Multi-mycotoxin determination in baby foods and in vitro combined cytotoxic effects of aflatoxin M and ochratoxin A

World Mycotoxin Journal

Volumn 6, Issue 4, 2013, Pages 375-388

  Tavares, A M ^a   Alvito, P ^a,b   Loureiro, S ^c   Louro, H ^a   Silva, M J ^a  

^a NATIONAL INSTITUTE OF HEALTH DR RICARDO JORGE   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

^c UNIVERSITY OF AVEIRO   (Portugal)

Author keywords

aflatoxin Mochratoxin A; cytotoxicity; HPLC FLD; mixtures modelling

Indexed keywords

AFLATOXIN B1; AFLATOXIN B2; AFLATOXIN G1; AFLATOXIN G2; AFLATOXIN M1; BROMINE; CHLOROFORM; OCHRATOXIN;

ANTIBODY AFFINITY; ARTICLE; ARTIFICIAL MILK; BABY FOOD; CELL STRAIN CACO 2; CYTOTOXICITY; FLOUR; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; LIMIT OF QUANTITATION; LIQUID LIQUID EXTRACTION; QUALITY CONTROL; VALIDATION STUDY;

EID: 84885974209     PISSN: 18750710     EISSN: 18750796     Source Type: Journal    
DOI: 10.3920/WMJ2013.1554     Document Type: Article

References (54)

1. Alvito, P.C., Sizoo, E.A., Almeida, C.M.M. and Van Egmond, H.P., 2010. Occurrence of aflatoxins and ochratoxin A in baby foods in Portugal. Food Analytical Methods 3: 22-30.
2. Audebert, M., Riu, A., Jacques, C., Hillenweck, A., Jamin, E.L., Zalko, D. and Cravedi, J.P., 2010. Use of the gamma H2AX assay for assessing the genotoxicity of polycyclic aromatic hydrocarbons in human cell lines. Toxicology Letters 199: 182-192.
3. Baydar, T., Erkekoglu, P., Sipahi, H. and Sahin, G., 2007. Aflatoxin B and ochratoxin A levels in infant formulae and baby foods marketed in Ankara, Turkey. Journal of Food and Drug Analysis 15: 89-92.
4. Bazin, E., Mourot, A., Humpage, A.R. and Fessard, V., 2010. Genotoxicity of a freshwater cyanotoxin, cylindrospermopsin, in two human cell lines: Caco-2 and HepaRG. Environmental and Molecular Mutagenesis 51: 251-259
5. Bennett, J.W. and Klich, M., 2003. Mycotoxins. Clinical Microbiology Reviews 16: 497-516.
6. Bliss, C.I, 1939. The toxicity of poisons applied jointly. Annals of Applied Biology 26: 585-616.
7. Bony, S., Carcelen, M., Olivier, L. and Devaux, A., 2006. Genotoxicity assessment of deoxynivalenol in the Caco-2 cell line model using the Comet assay. Toxicology Letters 166: 67-76.
8. Bouaziz, C., El Dein, O.S., El Golli, E., Abid-Essefi, S., Brenner, C., Lemaire, C. and Bacha, H., 2008. Different apoptotic pathways induced by zearalenone, T-2 toxin and ochratoxin A in human hepatoma cells. Toxicology 254: 19-28.
9. Bouslimi, A., Bouaziz, C., Ayed-Boussema, I., Hassen, W. and Bacha, H., 2008. Individual and combined effects of ochratoxin A and citrinin on viability and DNA fragmentation in cultured Vero cells and on chromosome aberrations in mice bone marrow cells. Toxicology 251: 1-7.
10. Brera, C., Debegnach, F., De Santis, B., Pannunzi, E., Berdini, C., Prantera, E., Gregori, E. and Miraglia, M., 2011. Simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika by HPLC with fluorescence detection: a single-laboratory validation study. Talanta 83: 1442-1446.
11. Caloni, F., Cortinovis, C., Pizzo, F. and De Angelis, I., 2012. Transport of aflatoxin M in human intestinal Caco-2/TC7 cells. Frontiers in Pharmacology 3: 1-8.
12. Caloni, F., Stammati, A., Frigge, G. and De Angelis, I., 2006. Aflatoxin M absorption and cytotoxicity on human intestinal in vitromodel. Toxicon 47: 409-415.
13. Corcuera, L.A., Arbillaga, L., Vettorazzi, A., Azqueta, A. and Lpez de Cerain, A., 2011. Ochratoxin A reduces aflatoxin B induced DNA damage detected by the comet assay in Hep G cells. Food and Chemical Toxicology 49: 2883-2889.
14. Creppy, E.E., Chiarappa, P., Baudrimont, P., Moukha, S. and Carratu, M.R., 2004. Synergistic effects of fumonisin B and ochratoxin A: are in vitrocytotoxicity data predictive of in vivoacute toxicity? Toxicology 201: 115-123.
15. El Golli-Bennour, E.E., Kouidhi, B., Bouslimi, A., Abid-Essefi, S., Hassen, W. and Bacha, H., 2010. Cytotoxicity and genotoxicity induced by aflatoxin B, ochratoxin A, and their combination in cultured Vero cells. Journal of Biochemical and Molecular Toxicology 24: 42-50.
16. El-Tras, W.F., El-Kady, N.N. and Tayel, A.A., 2011. Infants exposure to aflatoxin M as a novel foodborne zoonosis. Food and Chemical Toxicology 49: 2816-2819.
17. European Commission (EC), 2006a. Commission Regulation (EC) No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Official Journal of the European Union L 70: 12-34.
18. European Commission (EC), 2006b. Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union L 364: 5-24.
19. Faucet-Marquis, V., Pont, F., Strmer, F., Rizk, T., Castegnaro, M. and Pfohl-Leszkowicz, A., 2006. Evidence of a new dechlorinated OTA derivative formed in opossum kidney cell cultures after pre-treatment by modulators of glutathione pathways. Correlation with DNA adducts formation. Molecular Nutrition Food Research 50: 531-542.
20. Gmez-Arranz, E. and Navarro-Blasco, I., 2010. Aflatoxin M in Spanish infant formulae: occurrence and dietary intake regarding type, protein-base and physical state. Food Additives and Contaminants Part B 3: 193-199.
21. Grenier, B. and Oswald, I.P., 2011. Mycotoxin co-contamination of food and feed: meta-analysis of publications describing toxicological interactions. World Mycotoxin Journal 4: 285-313.
22. Grosse, Y., Baudrimont, I., Castegnaro, M., Creppy, E.E., Dirheimer, G. and Pfohl-Leszkowicz, A., 1995. Ochratoxin A metabolites and DNA-adducts formation in monkey kidney cell. Chemico-Biological Interactions 95: 175-187.
23. Hadjeba-Medjdoub, K., Tozlovanu, M., Pfohl-Leszkowicz, A., Frenette, C., Paugh, R.J. and Manderville, R.A., 2012. Structure-activity relationships imply different mechanisms of action for ochratoxin A-mediated cytotoxicity and genotoxicity. Chemical Research in Toxicology 25: 181-190.
24. Heidtmann-Bemvenuti, R., Mendes, G.L., Scaglioni, P.T., Badiale-Furlong, E. and Souza-Soares, L.A., 2011. Biochemistry and metabolism of mycotoxins: a review. African Journal of Food Science 5: 861-869.
25. Hernndez-Martnez, R. and Navarro-Blasco, I., 2010. Aflatoxin levels and exposure assessment of Spanish infant cereals. Food Additives and Contaminants Part B 3: 275-288.
26. Heussner, A.H., Dietrich, D.R. and O'Brien, E., 2006. In vitroinvestigation of individual and combined cytotoxic effects of ochratoxin A and other selected mycotoxins on renal cells. Toxicology in Vitro20: 332-341.
27. Hussein, H.S. and Brasel, J.M., 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology 167: 101-134.
28. International Agency for Research on Cancer (IARC), 2002. Aflatoxins. In: Monograph on the evaluation of carcinogenic risks to humans. Vol. 82. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC, Lyon, France, pp. 171-274.
29. International Organization for Standardization (ISO), 1990. ISO 8466-1. Water quality - Calibration and evaluation of analytical methods and estimation of performance characteristics. Part 1: statistical evaluation of the linear calibration function. ISO standards compendium - Environmental water quality. Vol. 1. ISO, Geneva, Switzerland.
30. Jonker, M.J., Piskiewick, A.M., Castell, N.I. and Kammenga, J.E., 2004. Toxicity of binary mixtures of cadmium-copper and carbendazim-copper to the nematode Caenorhabditis elegans. Environmental Toxicology and Chemistry 23: 1529-1537.
31. Jonker, M.J., Svendsen, C., Bedaux, J.J.M., Bongers, M. and Kammenga, J.E., 2005. Significance testing for synergistic/antagonistic, dose level-dependent, or dose ration-dependent effects in mixture dose-response analysis. Environmental Toxicology and Chemistry 24: 2701-2713.
32. Klaric, M.S., Darabos, D., Rozgaj, R., Kasuba, V. and Pepeljnjak, S., 2010. Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action. Archives in Toxicology 84: 641-650.
33. Loewe, S. and Muischnek, H., 1926. Combinated effects I. Announcement - implements to the problem. Naunyn-Schmiedebergs Archiv fr Experimentelle Pathologie und Pharmakologie 114: 313-326.
34. Loureiro, S., Svendsen, C., Ferreira, L.G., Pinheiro, C., Ribeiro, F. and Soares, A.M.V.M., 2010. Toxicity of three binary mixtures to Daphnia magna: comparing chemical modes of action and deviations from conceptual models. Environmental Toxicology and Chemistry 29: 1716-1726.
35. Manderville, R. and Pfohl-Leszkowicz, A., 2008. Bioactivation and DNA adduction as a rationale for ochratoxin A carcinogenesis. World Mycotoxin Journal 1: 357-367.
36. Maresca, M. and Fantini, J., 2010. Some food-associated mycotoxins as potential risk factors in humans predisposed to chronic intestinal inflammatory diseases. Toxicon 56: 282-294.
37. Marin-Kuan, M., Ehrlich, V., Delatour, T., Cavin, C. and Schilter, B., 2011. Evidence for a role of oxidative stress in the carcinogenicity of ochratoxin A. Journal of Toxicology 2011: 645361.
38. Meucci, V., Razzuoli, E., Soldani, G. and Massart, F., 2010. Mycotoxin detection in infant formula milks in Italy. Food Additives and Contaminants Part A 27: 64-71.
39. Muscarella, M., Iammarino, M., Nardiello, D., Lo Magro, S., Palermo, C., Centonze, D. and Palermo, D., 2009. Validation of a confirmatory analytical method for the determination of aflatoxins B, B, G and G in foods and feed materials by HPLC with on-line photochemical derivatization and fluorescence detection. Food Additives and Contaminants Part A 26: 1402-1410.
40. Neal, G.E., Eaton, D.L., Judah, D.J. and Verma, A., 1998. Metabolism and toxicity of aflatoxins M and B in human-derived in vitrosystems. Toxicology and Applied Pharmacology 151: 152-158.
41. Pfohl-Leszkowicz, A. and Manderville, R.A., 2012. An update on direct genotoxicity as a molecular mechanism of ochratoxin A carcinogenicity. Chemical Research in Toxicology 25: 252-262.
42. Pfohl-Leszkowicz, A., Bartsch, H., Azmar, B., Mohr, U., Estève, J. and Castegnaro, M., 2002. MESNA protects rats against nephrotoxicity but not carcinogenicity induced by ochratoxin A, implicating two separate pathways. Facta Universitatis Series Medicine and Biology 9: 57-63.
43. Pfohl-Leszkowicz, A., Tozlovanu, M., Manderville, R., Peraica, M., Castegnaro, M. and Stefanovic, V., 2007. New molecular and field evidences for the implication of mycotoxins but not aristolochic acid in human nephropathy and urinary tract tumor. Molecular Nutrition Food Research 51: 131-146.
44. Repetto, G., Del Peso, A. and Zurita, J.L., 2008. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols 3: 1125-1131.
45. Rudzok, S., Schlink, U., Herbarth, O. and Bauer, M., 2010. Measuring and modelling of binary mixture effects of pharmaceuticals and nickel on cell viability/cytotoxicity in the human hepatoma derived cell line HepG. Toxicology and Applied Pharmacology 244: 336 343.
46. Ruiz, M.J., Franzova, P., Juan-Garca, A. and Font, G., 2011b. Toxicological interactions between the mycotoxins beauvericin, deoxynivalenol and T-2 toxin in CHO-K1 cells in vitro. Toxicon 58: 315-26.
47. Ruiz, M.J., Mackov, P., Juan-Garca, A. and Font, G., 2011a. Cytotoxic effects of mycotoxin combinations in mammalian kidney cells. Food and Chemical Toxicology 49: 2718-2724.
48. Shephard, G.S., Berthiller, F., Burdaspal, P.A., Crews, C., Jonker, M.A., Krska, R., MacDonald, S., Malone, R.J., Maragos, C., Sabino, M., Solfrizzo, M., Van Egmond, H.P. and Whitaker, T.B., 2012. Developments in mycotoxin analysis: an update for 2010-2011. World Mycotoxin Journal 5: 3-30.
49. Sherif, S.O., Salama, E.E. and Abdel-Wahhab, M.A., 2009. Mycotoxins and child health: the need for health risk assessment. International Journal of Hygiene and Environmental Health 212: 347-368.
50. Shibahara, T., Ogawa, H.I., Ryo, H. and Fujikawa, K., 1995. DNA-damaging potency and genotoxicity of aflatoxin M in somatic cells in vivoof Drosophila melanogaster. Mutagenesis 10: 161-164.
51. Tozlovanu, M., Canadas, D., Pfohl-Leszkowicz, A., Frenette, C., Paugh, R.J. and Manderville, R.A., 2012. Glutathione conjugates of ochratoxin A as biomarkers of exposure. Arhiv Za Higijenu Rada I Toksikologiju 63: 417-427.
52. Turner, N.W., Subrahmanyam, S. and Piletsky, S.A., 2009. Analytical methods for determination of mycotoxins: a review. Analytica Chimica Acta 632: 168-180.
53. Van Trijp, J. and Roos, A., 1991. Model for the calculation of calibration curves. RIKILT report 91.02, RIKILT-DLO, Wageningen, the Netherlands.
54. Wild, C.P. and Turner, P.C., 2002. The toxicology of aflatoxins as a basis for public health decisions. Mutagenesis 17: 471-481.