Semi-rational engineering of cytochrome P450sca-2 in a hybrid system for enhanced catalytic activity: Insights into the important role of electron transfer

Biotechnology and Bioengineering

Volumn 110, Issue 11, 2013, Pages 2815-2825

  Ba, Lina ^a   Li, Pan ^a,b   Zhang, Hui ^a   Duan, Yan ^a   Lin, Zhanglin ^a,b  

^a TSINGHUA UNIVERSITY   (China)

^b Clinical Laboratory of Zhongke   (China)

Author keywords

Cytochrome P450sca 2; Electron transfer; Iterative saturation mutagenesis; Pravastatin; Redox partner; Site directed saturation mutagenesis

Indexed keywords

BIOTRANSFORMATION PROCESS; ELECTRON TRANSFER; ENHANCED CATALYTIC ACTIVITY; KINETIC CHARACTERIZATION; PRAVASTATIN; REDOX PARTNERS; SATURATION MUTAGENESIS; WHOLE-CELL BIOTRANSFORMATIONS;

BINDING SITES; ELECTRON TRANSITIONS; HYBRID SYSTEMS; MUTAGENESIS;

INTERFACES (MATERIALS);

COMPACTIN; CYTOCHROME P450; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BIOTRANSFORMATION; CATALYSIS; ELECTRON TRANSPORT; ENZYME PURIFICATION; HYDROXYLATION; MUTAGENESIS; NONHUMAN; PROTEIN EXPRESSION;

STREPTOMYCES CARBOPHILUS;

CYTOCHROME P450SCA-2; ELECTRON TRANSFER; ITERATIVE SATURATION MUTAGENESIS; PRAVASTATIN; REDOX PARTNER; SITE-DIRECTED SATURATION MUTAGENESIS;

BINDING SITES; BIOTRANSFORMATION; CYTOCHROME P-450 ENZYME SYSTEM; KINETICS; MUTAGENESIS, SITE-DIRECTED; PRAVASTATIN; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STREPTOMYCES;

EID: 84884533513     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24960     Document Type: Article

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