Cleavage of recombinant proteins at poly-His sequences by Co(II) and Cu(II)

Protein Science

Volumn 16, Issue 8, 2007, Pages 1751-1761

  Andberg, Martina ^a   Jäntti, Jussi ^a,c   Heilimo, Sara ^a   Pihkala, Päivi ^a   Paananen, Arja ^a   Koskinen, Ari M P ^b   Söderlund, Hans ^a   Linder, Markus B ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b AALTO UNIVERSITY   (Finland)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Artificial protease; Co(II); Cu(II); His tag; Protein cleavage; Protein stability

Indexed keywords

ASCORBIC ACID; COPPER ION; CUPRIC ION; FERROUS ION; HISTIDINE; HYBRID PROTEIN; HYDROGEN PEROXIDE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; GEL ELECTROPHORESIS; MASS SPECTROMETRY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; REACTION TIME; TEMPERATURE SENSITIVITY;

ASCORBIC ACID; COBALT; COPPER; HISTIDINE; HYDROGEN PEROXIDE; PEPTIDE FRAGMENTS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 34547574316     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.072846407     Document Type: Article

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