Addressing trypsin bias in large scale (Phospho)proteome analysis by size exclusion chromatography and secondary digestion of large post-trypsin peptides

Journal of Proteome Research

Volumn 10, Issue 2, 2011, Pages 800-811

  Tran, Bao Quoc ^a   Hernandez, Celine ^a,b   Waridel, Patrice ^a   Potts, Alexandra ^a   Barblan, Jachen ^a   Lisacek, Frederique ^b   Quadroni, Manfredo ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b SWISS INSTITUTE OF BIOINFORMATICS   (Switzerland)

Author keywords

Bottom up proteomics; Orthogonal digestion; Phosphorylation; Proteolysis; Sequence coverage; Size exclusion chromatography; Trypsin

Indexed keywords

PHOSPHOPROTEOME; PROTEOME; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CELL LYSATE; COMPUTER MODEL; FUNGUS CULTURE; GEL PERMEATION CHROMATOGRAPHY; HUMAN; MASS SPECTROMETRY; MELANOMA CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; PROTEOMICS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

CELL LINE, TUMOR; CHROMATOGRAPHY, GEL; COMPUTER SIMULATION; HUMANS; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PHOSPHOPROTEINS; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE ANALYSIS, PROTEIN; TRYPSIN;

HOMO SAPIENS; MAMMALIA; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 79955417151     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr100951t     Document Type: Article

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