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Volumn 21, Issue 6, 2013, Pages 423-434

Nucleic acid aptamers: New methods for selection, stabilization, and application in biomedical science

Author keywords

Aptamer; Drug delivery system; Imaging; Modification; SELEX; Stabilization

Indexed keywords

ADENOSINE TRIPHOSPHATE; ANTIBODY; APTAMER; CD137 ANTIGEN; DOXORUBICIN; GOLD NANOPARTICLE; MEMBRANE ANTIGEN; PEGAPTANIB; PHOTOSENSITIZING AGENT; SMALL INTERFERING RNA; THROMBIN; TOXIN; VASCULOTROPIN; VIRUS PROTEIN;

EID: 84888789869     PISSN: 19769148     EISSN: 20054483     Source Type: Journal    
DOI: 10.4062/biomolther.2013.085     Document Type: Review
Times cited : (170)

References (118)
  • 1
    • 0033899547 scopus 로고    scopus 로고
    • Towards the selection of phosphorothioate aptamers:Optimizing in vitro selection steps with phosphorothioate nucleotides
    • Andreola, M. L., Calmels, C., Michel, J., Toulmé, J. J. and Litvak, S. (2000) Towards the selection of phosphorothioate aptamers:optimizing in vitro selection steps with phosphorothioate nucleotides. Eur. J. Bio-chem. 267, 5032-5040.
    • (2000) Eur. J. Bio-chem , vol.267 , pp. 5032-5040
    • Andreola, M.L.1    Calmels, C.2    Michel, J.3    Toulmé, J.J.4    Litvak, S.5
  • 3
    • 33845723241 scopus 로고    scopus 로고
    • An aptamer-doxorubicin physical conjugate as a novel targeted drug-delivery platform
    • Bagalkot, V., Farokhzad, O. C., Langer, R. and Jon, S. (2006) An aptamer-doxorubicin physical conjugate as a novel targeted drug-delivery platform. Angew. Chem. Int. Ed. Engl. 45, 8149-8152.
    • (2006) Angew. Chem. Int. Ed. Engl , vol.45 , pp. 8149-8152
    • Bagalkot, V.1    Farokhzad, O.C.2    Langer, R.3    Jon, S.4
  • 4
    • 84888805558 scopus 로고    scopus 로고
    • Vemurafenib and ipilim-umab: New agents for metastatic melanoma
    • Banaszynski, M. and Kolesar, J. M. (2013) Vemurafenib and ipilim-umab: New agents for metastatic melanoma. Am. J. Health Syst. Pharm. 70, 1205-1210.
    • (2013) Am. J. Health Syst. Pharm , vol.70 , pp. 1205-1210
    • Banaszynski, M.1    Kolesar, J.M.2
  • 6
    • 14244263708 scopus 로고    scopus 로고
    • Nonequilibrium capillary electrophoresis of equilibrium mixtures: A universal tool for development of aptamers
    • Berezovski, M., Drabovich, A., Krylova, S. M., Musheev, M., Okhonin, V., Petrov, A. and Krylov, S. N. (2005) Nonequilibrium capillary electrophoresis of equilibrium mixtures: a universal tool for development of aptamers. J. Am. Chem. Soc. 127, 3165-3171.
    • (2005) J. Am. Chem. Soc , vol.127 , pp. 3165-3171
    • Berezovski, M.1    Drabovich, A.2    Krylova, S.M.3    Musheev, M.4    Okhonin, V.5    Petrov, A.6    Krylov, S.N.7
  • 8
    • 0035844150 scopus 로고    scopus 로고
    • Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. selective targeting of endothelial regulatory protein pigpen
    • Blank, M., Weinschenk, T., Priemer, M. and Schluesener, H. (2001) Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. selective targeting of endothelial regulatory protein pigpen. J. Biol. Chem. 276, 16464-16468.
    • (2001) J. Biol. Chem , vol.276 , pp. 16464-16468
    • Blank, M.1    Weinschenk, T.2    Priemer, M.3    Schluesener, H.4
  • 9
    • 51649114079 scopus 로고    scopus 로고
    • Competitive FRET-aptamer-based detection of methylphos- phonic acid, a common nerve agent metabolite
    • Bruno, J. G., Carrillo, M. P., Phillips, T., Vail, N. K. and Hanson, D. (2008) Competitive FRET-aptamer-based detection of methylphos- phonic acid, a common nerve agent metabolite. J. Fluoresc. 18, 867-876.
    • (2008) J. Fluoresc , vol.18 , pp. 867-876
    • Bruno, J.G.1    Carrillo, M.P.2    Phillips, T.3    Vail, N.K.4    Hanson, D.5
  • 10
    • 36048993546 scopus 로고    scopus 로고
    • Nanoplatforms for targeted molecular imaging in living subjects
    • Cai, W. and Chen, X. (2007) Nanoplatforms for targeted molecular imaging in living subjects. Small 3, 1840-1854.
    • (2007) Small , vol.3 , pp. 1840-1854
    • Cai, W.1    Chen, X.2
  • 11
    • 77949503400 scopus 로고    scopus 로고
    • CS-SELEX generates high-affi nity ssDNA aptamers as molecular probes for hepatitis C virus envelope glycoprotein E2
    • Chen, F., Hu, Y., Li, D., Chen, H. and Zhang, X. L. (2009) CS-SELEX generates high-affi nity ssDNA aptamers as molecular probes for hepatitis C virus envelope glycoprotein E2. PLoS One 4, e8142
    • (2009) PLoS One , vol.4
    • Chen, F.1    Hu, Y.2    Li, D.3    Chen, H.4    Zhang, X.L.5
  • 15
    • 80052198047 scopus 로고    scopus 로고
    • Quantum dot-aptamer nanoprobes for recognizing and labeling infl uenza A virus particles
    • Cui, Z. Q., Ren, Q., Wei, H. P., Chen, Z., Deng, J. Y., Zhang, Z. P. and Zhang, X. E. (2011) Quantum dot-aptamer nanoprobes for recognizing and labeling infl uenza A virus particles. Nanoscale. 3, 2454-2457.
    • (2011) Nanoscale , vol.3 , pp. 2454-2457
    • Cui, Z.Q.1    Ren, Q.2    Wei, H.P.3    Chen, Z.4    Deng, J.Y.5    Zhang, Z.P.6    Zhang, X.E.7
  • 17
    • 46849111987 scopus 로고    scopus 로고
    • Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer
    • Dollins, C. M., Nair, S., Boczkowski, D., Lee, J., Layzer, J. M., Gilboa, E. and Sullenger, B. A. (2008) Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer. Chem. Biol. 15, 675-682.
    • (2008) Chem. Biol , vol.15 , pp. 675-682
    • Dollins, C.M.1    Nair, S.2    Boczkowski, D.3    Lee, J.4    Layzer, J.M.5    Gilboa, E.6    Sullenger, B.A.7
  • 18
    • 0025074907 scopus 로고
    • In vitro selection of RNA molecules that bind specific ligands
    • Ellington, A. D. and Szostak, J. W. (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818-822.
    • (1990) Nature , vol.346 , pp. 818-822
    • Ellington, A.D.1    Szostak, J.W.2
  • 19
    • 0036959644 scopus 로고    scopus 로고
    • Preclinical and phase 1A clinical evaluation of an anti-vegf pegylated aptamer (Eye001) for the treatment of exudative age-related macular degeneration
    • Eyetech Study Group
    • Eyetech Study Group. (2002) Preclinical and phase 1A clinical evaluation of an anti-vegf pegylated aptamer (Eye001) for the treatment of exudative age-related macular degeneration. Retina. 22, 143-152.
    • (2002) Retina , vol.22 , pp. 143-152
  • 20
    • 0032896149 scopus 로고    scopus 로고
    • Novel approach to specific growth factor inhibition in vivo: Antagonism of platelet-derived growth factor in glomerulonephritis by aptamers
    • Floege, J., Ostendorf, T., Janssen, U., Burg, M., Radeke, H. H., Varg-eese, C., Gill, S. C., Green, L. S. and Janjić, N. (1999) Novel approach to specific growth factor inhibition in vivo: antagonism of platelet-derived growth factor in glomerulonephritis by aptamers. Am. J. Pathol. 154, 169-179.
    • (1999) Am. J. Pathol , vol.154 , pp. 169-179
    • Floege, J.1    Ostendorf, T.2    Janssen, U.3    Burg, M.4    Radeke, H.H.5    Varg-Eese, C.6    Gill, S.C.7    Green, L.S.8    Janjić, N.9
  • 21
    • 36749019706 scopus 로고    scopus 로고
    • Local tolerance and systemic safety of pegaptanib sodium in the dog and rabbit
    • Foy, J. W., Rittenhouse, K., Modi, M. and Patel, M. (2007) Local tolerance and systemic safety of pegaptanib sodium in the dog and rabbit. J. Ocul. Pharmacol. Ther. 23, 452-466.
    • (2007) J. Ocul. Pharmacol. Ther , vol.23 , pp. 452-466
    • Foy, J.W.1    Rittenhouse, K.2    Modi, M.3    Patel, M.4
  • 22
    • 84874912010 scopus 로고    scopus 로고
    • Use of oligonucle-otide aptamer ligands to modulate the function of immune receptors
    • Gilboa, E., McNamara, J. 2nd and Pastor, F. (2013) Use of oligonucle-otide aptamer ligands to modulate the function of immune receptors. Clin. Cancer Res. 19, 1054-1062.
    • (2013) Clin. Cancer Res , vol.19 , pp. 1054-1062
    • Gilboa, E.1    McNamara II, J.2    Pastor, F.3
  • 23
    • 84870238164 scopus 로고    scopus 로고
    • Effect of BAX499 aptamer on tissue factor pathway inhibitor function and thrombin generation in models of hemophilia
    • Gissel, M., Orfeo, T., Foley, J. H. and Butenas, S. (2012) Effect of BAX499 aptamer on tissue factor pathway inhibitor function and thrombin generation in models of hemophilia. Thromb. Res. 130, 948-955.
    • (2012) Thromb. Res , vol.130 , pp. 948-955
    • Gissel, M.1    Orfeo, T.2    Foley, J.H.3    Butenas, S.4
  • 25
    • 79956096847 scopus 로고    scopus 로고
    • DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes
    • Hamula, C. L., Le, X. C. and Li, X. F. (2011) DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Anal. Chem. 83, 3640-3647.
    • (2011) Anal. Chem , vol.83 , pp. 3640-3647
    • Hamula, C.L.1    Le, X.C.2    Li, X.F.3
  • 26
    • 84875636510 scopus 로고    scopus 로고
    • Engineering a cell-surface aptamer circuit for targeted and amplifi ed photodynamic cancer therapy
    • Han, D., Zhu, G., Wu, C., Zhu, Z., Chen, T., Zhang, X. and Tan, W. (2013) Engineering a cell-surface aptamer circuit for targeted and amplifi ed photodynamic cancer therapy. ACS Nano 7, 2312-2319.
    • (2013) ACS Nano , vol.7 , pp. 2312-2319
    • Han, D.1    Zhu, G.2    Wu, C.3    Zhu, Z.4    Chen, T.5    Zhang, X.6    Tan, W.7
  • 29
    • 79960231780 scopus 로고    scopus 로고
    • Intra-accumbens injection of a dopamine aptamer abates MK-801-induced cognitive dysfunction in a model of schizophrenia
    • Holahan, M. R., Madularu, D., McConnell, E. M., Walsh, R. and DeRosa, M. C. (2011) Intra-accumbens injection of a dopamine aptamer abates MK-801-induced cognitive dysfunction in a model of schizophrenia. PLoS One 6, e22239.
    • (2011) PLoS One , vol.6
    • Holahan, M.R.1    Madularu, D.2    McConnell, E.M.3    Walsh, R.4    Derosa, M.C.5
  • 30
    • 0034602510 scopus 로고    scopus 로고
    • Effect of oligodeoxynucleotide thrombin aptamer on thrombin inhibition by heparin cofactor II and antithrombin
    • Holland, C. A., Henry, A. T., Whinna, H. C. and Church, F. C. (2000) Effect of oligodeoxynucleotide thrombin aptamer on thrombin inhibition by heparin cofactor II and antithrombin. FEBS Lett. 484, 87-91.
    • (2000) FEBS Lett , vol.484 , pp. 87-91
    • Holland, C.A.1    Henry, A.T.2    Whinna, H.C.3    Church, F.C.4
  • 31
    • 80052257765 scopus 로고    scopus 로고
    • Molecular imaging with nucleic acid aptamers
    • Hong, H., Goel, S., Zhang, Y. and Cai, W. (2011) Molecular imaging with nucleic acid aptamers. Curr. Med. Chem. 18, 4195-4205.
    • (2011) Curr. Med. Chem , vol.18 , pp. 4195-4205
    • Hong, H.1    Goel, S.2    Zhang, Y.3    Cai, W.4
  • 32
    • 0026022298 scopus 로고
    • Muromonab CD-3: A review of its pharmacology, pharmacokinetics, and clinical use in transplantation
    • Hooks, M. A., Wade, C. S. and Millikan, W. J. (1991) Muromonab CD-3: a review of its pharmacology, pharmacokinetics, and clinical use in transplantation. Pharmacotherapy 11, 26-37.
    • (1991) Pharmacotherapy , vol.11 , pp. 26-37
    • Hooks, M.A.1    Wade, C.S.2    Millikan, W.J.3
  • 34
    • 65549102827 scopus 로고    scopus 로고
    • Molecular assembly of an aptamer-drug conjugate for targeted drug delivery to tumor cells
    • Huang, Y. F., Shangguan, D., Liu, H., Phillips, J. A., Zhang, X., Chen, Y. and Tan, W. (2009) Molecular assembly of an aptamer-drug conjugate for targeted drug delivery to tumor cells. Chembiochem. 10, 862-868.
    • (2009) Chembiochem , vol.10 , pp. 862-868
    • Huang, Y.F.1    Shangguan, D.2    Liu, H.3    Phillips, J.A.4    Zhang, X.5    Chen, Y.6    Tan, W.7
  • 35
    • 84878226716 scopus 로고    scopus 로고
    • An aptamer-siRNA chimera silences the eukaryotic elongation factor 2 gene and induces apoptosis in cancers expressing αvβ3 integrin
    • Hussain, A. F., Tur, M. K. and Barth, S. (2013) An aptamer-siRNA chimera silences the eukaryotic elongation factor 2 gene and induces apoptosis in cancers expressing αvβ3 integrin. Nucleic Acid Ther. 23, 203-212.
    • (2013) Nucleic Acid Ther , vol.23 , pp. 203-212
    • Hussain, A.F.1    Tur, M.K.2    Barth, S.3
  • 36
    • 75149193491 scopus 로고    scopus 로고
    • A nucleolin-targeted multimodal nanoparticle imaging probe for tracking cancer cells using an ap-tamer
    • Hwang do, W., Ko, H. Y., Lee, J. H., Kang, H., Ryu, S. H., Song, I. C., Lee, D. S. and Kim, S. (2010) A nucleolin-targeted multimodal nanoparticle imaging probe for tracking cancer cells using an ap-tamer. J. Nucl. Med. 51, 98-105.
    • (2010) J. Nucl. Med , vol.51 , pp. 98-105
    • Hwang Do, W.1    Ko, H.Y.2    Lee, J.H.3    Kang, H.4    Ryu, S.H.5    Song, I.C.6    Lee, D.S.7    Kim, S.8
  • 39
    • 0032860385 scopus 로고    scopus 로고
    • Aptamers: An emerging class of molecules that rival antibodies in diagnostics
    • Jayasena, S. D. (1999) Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin. Chem. 45, 1628-1650.
    • (1999) Clin. Chem , vol.45 , pp. 1628-1650
    • Jayasena, S.D.1
  • 40
    • 0028224199 scopus 로고
    • High-resolution molecular discrimination by RNA
    • Jenison, R. D., Gill, S. C., Pardi, A. and Polisky, B. (1994) High-resolution molecular discrimination by RNA. Science 263, 1425-1429.
    • (1994) Science , vol.263 , pp. 1425-1429
    • Jenison, R.D.1    Gill, S.C.2    Pardi, A.3    Polisky, B.4
  • 41
    • 84867935571 scopus 로고    scopus 로고
    • Artificial specific binders directly recovered from chemically modified nucleic Acid libraries
    • Kasahara, Y. and Kuwahara, M. (2012) Artificial specific binders directly recovered from chemically modified nucleic Acid libraries. J. Nucleic Acids 2012, 156482.
    • (2012) J. Nucleic Acids 2012 , pp. 156482
    • Kasahara, Y.1    Kuwahara, M.2
  • 42
    • 84873727247 scopus 로고    scopus 로고
    • 2',4'-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2'-O, 4'-C-methylene-bridged/linked bicyclic ri-bonucleotides
    • Kasahara, Y., Irisawa, Y., Ozaki, H., Obika, S. and Kuwahara, M. (2013) 2',4'-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2'-O, 4'-C-methylene-bridged/linked bicyclic ri-bonucleotides. Bioorg. Med. Chem. Lett. 23, 1288-1292.
    • (2013) Bioorg. Med. Chem. Lett , vol.23 , pp. 1288-1292
    • Kasahara, Y.1    Irisawa, Y.2    Ozaki, H.3    Obika, S.4    Kuwahara, M.5
  • 44
    • 22444448397 scopus 로고    scopus 로고
    • Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization
    • Kempeneers, V., Renders, M., Froeyen, M. and Herdewijn, P. (2005) Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization. Nucleic Acids Res. 33, 3828-3836.
    • (2005) Nucleic Acids Res , vol.33 , pp. 3828-3836
    • Kempeneers, V.1    Renders, M.2    Froeyen, M.3    Herdewijn, P.4
  • 45
    • 77955542791 scopus 로고    scopus 로고
    • A drug-loaded aptamer-gold nanoparticle bioconjugate for combined CT imaging and therapy of prostate cancer
    • Kim, D., Jeong, Y. Y. and Jon, S. (2010) A drug-loaded aptamer-gold nanoparticle bioconjugate for combined CT imaging and therapy of prostate cancer. ACS Nano 4, 3689-3696.
    • (2010) ACS Nano , vol.4 , pp. 3689-3696
    • Kim, D.1    Jeong, Y.Y.2    Jon, S.3
  • 46
    • 33750725110 scopus 로고    scopus 로고
    • Improved sensitivity and physical properties of sol-gel protein chips using large-scale material screening and selection
    • Kim, S., Kim, Y., Kim, P., Ha, J., Kim, K., Sohn, M., Yoo, J. S., Lee, J., Kwon, J. A. and Lee, K. N. (2006) Improved sensitivity and physical properties of sol-gel protein chips using large-scale material screening and selection. Anal. Chem. 78, 7392-7396.
    • (2006) Anal. Chem , vol.78 , pp. 7392-7396
    • Kim, S.1    Kim, Y.2    Kim, P.3    Ha, J.4    Kim, K.5    Sohn, M.6    Yoo, J.S.7    Lee, J.8    Kwon, J.A.9    Lee, K.N.10
  • 50
    • 48349115007 scopus 로고    scopus 로고
    • Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides
    • Kuwahara, M., Obika, S., Nagashima, J., Ohta, Y., Suto, Y., Ozaki, H., Sawai, H. and Imanishi, T. (2008) Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides. Nucleic Acids Res. 36, 4257-4265.
    • (2008) Nucleic Acids Res , vol.36 , pp. 4257-4265
    • Kuwahara, M.1    Obika, S.2    Nagashima, J.3    Ohta, Y.4    Suto, Y.5    Ozaki, H.6    Sawai, H.7    Imanishi, T.8
  • 51
    • 0141545111 scopus 로고    scopus 로고
    • Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA poly-merases during PCR
    • Kuwahara, M., Takahata, Y., Shoji, A., Ozaki, A. N., Ozaki, H. and Sawai, H. (2003) Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA poly-merases during PCR. Bioorg. Med. Chem. Lett. 13, 3735-3738.
    • (2003) Bioorg. Med. Chem. Lett , vol.13 , pp. 3735-3738
    • Kuwahara, M.1    Takahata, Y.2    Shoji, A.3    Ozaki, A.N.4    Ozaki, H.5    Sawai, H.6
  • 52
    • 78649483109 scopus 로고    scopus 로고
    • Study on suitability of KOD dna polymerase for enzymatic production of artificial nucleic acids using base/sugar modified nucleoside triphosphates
    • Kuwahara, M., Takano, Y., Kasahara, Y., Nara, H., Ozaki, H., Sawai, H., Sugiyama, A. and Obika, S. (2010) Study on suitability of KOD dna polymerase for enzymatic production of artificial nucleic acids using base/sugar modified nucleoside triphosphates. Molecules 15, 8229-8240.
    • (2010) Molecules , vol.15 , pp. 8229-8240
    • Kuwahara, M.1    Takano, Y.2    Kasahara, Y.3    Nara, H.4    Ozaki, H.5    Sawai, H.6    Sugiyama, A.7    Obika, S.8
  • 53
    • 84920506277 scopus 로고    scopus 로고
    • Transcription and reverse transcription of ar-tificial nucleic acids involving backbone modification by template directed DNA polymerase reactions. Bioorg. Med
    • Kuwahara, M., Takeshima, H., Nagashima, J., Minezaki, S., Ozaki, H. and Sawai, H. (2009) Transcription and reverse transcription of ar-tificial nucleic acids involving backbone modification by template directed DNA polymerase reactions. Bioorg. Med. Chem. 17, 3782-3788.
    • (2009) Chem , vol.17 , pp. 3782-3788
    • Kuwahara, M.1    Takeshima, H.2    Nagashima, J.3    Minezaki, S.4    Ozaki, H.5    Sawai, H.6
  • 54
    • 0028136665 scopus 로고
    • The application of a modified nucleotide in aptamer selection: Novel thrombin aptamers containing 5-(1-pentynyl)-2'-deoxyuridine
    • Latham, J. A., Johnson, R. and Toole, J. J. (1994) The application of a modified nucleotide in aptamer selection: novel thrombin aptamers containing 5-(1-pentynyl)-2'-deoxyuridine. Nucleic Acids Res. 22, 2817-2822.
    • (1994) Nucleic Acids Res , vol.22 , pp. 2817-2822
    • Latham, J.A.1    Johnson, R.2    Toole, J.J.3
  • 56
    • 0029138009 scopus 로고
    • RNA aptamers that bind fl avin and nicotinamide redox cofactors
    • Lauhon, C. T. and Szostak, J. W. (1995) RNA aptamers that bind fl avin and nicotinamide redox cofactors. J. Am. Chem. Soc. 117, 1246-1257.
    • (1995) J. Am. Chem. Soc , vol.117 , pp. 1246-1257
    • Lauhon, C.T.1    Szostak, J.W.2
  • 57
    • 33750204643 scopus 로고    scopus 로고
    • Dynamic assembly of primers on nucleic acid templates
    • Leal, N. A., Sukeda, M. and Benner, S. A. (2006) Dynamic assembly of primers on nucleic acid templates. Nucleic Acids Res. 34, 4702-4710.
    • (2006) Nucleic Acids Res , vol.34 , pp. 4702-4710
    • Leal, N.A.1    Sukeda, M.2    Benner, S.A.3
  • 59
    • 84870770430 scopus 로고    scopus 로고
    • Selective and sensitive turn-on detection of adenosine triphosphate and thrombin based on bi-functional fluorescent oligonucleotide probe
    • Li, F., Du, Z., Yang, L. and Tang, B. (2013) Selective and sensitive turn-on detection of adenosine triphosphate and thrombin based on bi-functional fluorescent oligonucleotide probe. Biosens. Bioelectron. 41, 907-910.
    • (2013) Biosens. Bioelectron , vol.41 , pp. 907-910
    • Li, F.1    Du, Z.2    Yang, L.3    Tang, B.4
  • 60
    • 52449123115 scopus 로고    scopus 로고
    • Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety
    • Li, M., Lin, N., Huang, Z., Du, L., Altier, C., Fang, H. and Wang, B. (2008) Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety. J. Am. Chem. Soc. 130, 12636-12638.
    • (2008) J. Am. Chem. Soc , vol.130 , pp. 12636-12638
    • Li, M.1    Lin, N.2    Huang, Z.3    Du, L.4    Altier, C.5    Fang, H.6    Wang, B.7
  • 61
    • 84867754001 scopus 로고    scopus 로고
    • Novel HER2 aptamer selectively delivers cyto-toxic drug to HER2-positive breast cancer cells in vitro
    • Liu, Z., Duan, J. H., Song, Y. M., Ma, J., Wang, F. D., Lu, X., Yang X. D. (2012) Novel HER2 aptamer selectively delivers cyto-toxic drug to HER2-positive breast cancer cells in vitro. J. Transl. Med. 10, 148.
    • (2012) J. Transl. Med , vol.10 , pp. 148
    • Liu, Z.1    Duan, J.H.2    Song, Y.M.3    Ma, J.4    Wang, F.D.5    Lu, X.6    Yang, X.D.7
  • 65
    • 77958606867 scopus 로고    scopus 로고
    • Optimization of ultrasound contrast agents with computational models to improve selection of ligands and binding strength
    • Maul, T. M., Dudgeon, D. D., Beste, M. T., Hammer, D. A., Lazo, J. S., Villanueva, F. S. and Wagner, W. R. (2010) Optimization of ultrasound contrast agents with computational models to improve selection of ligands and binding strength. Biotechnol. Bioeng. 107, 854-864.
    • (2010) Biotechnol. Bioeng , vol.107 , pp. 854-864
    • Maul, T.M.1    Dudgeon, D.D.2    Beste, M.T.3    Hammer, D.A.4    Lazo, J.S.5    Villanueva, F.S.6    Wagner, W.R.7
  • 66
  • 67
    • 0344874727 scopus 로고    scopus 로고
    • Molecular imaging of pro-teolytic activity in cancer
    • McIntyre, J. O. and Matrisian, L. M. (2003) Molecular imaging of pro-teolytic activity in cancer. J. Cell. Biochem. 90, 1087-1097.
    • (2003) J. Cell. Biochem , vol.90 , pp. 1087-1097
    • McIntyre, J.O.1    Matrisian, L.M.2
  • 70
    • 84865416584 scopus 로고    scopus 로고
    • Targeted delivery of chemotherapy agents using a liver can-cer-specific aptamer
    • Meng, L., Yang, L., Zhao, X., Zhang, L., Zhu, H., Liu, C. and Tan, W. (2012) Targeted delivery of chemotherapy agents using a liver can-cer-specific aptamer. PLoS One 7, e33434.
    • (2012) PLoS One , vol.7
    • Meng, L.1    Yang, L.2    Zhao, X.3    Zhang, L.4    Zhu, H.5    Liu, C.6    Tan, W.7
  • 72
    • 26444463933 scopus 로고    scopus 로고
    • Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase
    • Mosing, R. K., Mendonsa, S. D. and Bowser, M. T. (2005) Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase. Anal. Chem. 77, 6107-6112.
    • (2005) Anal. Chem , vol.77 , pp. 6107-6112
    • Mosing, R.K.1    Mendonsa, S.D.2    Bowser, M.T.3
  • 75
    • 0029758370 scopus 로고    scopus 로고
    • Mirror-design of L-oligonucleotide ligands binding to L-argi-nine
    • Nolte, A., Klussmann, S., Bald, R., Erdmann, V. A. and Furste, J. P. (1996) Mirror-design of L-oligonucleotide ligands binding to L-argi-nine. Nat. Biotechnol. 14, 1116-1119.
    • (1996) Nat. Biotechnol , vol.14 , pp. 1116-1119
    • Nolte, A.1    Klussmann, S.2    Bald, R.3    Erdmann, V.A.4    Furste, J.P.5
  • 76
    • 77953672623 scopus 로고    scopus 로고
    • Denosumab
    • Pageau, S. C. (2009) Denosumab. MAbs 1, 210-215.
    • (2009) MAbs , vol.1 , pp. 210-215
    • Pageau, S.C.1
  • 77
    • 65349091927 scopus 로고    scopus 로고
    • Selection and elution of aptamers using nano-porous sol-gel arrays with integrated microheaters
    • Park, S. M., Ahn, J. Y., Jo, M., Lee, D. K., Lis, J. T., Craighead, H. G. and Kim, S. (2009) Selection and elution of aptamers using nano-porous sol-gel arrays with integrated microheaters. Lab Chip 9, 1206-1212.
    • (2009) Lab Chip , vol.9 , pp. 1206-1212
    • Park, S.M.1    Ahn, J.Y.2    Jo, M.3    Lee, D.K.4    Lis, J.T.5    Craighead, H.G.6    Kim, S.7
  • 79
    • 79959516872 scopus 로고    scopus 로고
    • Synthesis and radio-labeling of chelator-RNA aptamer bioconjugates with copper-64 for targeted molecular imaging
    • Rockey, W. M., Huang, L., Kloepping, K. C., Baumhover, N. J., Giangrande, P. H. and Schultz, M. K. (2011) Synthesis and radio-labeling of chelator-RNA aptamer bioconjugates with copper-64 for targeted molecular imaging. Bioorg. Med. Chem. 19, 4080-4090.
    • (2011) Bioorg. Med. Chem , vol.19 , pp. 4080-4090
    • Rockey, W.M.1    Huang, L.2    Kloepping, K.C.3    Baumhover, N.J.4    Giangrande, P.H.5    Schultz, M.K.6
  • 80
    • 84255160968 scopus 로고    scopus 로고
    • Preculture of PBMCs at high cell density increases sensitivity of T-cell responses, revealing cytokine release by CD28 superagonist TGN1412
    • Römer, P. S., Berr, S., Avota, E., Na, S. Y., Battaglia, M., ten Berge, I., Einsele, H. and Hünig, T. (2011) Preculture of PBMCs at high cell density increases sensitivity of T-cell responses, revealing cytokine release by CD28 superagonist TGN1412. Blood 118, 6772-6782.
    • (2011) Blood , vol.118 , pp. 6772-6782
    • Römer, P.S.1    Berr, S.2    Avota, E.3    Na, S.Y.4    Battaglia, M.5    Ten Berge, I.6    Einsele, H.7    Hünig, T.8
  • 81
    • 0032493654 scopus 로고    scopus 로고
    • 2'-Fluo-ropyrimidine RNA-based aptamers to the 165-amino acid form of vascular endothelial growth factor (VEGF165). Inhibition of receptor binding and VEGF-induced vascular permeability through interactions requiring the exon 7-encoded domain
    • Ruckman, J., Green, L. S., Beeson, J., Waugh, S., Gillette, W. L., Hen-ninger, D. D., Claesson-Welsh, L. and Janjić, N. (1998) 2'-Fluo-ropyrimidine RNA-based aptamers to the 165-amino acid form of vascular endothelial growth factor (VEGF165). Inhibition of receptor binding and VEGF-induced vascular permeability through interactions requiring the exon 7-encoded domain. J. Biol. Chem. 273, 20556-20567.
    • (1998) J. Biol. Chem , vol.273 , pp. 20556-20567
    • Ruckman, J.1    Green, L.S.2    Beeson, J.3    Waugh, S.4    Gillette, W.L.5    Hen-Ninger, D.D.6    Claesson-Welsh, L.7    Janjić, N.8
  • 82
    • 79960078032 scopus 로고    scopus 로고
    • Tumor targeted quantum dot-mucin 1 aptamer-doxorubicin conjugate for imaging and treatment of cancer
    • Savla, R., Taratula, O., Garbuzenko, O. and Minko, T. (2011) Tumor targeted quantum dot-mucin 1 aptamer-doxorubicin conjugate for imaging and treatment of cancer. J. Control. Release 153, 16-22.
    • (2011) J. Control. Release , vol.153 , pp. 16-22
    • Savla, R.1    Taratula, O.2    Garbuzenko, O.3    Minko, T.4
  • 84
    • 0029165745 scopus 로고
    • High-affinity ssDNA inhibitors of the reverse transcriptase of type 1 human immunodeficiency virus
    • Schneider, D. J., Feigon, J., Hostomsky, Z. and Gold, L. (1995) High-affinity ssDNA inhibitors of the reverse transcriptase of type 1 human immunodeficiency virus. Biochemistry 34, 9599-9610.
    • (1995) Biochemistry , vol.34 , pp. 9599-9610
    • Schneider, D.J.1    Feigon, J.2    Hostomsky, Z.3    Gold, L.4
  • 85
    • 77957556157 scopus 로고    scopus 로고
    • In vivo fluorescence imaging of tumors using molecular aptamers generated by cell-SELEX
    • Shi, H., Tang, Z., Kim, Y., Nie, H., Huang, Y. F., He, X., Deng, K., Wang, K. and Tan, W. (2010) In vivo fluorescence imaging of tumors using molecular aptamers generated by cell-SELEX. Chem. Asian J. 5, 2209-2213.
    • (2010) Chem. Asian J , vol.5 , pp. 2209-2213
    • Shi, H.1    Tang, Z.2    Kim, Y.3    Nie, H.4    Huang, Y.F.5    He, X.6    Deng, K.7    Wang, K.8    Tan, W.9
  • 86
    • 33846799193 scopus 로고    scopus 로고
    • Modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity
    • Shoji, A., Kuwahara, M., Ozaki, H. and Sawai, H. (2007) Modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity. J. Am. Chem. Soc. 129, 1456-1464.
    • (2007) J. Am. Chem. Soc , vol.129 , pp. 1456-1464
    • Shoji, A.1    Kuwahara, M.2    Ozaki, H.3    Sawai, H.4
  • 88
    • 84876264381 scopus 로고    scopus 로고
    • Selection of DNA aptamers against epithelial cell adhesion molecule for cancer cell imaging and circulating tumor cell capture
    • Song, Y., Zhu, Z., An, Y., Zhang, W., Zhang, H., Liu, D., Yu, C., Duan, W. and Yang, C. J. (2013) Selection of DNA aptamers against epithelial cell adhesion molecule for cancer cell imaging and circulating tumor cell capture. Anal. Chem. 85, 4141-4149.
    • (2013) Anal. Chem , vol.85 , pp. 4141-4149
    • Song, Y.1    Zhu, Z.2    An, Y.3    Zhang, W.4    Zhang, H.5    Liu, D.6    Yu, C.7    Duan, W.8    Yang, C.J.9
  • 89
    • 84870448531 scopus 로고    scopus 로고
    • Target-specific delivery of doxorubicin to retinoblastoma using epithelial cell adhesion molecule aptamer
    • Subramanian, N., Raghunathan, V., Kanwar, J. R., Kanwar, R. K., El-churi, S. V., Khetan, V. and Krishnakumar, S. (2012) Target-specific delivery of doxorubicin to retinoblastoma using epithelial cell adhesion molecule aptamer. Mol. Vis. 18, 2783-2795.
    • (2012) Mol. Vis , vol.18 , pp. 2783-2795
    • Subramanian, N.1    Raghunathan, V.2    Kanwar, J.R.3    Kanwar, R.K.4    El-Churi, S.V.5    Khetan, V.6    Krishnakumar, S.7
  • 91
    • 79960919903 scopus 로고    scopus 로고
    • Pharmacokinetic characterization of an RNA aptamer against osteopontin and demonstration of in vivo efficacy in reversing growth of human breast cancer cells
    • Talbot, L. J., Mi, Z., Bhattacharya, S. D., Kim, V., Guo, H. and Kuo, P. C. (2011) Pharmacokinetic characterization of an RNA aptamer against osteopontin and demonstration of in vivo efficacy in reversing growth of human breast cancer cells. Surgery 150, 224-230.
    • (2011) Surgery , vol.150 , pp. 224-230
    • Talbot, L.J.1    Mi, Z.2    Bhattacharya, S.D.3    Kim, V.4    Guo, H.5    Kuo, P.C.6
  • 92
    • 64149094557 scopus 로고    scopus 로고
    • Generating aptamers for recognition of virus-infected cells
    • Tang, Z., Parekh, P., Turner, P., Moyer, R. W. and Tan, W. (2009) Generating aptamers for recognition of virus-infected cells. Clin. Chem. 55, 813-822.
    • (2009) Clin. Chem , vol.55 , pp. 813-822
    • Tang, Z.1    Parekh, P.2    Turner, P.3    Moyer, R.W.4    Tan, W.5
  • 93
    • 35548977605 scopus 로고    scopus 로고
    • Enzymatic synthesis of DNA on glycerol nucleic acid templates without stable duplex formation between product and template
    • Tsai, C. H., Chen, J. and Szostak, J. W. (2007) Enzymatic synthesis of DNA on glycerol nucleic acid templates without stable duplex formation between product and template. Proc. Natl. Acad. Sci. USA 104, 14598-14603.
    • (2007) Proc. Natl. Acad. Sci. USA , vol.104 , pp. 14598-14603
    • Tsai, C.H.1    Chen, J.2    Szostak, J.W.3
  • 94
    • 14544286521 scopus 로고    scopus 로고
    • Building and breeding molecules to spy on cells and tumors
    • Tsien, R. Y. (2005) Building and breeding molecules to spy on cells and tumors. FEBS Lett. 579, 927-932.
    • (2005) FEBS Lett , vol.579 , pp. 927-932
    • Tsien, R.Y.1
  • 95
    • 0025194307 scopus 로고
    • Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA poly-merase
    • Tuerk, C. and Gold, L. (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA poly-merase. Science 249, 505-510.
    • (1990) Science , vol.249 , pp. 505-510
    • Tuerk, C.1    Gold, L.2
  • 98
    • 67649329663 scopus 로고    scopus 로고
    • Efficient enzymatic synthesis of LNA-modifi ed DNA duplexes using KOD DNA poly-merase
    • Veedu, R. N., Vester, B. and Wengel, J. (2009) Efficient enzymatic synthesis of LNA-modifi ed DNA duplexes using KOD DNA poly-merase. Org. Biomol. Chem. 7, 1404-1409.
    • (2009) Org. Biomol. Chem , vol.7 , pp. 1404-1409
    • Veedu, R.N.1    Vester, B.2    Wengel, J.3
  • 99
    • 79958012143 scopus 로고    scopus 로고
    • Aptamer-conjugated nanobubbles for targeted ultrasound molecular imaging
    • Wang, C. H., Huang, Y. F. and Yeh, C. K. (2011) Aptamer-conjugated nanobubbles for targeted ultrasound molecular imaging. Langmuir 27, 6971-6976.
    • (2011) Langmuir , vol.27 , pp. 6971-6976
    • Wang, C.H.1    Huang, Y.F.2    Yeh, C.K.3
  • 100
    • 0033995256 scopus 로고    scopus 로고
    • In vitro selection of novel RNA ligands that bind human cytomegalovirus and block viral infection
    • Wang, J., Jiang, H. and Liu, F. (2000) In vitro selection of novel RNA ligands that bind human cytomegalovirus and block viral infection. RNA 6, 571-583.
    • (2000) RNA , vol.6 , pp. 571-583
    • Wang, J.1    Jiang, H.2    Liu, F.3
  • 103
  • 104
    • 55449127119 scopus 로고    scopus 로고
    • Cell-specific induction of apoptosis by rationally designed bivalent aptamer-siRNA transcripts silencing eukaryotic elongation factor 2
    • Wullner, U., Neef, I., Eller, A., Kleines, M., Tur, M. K. and Barth, S. (2008) Cell-specific induction of apoptosis by rationally designed bivalent aptamer-siRNA transcripts silencing eukaryotic elongation factor 2. Curr. Cancer Drug Targets 8, 554-565.
    • (2008) Curr. Cancer Drug Targets , vol.8 , pp. 554-565
    • Wullner, U.1    Neef, I.2    Eller, A.3    Kleines, M.4    Tur, M.K.5    Barth, S.6
  • 105
    • 79954451170 scopus 로고    scopus 로고
    • A smart magnetic resonance imaging contrast agent responsive to adenosine based on a DNA aptamer-conjugat-ed gadolinium complex
    • Xu, W. and Lu, Y. (2011) A smart magnetic resonance imaging contrast agent responsive to adenosine based on a DNA aptamer-conjugat-ed gadolinium complex. Chem. Commun. 47, 4998-5000.
    • (2011) Chem. Commun , vol.47 , pp. 4998-5000
    • Xu, W.1    Lu, Y.2
  • 106
    • 0033530825 scopus 로고    scopus 로고
    • Aptamers containing thymidine 3'-O-phosphorodithioates: Synthesis and binding to nuclear factor-κB
    • Yang, X., Fennewald, S., Luxon, B. A., Aronson, J., Herzog, N. K. and Gorenstein, D. G. (1999) Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-κB. Bioorg. Med. Chem. Lett. 9, 3357-3362.
    • (1999) Bioorg. Med. Chem. Lett , vol.9 , pp. 3357-3362
    • Yang, X.1    Fennewald, S.2    Luxon, B.A.3    Aronson, J.4    Herzog, N.K.5    Gorenstein, D.G.6
  • 107
    • 80053420613 scopus 로고    scopus 로고
    • An-giogenin-mediated photosensitizer-aptamer conjugate for photody-namic therapy
    • Yang, X., Huang, J., Wang, K., Li, W., Cui, L. and Li, X. (2011) An-giogenin-mediated photosensitizer-aptamer conjugate for photody-namic therapy. ChemMedChem 6, 1788-1780.
    • (2011) ChemMedChem , vol.6 , pp. 1780-1788
    • Yang, X.1    Huang, J.2    Wang, K.3    Li, W.4    Cui, L.5    Li, X.6
  • 108
    • 34948819141 scopus 로고    scopus 로고
    • Smart turn-on magnetic resonance contrast agents based on aptamer-functionalized superparamagnetic iron oxide nanoparticles
    • Yigit, M. V., Mazumdar, D., Kim, H. K., Lee, J. H., Odintsov, B. and Lu, Y. (2007) Smart turn-on magnetic resonance contrast agents based on aptamer-functionalized superparamagnetic iron oxide nanoparticles. Chembiochem 8, 1675-1678.
    • (2007) Chembiochem , vol.8 , pp. 1675-1678
    • Yigit, M.V.1    Mazumdar, D.2    Kim, H.K.3    Lee, J.H.4    Odintsov, B.5    Lu, Y.6
  • 110
    • 84869059101 scopus 로고    scopus 로고
    • Targeted quantum dots fluorescence probes functionalized with aptamer and peptide for transferrin receptor on tumor cells
    • Zhang, M. Z., Yu, R. N., Chen, J., Ma, Z. Y. and Zhao, Y. D. (2012) Targeted quantum dots fluorescence probes functionalized with aptamer and peptide for transferrin receptor on tumor cells. Nano-technology 23, 485104.
    • (2012) Nano-technology , vol.23 , pp. 485104
    • Zhang, M.Z.1    Yu, R.N.2    Chen, J.3    Ma, Z.Y.4    Zhao, Y.D.5
  • 111
    • 80052229081 scopus 로고    scopus 로고
    • Tumor-targeted drug delivery with aptamers
    • Zhang, Y., Hong, H. and Cai W. (2011) Tumor-targeted drug delivery with aptamers. Curr. Med. Chem. 18, 4185-4194.
    • (2011) Curr. Med. Chem , vol.18 , pp. 4185-4194
    • Zhang, Y.1    Hong, H.2    Cai, W.3
  • 112
    • 33746910190 scopus 로고    scopus 로고
    • Pegaptanib for the treatment of age-related macular degeneration
    • Zhou, B. and Wang, B. (2006) Pegaptanib for the treatment of age-related macular degeneration. Exp. Eye Res. 83, 615-619.
    • (2006) Exp. Eye Res , vol.83 , pp. 615-619
    • Zhou, B.1    Wang, B.2
  • 113
    • 48349105059 scopus 로고    scopus 로고
    • Novel dual inhibitory function aptamer-siRNA delivery system for HIV-1 therapy
    • Zhou, J., Li, H., Li, S., Zaia, J. and Rossi, J. J. (2008) Novel dual inhibitory function aptamer-siRNA delivery system for HIV-1 therapy. Mol. Ther. 16, 1481-1489.
    • (2008) Mol. Ther , vol.16 , pp. 1481-1489
    • Zhou, J.1    Li, H.2    Li, S.3    Zaia, J.4    Rossi, J.J.5
  • 114
    • 80355135153 scopus 로고    scopus 로고
    • Development of cell-type specific anti-HIV gp120 aptamers for siRNA delivery
    • Zhou, J., Li, H., Zhang, J., Piotr, S. and Rossi J. (2011a) Development of cell-type specific anti-HIV gp120 aptamers for siRNA delivery. J. Vis. Exp. 23, 2954.
    • (2011) J. Vis. Exp , vol.23 , pp. 2954
    • Zhou, J.1    Li, H.2    Zhang, J.3    Piotr, S.4    Rossi, J.5
  • 115
    • 84871919249 scopus 로고    scopus 로고
    • Functional in vivo delivery of multiplexed anti-HIV-1 siRNAs via a chemically synthesized aptamer with a sticky bridge
    • Zhou, J., Neff, C. P., Swiderski, P., Li, H., Smith, D. D., Aboellail, T., Remling-Mulder, L., Akkina, R. and Rossi, J. J. (2013) Functional in vivo delivery of multiplexed anti-HIV-1 siRNAs via a chemically synthesized aptamer with a sticky bridge. Mol. Ther. 21, 192-200.
    • (2013) Mol. Ther , vol.21 , pp. 192-200
    • Zhou, J.1    Neff, C.P.2    Swiderski, P.3    Li, H.4    Smith, D.D.5    Aboellail, T.6    Remling-Mulder, L.7    Akkina, R.8    Rossi, J.J.9
  • 116
    • 79958716788 scopus 로고    scopus 로고
    • Dual functional RNA nanoparticles containing phi29 motor pRNA and anti-gp120 aptamer for cell-type specific delivery and HIV-1 inhibition
    • Zhou, J., Shu, Y., Guo, P., Smith, D. D. and Rossi, J. J. (2011b) Dual functional RNA nanoparticles containing phi29 motor pRNA and anti-gp120 aptamer for cell-type specific delivery and HIV-1 inhibition. Methods 54, 284-294.
    • (2011) Methods , vol.54 , pp. 284-294
    • Zhou, J.1    Shu, Y.2    Guo, P.3    Smith, D.D.4    Rossi, J.J.5
  • 117
    • 84867290576 scopus 로고    scopus 로고
    • Nucleic acid aptamers: An emerging frontier in cancer therapy
    • Zhu, G., Ye, M., Donovan, M. J., Song, E., Zhao, Z. and Tan, W. (2012A) Nucleic acid aptamers: an emerging frontier in cancer therapy. Chem. Commun. (Camb) 48, 10472-10480.
    • (2012) Chem. Commun. (Camb) , vol.48 , pp. 10472-10480
    • Zhu, G.1    Ye, M.2    Donovan, M.J.3    Song, E.4    Zhao, Z.5    Tan, W.6
  • 118
    • 84867997347 scopus 로고    scopus 로고
    • Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-medi-ated specific delivery of siRNA
    • Zhu, Q., Shibata, T., Kabashima, T. and Kai, M. (2012B) Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-medi-ated specific delivery of siRNA. Eur. J. Med. Chem. 56, 396-399.
    • (2012) Eur. J. Med. Chem , vol.56 , pp. 396-399
    • Zhu, Q.1    Shibata, T.2    Kabashima, T.3    Kai, M.4


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