Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics

Journal of Proteome Research

Volumn 12, Issue 12, 2013, Pages 5666-5680

  Walmsley, Scott J ^a   Rudnick, Paul A ^b   Liang, Yuxue ^b   Dong, Qian ^b   Stein, Stephen E ^b   Nesvizhskii, Alexey I ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

digestion; endoprotease specificity; label free quantification; mass spectrometry; missed cleavages; peptide abundance; proteomics; statistical analysis; trypsin; variability

Indexed keywords

HUMAN SERUM ALBUMIN; TRYPSIN; PEPTIDE FRAGMENT; SERUM ALBUMIN;

ARTICLE; COW; DIGESTION; HUMAN; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEOMICS; QUALITY CONTROL; REVERSED PHASE LIQUID CHROMATOGRAPHY; SPECIES COMPARISON; STATISTICAL ANALYSIS; SWINE; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; ANIMAL; BOVINAE; CHEMISTRY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PRINCIPAL COMPONENT ANALYSIS; REPRODUCIBILITY; SPECIES DIFFERENCE; STANDARDS;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHROMATOGRAPHY, REVERSE-PHASE; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PRINCIPAL COMPONENT ANALYSIS; PROTEOLYSIS; PROTEOMICS; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN; SPECIES SPECIFICITY; SWINE; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84890105210     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr400611h     Document Type: Article

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