Production and isotope labeling of antimicrobial peptides in Escherichia coli by means of a novel fusion partner that enables high-yield insoluble expression and fast purification

Journal of Peptide Science

Volumn 15, Issue 4, 2009, Pages 278-284

  Vidovic, Verica ^a   Prongidi Fix, Lydia ^a   Bechinger, Burkhard ^a   Werten, Sebastiaan ^a  

^a UMR 7177   (France)

Author keywords

Antimicrobial peptide; Bacterial expression; High throughput; NMR

Indexed keywords

ASPARTIC ACID; HYBRID PROTEIN; POLYPEPTIDE ANTIBIOTIC AGENT; PROLINE; PROTEIN LAH4; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR TAF12; UNCLASSIFIED DRUG; ANTIMICROBIAL CATIONIC PEPTIDE; ISOTOPE; NITROGEN; RECOMBINANT DNA; TAF12 PROTEIN, HUMAN; TATA BINDING PROTEIN ASSOCIATED FACTOR;

AFFINITY LABELING; ARTICLE; CHROMATOGRAPHY; DIMERIZATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FUSION GENE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PURIFICATION; RECOMBINANT DNA TECHNOLOGY; SOLUBILIZATION; AMINO ACID SEQUENCE; BIOSYNTHESIS; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

AMINO ACID SEQUENCE; ANTIMICROBIAL CATIONIC PEPTIDES; BASE SEQUENCE; DNA, RECOMBINANT; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; ISOTOPES; MOLECULAR SEQUENCE DATA; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TATA-BINDING PROTEIN ASSOCIATED FACTORS;

EID: 65349123502     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1112     Document Type: Article

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