Optimization of the hydroxylamine cleavage of an expressed fusion protein to produce recombinant human insulin-like growth factor (IGF)-I

Biotechnology and Bioengineering

Volumn 50, Issue 3, 1996, Pages 265-272

  Milner, Steven J ^a   Thomas, Sonia M ^b   Ballard, F John ^a   Francis, Geoffrey L ^a  

^a Div of Hlth Sciences and Nutrition   (Australia)

^b GroPep Ltd   (Australia)

Author keywords

chemical cleavage; hydroxylamine; insulin like growth factor I; protein modification; recombinant fusion protein

Indexed keywords

AMINES; BIOASSAY; CHEMICAL MODIFICATION; CHEMICAL REACTIONS; CHROMATOGRAPHY; COLIFORM BACTERIA; GENETIC ENGINEERING; ION EXCHANGE; PH EFFECTS; THERMAL EFFECTS;

CHEMICAL CLEAVAGE REACTION; GENE FUSION TECHNOLOGY; HYDROXAMIC ACID; HYDROXYLAMINE CLEAVAGE; INSULIN LIKE GROWTH FACTOR;

PROTEINS;

HYBRID PROTEIN; HYDROXYLAMINE; SOMATOMEDIN C;

ARTICLE; DNA CLEAVAGE; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; GENETIC HETEROGENEITY; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; NONHUMAN; PROTEIN FOLDING; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

ESCHERICHIA COLI;

EID: 0030570130     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960505)50:3<265::AID-BIT5>3.0.CO;2-E     Document Type: Article

References (18)

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