Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations

Enzyme and Microbial Technology

Volumn 21, Issue 4, 1997, Pages 277-283

  Chiruvolu, Vijay ^a   Cregg, James M ^b   Meagher, Michael M ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

galactosidase; Aox1 promoter; Fed batch fermentation; Pichia pastoris; Recombinant protein

Indexed keywords

BIOSYNTHESIS; CONTINUOUS CELL CULTURE; ENZYMES; ETHANOL; FERMENTATION; GENES; GENETIC ENGINEERING; GLYCEROL; GROWTH KINETICS; METHANOL;

ALCOHOL OXIDASE; GALACTOSIDASE; PICHIA PASTORIS;

YEAST;

ALCOHOL; ALCOHOL OXIDASE; BETA GALACTOSIDASE; CARBON; GLYCEROL; RECOMBINANT ENZYME; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG MANUFACTURE; ENDOMYCETALES; ENZYME ACTIVITY; ENZYME SYNTHESIS; FERMENTATION; GENE FUSION; GENE REPRESSION; NONHUMAN; PROMOTER REGION; PROTEIN SYNTHESIS; YEAST;

EID: 0031239815     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(97)00042-2     Document Type: Article

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