENZYME SPECIFICITY; HUMAN; ISOLATION AND PURIFICATION; PROTEIN DEGRADATION; PROTEIN MOTIF; SOLUTION AND SOLUBILITY;

AFFINITY LABELS; AMINO ACID MOTIFS; HUMANS; HYDROLYSIS; HYDROXYLAMINE; NICKEL; OLIGOPEPTIDES; PROTEOLYSIS; SOLUTIONS; SUBSTRATE SPECIFICITY; UBIQUITIN;

EID: 84860524464     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0036350     Document Type: Article

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