An improved trypsin digestion method minimizes digestion-induced modifications on proteins

Analytical Biochemistry

Volumn 392, Issue 1, 2009, Pages 12-21

  Ren, Da ^a   Pipes, Gary D ^a   Liu, Dingjiang ^a   Shih, Liang Yu ^a   Nichols, Andrew C ^a   Treuheit, Michael J ^a   Brems, David N ^a   Bondarenko, Pavel V ^a  

^a AMGEN INC   (United States)

Author keywords

Deamidation; LC MS; N terminal glutamine cyclization; Peptide mapping; Posttranslational modification; Trypsin digestion

Indexed keywords

ALKALINITY; AMINO ACIDS; CHEMICAL REACTIONS; DRUG PRODUCTS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; MASS SPECTROMETRY; PEPTIDES;

DEAMIDATION; LC/MS; N-TERMINALS; PEPTIDE MAPPING; POST-TRANSLATIONAL MODIFICATIONS; TRYPSIN DIGESTION;

CYCLIZATION;

GLUTAMINE; GUANIDINE; IMMUNOGLOBULIN; TRYPSIN;

ALKYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; DEAMINATION; INTERMETHOD COMPARISON; PEPTIDE MAPPING; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; PROTEIN MODIFICATION; REACTION TIME; REDUCTION KINETICS; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; TIME OF FLIGHT MASS SPECTROMETRY;

EID: 67649311402     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.05.018     Document Type: Article

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