Development of cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Journal of Visualized Experiments

Volumn , Issue 52, 2011, Pages

  Zhou, Jiehua ^a   Li, Haitang ^a   Zhang, Jane ^a   Piotr, Swiderski ^b   Rossi, John ^a  

^a Beckman Research Institute of City of Hope   (United States)

^b Shared Resource DNA/RNA Peptide   (United States)

Author keywords

Cell type specific delivery; HIV 1 gp120; Immunology; Issue 52; RNA aptamer; RNAi (RNA interference); SELEX (systematic evolution of ligands by exponential enrichment); SiRNA (small interfering RNA)

Indexed keywords

EID: 80355135153     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2954     Document Type: Article

References (14)

1. Tuerk, C. & Gold, L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249, 505-10 (1990).
2. Ellington, A. D. & Szostak, J. W. In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818-22 (1990).
3. Robertson, D. L. & Joyce, G. F. Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA. Nature 344, 467-8 (1990).
4. Fitzwater, T. & Polisky, B. A SELEX primer. Methods Enzymol 267, 275-301 (1996).
5. Weiss, C. D. & White, J. M. Characterization of stable Chinese hamster ovary cells expressing wild-type, secreted, and glycosylphosphatidylinositol-anchored human immunodeficiency virus type 1 envelope glycoprotein. J Virol 67, 7060-6 (1993).
6. Vodicka, M. A. et al. Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses. Virology 233, 193-8 (1997).
7. Zhou, J. et al. Selection, characterization and application of new RNA HIV gp 120 aptamers for facile delivery of Dicer substrate siRNAs into HIV infected cells. Nucleic Acids Res (2009).
8. Mayer, G. The chemical biology of aptamers. Angew Chem Int Ed Engl 48, 2672-89 (2009).
9. Famulok, M., Hartig, J. S. & Mayer, G. Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy. Chem Rev 107, 3715-43 (2007).
10. Chu, T. C., Twu, K. Y., Ellington, A. D. & Levy, M. Aptamer mediated siRNA delivery. Nucleic Acids Res 34, e73 (2006).
11. McNamara, J. O., 2nd et al. Cell type-specific delivery of siRNAs with aptamer-siRNA chimeras. Nat Biotechnol 24, 1005-15 (2006).
12. Dassie, J. P. et al. Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors. Nat Biotechnol 27, 839-49 (2009).
13. Zhou, J. & Rossi, J. J. The therapeutic potential of cell-internalizing aptamers. Curr Top Med Chem 9, 1144-57 (2009).
14. Zhou, J., Li, H., Li, S., Zaia, J. & Rossi, J. J. Novel dual inhibitory function aptamer-siRNA delivery system for HIV-1 therapy. Mol Ther 16, 1481-9 (2008).