Consecutive proteolytic digestion in an enzyme reactor increases depth of proteomic and phosphoproteomic analysis

Analytical Chemistry

Volumn 84, Issue 6, 2012, Pages 2631-2637

  Wiśniewski, Jacek R ^a   Mann, Matthias ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADDITIONAL SAMPLES; ENZYME REACTORS; EXCELLENT PERFORMANCE; PHOSPHORYLATION SITES; PROTEOLYTIC DIGESTION; PROTEOMIC ANALYSIS; SAMPLE DIGESTION; SAMPLE PREPARATION; SODIUM DODECYL SULFATE; SPECTROMETRIC MEASUREMENTS; TRYPTIC DIGESTION; ULTRAFILTRATION DEVICES;

ENZYMES; PHOSPHORYLATION; SODIUM; SODIUM SULFATE; ULTRAFILTRATION;

PEPTIDES;

METALLOPROTEINASE; PEPTIDYL LYS METALLOENDOPEPTIDASE; PEPTIDYL-LYS METALLOENDOPEPTIDASE; PHOSPHOPROTEIN; TRYPSIN;

ANIMAL; ARTICLE; C57BL MOUSE; CHEMISTRY; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOUSE; PHOSPHORYLATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; ULTRAFILTRATION;

ANIMALS; CHROMATOGRAPHY, LIQUID; HELA CELLS; HUMANS; METALLOENDOPEPTIDASES; MICE; MICE, INBRED C57BL; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; TRYPSIN; ULTRAFILTRATION;

EID: 84858725817     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac300006b     Document Type: Article

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