A PagP fusion protein system for the expression of intrinsically disordered proteins in Escherichia coli

Protein Expression and Purification

Volumn 85, Issue 1, 2012, Pages 148-151

  Hwang, Peter M ^a   Pan, Jonathan S ^a   Sykes, Brian D ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Cyanogen bromide cleavage; Fusion protein expression

Indexed keywords

ACYLTRANSFERASE; CYANOGEN BROMIDE; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; PAGP PROTEIN, E COLI; TROPONIN I;

AMINO ACID SEQUENCE; ARTICLE; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; CLONING, MOLECULAR; CYANOGEN BROMIDE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; INCLUSION BODIES; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; TROPONIN I;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 84864943653     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.07.007     Document Type: Article

References (16)

1. S. Takeda, A. Yamashita, K. Maeda, and Y. Maeda Structure of the core domain of human cardiac troponin in the Ca2+-saturated form Nature 424 2003 35 41 (Pubitemid 36834832)
2. R.J. Solaro, P. Rosevear, and T. Kobayashi The unique functions of cardiac troponin I in the control of cardiac muscle contraction and relaxation Biochem. Biophys. Res. Commun. 369 2008 82 87
3. A. Kuliopulos, and C.T. Walsh Production, purification, and cleavage of tandem repeats of recombinant peptides J. Am. Chem. Soc. 116 1994 4599 4607 (Pubitemid 24255820)
4. P.M. Hwang, W.Y. Choy, E.I. Lo, L. Chen, J.D. Forman-Kay, C.R.H. Raetz, G.G. Prive, R.E. Bishop, and L.E. Kay Solution structure and dynamics of the outer membrane enzyme PagP by NMR Proc. Natl. Acad. Sci. USA 99 2002 13560 13565 (Pubitemid 35215421)
5. S.K. Buchanan Beta-barrel proteins from bacterial outer membranes: Structure, function and refolding Curr. Opin. Struct. Biol. 9 1999 455 461 (Pubitemid 29377888)
6. D. Shore, J. Langowski, and R.L. Baldwin DNA flexibility studied by covalent closure of short fragments into circles Proc. Natl. Acad. Sci. USA 78 1981 4833 4837 (Pubitemid 12225575)
7. R.E. Bishop, H.S. Gibbons, T. Guina, M.S. Trent, S.I. Miller, and C.R.H. Raetz Transfer of palmitate from phospholipids to lipid A in outer membranes of Gram-negative bacteria EMBO J. 19 2000 5071 5080
8. C. Michaux, N.C. Pomroy, and G.G. Prive Refolding SDS-denatured proteins by the addition of amphipathic cosolvents J. Mol. Biol. 375 2008 1477 1488
9. J.A. Cuesta-Seijo, C. Neale, M.A. Khan, J. Moktar, C.D. Tran, R.E. Bishop, R. Pomes, and G.G. Prive PagP crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler Structure 18 2010 1210 1219
10. J.C. Rodriguez, L. Wong, and P.A. Jennings The solvent in CNBr cleavage reactions determines the fragmentation efficiency of ketosteroid isomerase fusion proteins used in the production of recombinant peptides Protein Expr. Purif. 28 2003 224 231 (Pubitemid 36562411)
11. W. Vogt Oxidation of methionyl residues in proteins - tools, targets, and reversal Free Radical Biol. Med. 18 1995 93 105
12. Y.L. Jin, Y.Y. Huang, Y.F. Xie, W.B. Hu, F.Y. Wang, G.Q. Liu, and R. Zhao Cyclic interconversion of methionine containing peptide between oxidized and reduced phases monitored by reversed-phase HPLC and ESI-MS/MS Talanta 89 2012 531 536
13. C.H. Li Iodination of tyrosine groups in serum albumin and pepsin J. Am. Chem. Soc. 67 1945 1065 1069
14. N.M. Alexander Oxidative cleavage of tryptophanyl peptide-bonds during chemical-catalyzed and peroxidase-catalyzed iodinations J. Biol. Chem. 249 1974 1946 1952
15. W.H. Guo, L. Cao, Z.J. Jia, G. Wu, T. Li, F.X. Lu, and Z.X. Lu High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners Protein Expr. Purif. 77 2011 185 192
16. J.G. Marblestone, S.C. Edavettal, Y. Lim, P. Lim, X. Zuo, and T.R. Butt Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO Protein Sci. 15 2006 182 189 (Pubitemid 43004245)