A tandem affinity tag for two-step purification under fully denaturing conditions: Application in ubiquitin profiling complex identification combined with in vivo cross-linking

Molecular and Cellular Proteomics

Volumn 5, Issue 4, 2006, Pages 737-748

  Tagwerker, Christian ^a,b   Flick, Karin ^a   Cui, Meng ^a   Guerrero, Cortnie ^a   Dou, Yimeng ^c   Auer, Bernhard ^b   Baldi, Pierre ^a,c   Huang, Lan ^a   Kaiser, Peter ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF INNSBRUCK   (Austria)

^c 5226 Donald Bren Hall   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; HISTIDINE; HYDROLASE; NITROGEN; RESIN; SIGNAL PEPTIDE; STREPTAVIDIN; UBIQUITIN; UREA;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BIOTINYLATION; CELL ADHESION; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; IN VIVO STUDY; MAMMAL CELL; MASS SPECTROMETRY; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN DENATURATION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEOMICS; SEPARATION TECHNIQUE; SEQUENCE ANALYSIS; TANDEM REPEAT; YEAST CELL;

AFFINITY LABELS; AMINO ACID SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEIN DENATURATION; PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; UBIQUITIN;

MAMMALIA;

EID: 33645703441     PISSN: 15359476     EISSN: None     Source Type: Journal    
DOI: 10.1074/mcp.M500368-MCP200     Document Type: Article

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