Improved efficiency of site-specific copper(II) ion-catalysed protein cleavage effected by mutagenesis of cleavage site

Protein Engineering

Volumn 13, Issue 3, 2000, Pages 201-206

  Humphreys, David P ^a   King, Lloyd M ^a   West, Shauna M ^a   Chapman, Andrew P ^a   Sehdev, Mukesh ^a   Redden, Matthew W ^a   Glover, David J ^a   Smith, Bryan J ^a   Stephens, Paul E ^a  

^a UCB PHARMA   (Belgium)

Author keywords

Copper(II); Cupric; Fab'; FLAG peptide; Protein cleavage

Indexed keywords

COPPER ION; HYBRID PROTEIN; NICKEL;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; MASS SPECTROMETRY; MOLECULAR WEIGHT; MUTAGENESIS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; TEMPERATURE SENSITIVITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CONSERVED SEQUENCE; COPPER; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FAB FRAGMENTS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NICKEL; OLIGODEOXYRIBONUCLEOTIDES; PLASMIDS; RECOMBINANT FUSION PROTEINS; THERMODYNAMICS;

EID: 0034054344     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/13.3.201     Document Type: Article

References (11)

1. Allen, G. and Campbell, R.O. (1996) Int. J. Pept. Protein Res., 48, 265-273.
2. Cotton, F.A. and Wilkinson, G. (eds) (1972) Advanced Inorganic Chemistry. Interscience, New York.
3. Dawson, R.M.C., Elliott, D.C., Elliott, W.H. and Jones, K.M. (eds) (1986) Data for Biochemical Research. Oxford Science Publications, Oxford.
4. Humphreys, D.P., Chapman, A.P., Reeks, D.G., Lang, V. and Stephens, P.E. (1997) J. Immunol. Methods, 209, 193-202.
5. Humphreys, D.P. et al. (1998) J. Immunol. Methods, 217, 1-10.
6. Humphreys, D.P., Smith, B.J., King, L.M., West, S.M., Reeks, D.G. and Stephens, P.E. (1999) Protein Engng, 12, 179-184.
7. Irving, H., Williams, R.P.J. (1953) J. Chem. Soc., 3192-3210.
8. Samuni, A., Chevion, M. and Czapski, G. (1981) J. Biol. Chem., 256, 12632-12635.
9. Smith, M.A., Easton, M., Everett, P., Lewis, G., Payne, M., Riveros-Moreno, V. and Allen, G. (1996) Int. J. Pept. Protein Res., 48, 48-55.
10. Tang, S.S., Lin, C.C. and Chang, G.G. (1996) Free Rad. Biol. Med., 21, 955-964.
11. Wada, K.N., Wada, Y., Doi, H., Ishibashi, F., Gojobori, T. and Ikemura, T. (1991) Nucleic Acids Res., 19, 1981-1986.