메뉴 건너뛰기




Volumn 12, Issue 8, 2013, Pages 3631-3641

Cutting edge proteomics: Benchmarking of six commercial trypsins

Author keywords

artifacts; digestion; label free and TMT quantitation; mass spectrometry; proteomics; trypsin; tyrosine sulfation

Indexed keywords

ASPARAGINE; TRYPSIN; TYROSINE;

EID: 84881166708     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr4001465     Document Type: Article
Times cited : (34)

References (32)
  • 4
    • 3042815334 scopus 로고    scopus 로고
    • Trypsin cleaves exclusively C-terminal to arginine and lysine residues
    • Olsen, J. V.; Ong, S. E.; Mann, M. Trypsin cleaves exclusively C-terminal to arginine and lysine residues Mol. Cell. Proteomics 2004, 3 (6) 608-14
    • (2004) Mol. Cell. Proteomics , vol.3 , Issue.6 , pp. 608-614
    • Olsen, J.V.1    Ong, S.E.2    Mann, M.3
  • 5
    • 34848903890 scopus 로고    scopus 로고
    • The implications of proteolytic background for shotgun proteomics
    • Picotti, P.; Aebersold, R.; Domon, B. The implications of proteolytic background for shotgun proteomics Mol. Cell. Proteomics 2007, 6 (9) 1589-98
    • (2007) Mol. Cell. Proteomics , vol.6 , Issue.9 , pp. 1589-1598
    • Picotti, P.1    Aebersold, R.2    Domon, B.3
  • 6
    • 84855877960 scopus 로고    scopus 로고
    • Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics
    • Burkhart, J. M.; Schumbrutzki, C.; Wortelkamp, S.; Sickmann, A.; Zahedi, R. P. Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics J. Proteomics 2012, 75 (4) 1454-62
    • (2012) J. Proteomics , vol.75 , Issue.4 , pp. 1454-1462
    • Burkhart, J.M.1    Schumbrutzki, C.2    Wortelkamp, S.3    Sickmann, A.4    Zahedi, R.P.5
  • 7
    • 0025081420 scopus 로고
    • Recognition of trypsin autolysis products by high-performance liquid chromatography and mass spectrometry
    • Vestling, M. M.; Murphy, C. M.; Fenselau, C. Recognition of trypsin autolysis products by high-performance liquid chromatography and mass spectrometry Anal. Chem. 1990, 62 (21) 2391-4
    • (1990) Anal. Chem. , vol.62 , Issue.21 , pp. 2391-2394
    • Vestling, M.M.1    Murphy, C.M.2    Fenselau, C.3
  • 8
    • 34548183872 scopus 로고    scopus 로고
    • Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
    • Rappsilber, J.; Mann, M.; Ishihama, Y. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips Nat. Protoc. 2007, 2 (8) 1896-906
    • (2007) Nat. Protoc. , vol.2 , Issue.8 , pp. 1896-1906
    • Rappsilber, J.1    Mann, M.2    Ishihama, Y.3
  • 9
  • 11
    • 77955643959 scopus 로고    scopus 로고
    • The minotaur proteome: Avoiding cross-species identifications deriving from bovine serum in cell culture models
    • Bunkenborg, J.; Garcia, G. E.; Paz, M. I.; Andersen, J. S.; Molina, H. The minotaur proteome: avoiding cross-species identifications deriving from bovine serum in cell culture models Proteomics 2010, 10 (16) 3040-4
    • (2010) Proteomics , vol.10 , Issue.16 , pp. 3040-3044
    • Bunkenborg, J.1    Garcia, G.E.2    Paz, M.I.3    Andersen, J.S.4    Molina, H.5
  • 12
    • 84865553658 scopus 로고    scopus 로고
    • Data extraction from proteomics raw data: An evaluation of nine tandem MS tools using a large Orbitrap data set
    • Mancuso, F.; Bunkenborg, J.; Wierer, M.; Molina, H. Data extraction from proteomics raw data: An evaluation of nine tandem MS tools using a large Orbitrap data set J. Proteomics 2012, 75 (17) 5293-5303
    • (2012) J. Proteomics , vol.75 , Issue.17 , pp. 5293-5303
    • Mancuso, F.1    Bunkenborg, J.2    Wierer, M.3    Molina, H.4
  • 15
    • 12444345515 scopus 로고    scopus 로고
    • Tandem mass tags: A novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS
    • Thompson, A.; Schafer, J.; Kuhn, K.; Kienle, S.; Schwarz, J.; Schmidt, G.; Johnstone, R.; Neumann, T.; Hamon, C. Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS Anal. Chem. 2003, 75 (8) 1895-904
    • (2003) Anal. Chem. , vol.75 , Issue.8 , pp. 1895-1904
    • Thompson, A.1    Schafer, J.2    Kuhn, K.3    Kienle, S.4    Schwarz, J.5    Schmidt, G.6    Johnstone, R.7    Neumann, T.8    Hamon, C.9
  • 16
    • 33750539321 scopus 로고    scopus 로고
    • Human cationic trypsinogen is sulfated on Tyr154
    • Sahin-Toth, M.; Kukor, Z.; Nemoda, Z. Human cationic trypsinogen is sulfated on Tyr154 FEBS J. 2006, 273 (22) 5044-50
    • (2006) FEBS J. , vol.273 , Issue.22 , pp. 5044-5050
    • Sahin-Toth, M.1    Kukor, Z.2    Nemoda, Z.3
  • 17
    • 37849045546 scopus 로고    scopus 로고
    • Mass spectrometric detection of tyrosine sulfation in human pancreatic trypsinogens, but not in tumor-associated trypsinogen
    • Itkonen, O.; Helin, J.; Saarinen, J.; Kalkkinen, N.; Ivanov, K. I.; Stenman, U. H.; Valmu, L. Mass spectrometric detection of tyrosine sulfation in human pancreatic trypsinogens, but not in tumor-associated trypsinogen FEBS J. 2008, 275 (2) 289-301
    • (2008) FEBS J. , vol.275 , Issue.2 , pp. 289-301
    • Itkonen, O.1    Helin, J.2    Saarinen, J.3    Kalkkinen, N.4    Ivanov, K.I.5    Stenman, U.H.6    Valmu, L.7
  • 19
    • 0035298820 scopus 로고    scopus 로고
    • Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode
    • Steen, H.; Kuster, B.; Fernandez, M.; Pandey, A.; Mann, M. Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode Anal. Chem. 2001, 73 (7) 1440-8
    • (2001) Anal. Chem. , vol.73 , Issue.7 , pp. 1440-1448
    • Steen, H.1    Kuster, B.2    Fernandez, M.3    Pandey, A.4    Mann, M.5
  • 20
    • 4444337337 scopus 로고    scopus 로고
    • Protein tyrosine-O-sulfation analysis by exhaustive product ion scanning with minimum collision offset in a nanoESI Q-TOF tandem mass spectrometer
    • Salek, M.; Costagliola, S.; Lehmann, W. D. Protein tyrosine-O-sulfation analysis by exhaustive product ion scanning with minimum collision offset in a nanoESI Q-TOF tandem mass spectrometer Anal. Chem. 2004, 76 (17) 5136-42
    • (2004) Anal. Chem. , vol.76 , Issue.17 , pp. 5136-5142
    • Salek, M.1    Costagliola, S.2    Lehmann, W.D.3
  • 21
    • 24044513966 scopus 로고    scopus 로고
    • Comparative evaluation of mass spectrometry platforms used in large-scale proteomics investigations
    • Elias, J. E.; Haas, W.; Faherty, B. K.; Gygi, S. P. Comparative evaluation of mass spectrometry platforms used in large-scale proteomics investigations Nat. Methods 2005, 2 (9) 667-75
    • (2005) Nat. Methods , vol.2 , Issue.9 , pp. 667-675
    • Elias, J.E.1    Haas, W.2    Faherty, B.K.3    Gygi, S.P.4
  • 22
    • 0023474127 scopus 로고
    • Proteolysis Data Bank: Specificity of α-chymotrypsin from computation of protein cleavages
    • Keil, B. Proteolysis Data Bank: specificity of α-chymotrypsin from computation of protein cleavages Protein Sequences Data Anal. 1987, 1 (1) 13-20
    • (1987) Protein Sequences Data Anal. , vol.1 , Issue.1 , pp. 13-20
    • Keil, B.1
  • 26
    • 0030729481 scopus 로고    scopus 로고
    • Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold
    • Perona, J. J.; Craik, C. S. Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold J. Biol. Chem. 1997, 272 (48) 29987-90
    • (1997) J. Biol. Chem. , vol.272 , Issue.48 , pp. 29987-29990
    • Perona, J.J.1    Craik, C.S.2
  • 27
    • 78549293591 scopus 로고    scopus 로고
    • Cleavage specificities of the brother and sister proteases Lys-C and Lys-N
    • Raijmakers, R.; Neerincx, P.; Mohammed, S.; Heck, A. J. Cleavage specificities of the brother and sister proteases Lys-C and Lys-N Chem. Commun. 2010, 46 (46) 8827-9
    • (2010) Chem. Commun. , vol.46 , Issue.46 , pp. 8827-8829
    • Raijmakers, R.1    Neerincx, P.2    Mohammed, S.3    Heck, A.J.4
  • 28
    • 33846632267 scopus 로고    scopus 로고
    • Prediction of missed cleavage sites in tryptic peptides aids protein identification in proteomics
    • Siepen, J. A.; Keevil, E. J.; Knight, D.; Hubbard, S. J. Prediction of missed cleavage sites in tryptic peptides aids protein identification in proteomics J. Proteome Res. 2007, 6 (1) 399-408
    • (2007) J. Proteome Res. , vol.6 , Issue.1 , pp. 399-408
    • Siepen, J.A.1    Keevil, E.J.2    Knight, D.3    Hubbard, S.J.4
  • 29
    • 1842864789 scopus 로고    scopus 로고
    • Enzymatic cleavage of proteins
    • Smith, B. J. Enzymatic cleavage of proteins Methods Mol. Biol. 2003, 211, 49-61
    • (2003) Methods Mol. Biol. , vol.211 , pp. 49-61
    • Smith, B.J.1
  • 30
    • 0017078893 scopus 로고
    • Tryptic hydrolysis at asparagine residues in globin chains
    • Casey, R.; Lang, A. Tryptic hydrolysis at asparagine residues in globin chains Biochim. Biophys. Acta 1976, 434 (1) 184-8
    • (1976) Biochim. Biophys. Acta , vol.434 , Issue.1 , pp. 184-188
    • Casey, R.1    Lang, A.2
  • 31
    • 0032262816 scopus 로고    scopus 로고
    • Influence of G187W/K188F/D189Y mutation in the substrate binding pocket of trypsin on β-casein processing
    • Chobert, J. M.; Briand, L.; Haertle, T. Influence of G187W/K188F/D189Y mutation in the substrate binding pocket of trypsin on β-casein processing J. Food Biochem. 1998, 22 (6) 529-545
    • (1998) J. Food Biochem. , vol.22 , Issue.6 , pp. 529-545
    • Chobert, J.M.1    Briand, L.2    Haertle, T.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.